REAL-TIME PCR IN THE DIAGNOSIS OF INVASIVE MENINGOCOCCAL DISEASE IN THE CZECH REPUBLIC

2017 ◽  
Author(s):  
Zuzana Vackova
Keyword(s):  
Czech Republic ◽  
Real Time ◽  
Meningococcal Disease ◽  
Real Time Pcr ◽  
Invasive Meningococcal Disease ◽  
The Czech Republic

scholarly journals Antimeningococcal herd immunity in the Czech Republic – influence of an emerging clone, Neisseria meningitidis ET-15/37

1999 ◽  
Vol 123 (2) ◽  
pp. 193-200 ◽  
Author(s):  
P. KRIZ ◽  
B. KRIZ ◽  
E. SVANDOVA ◽  
M. MUSILEK
Keyword(s):  
Czech Republic ◽  
Neisseria Meningitidis ◽  
Meningococcal Disease ◽  
Antigenic Variation ◽  
Herd Immunity ◽  
Age Distribution ◽  
Invasive Meningococcal Disease ◽  
The Czech Republic

For many years, invasive meningococcal disease in the Czech Republic occurred sporadically and was caused mainly by meningococci of serogroup B. In 1993, when a new clone (ET-15/37) emerged, the only phenotype found was C:2a:P1.2,5. In 1995, an antigenic variation of the ET-15/37 clone, B:2a:P1.2,5, occurred. The results of immunological surveys conducted in 1989 and 1996 were compared. A significantly higher proportion of 1996 sera than those collected in 1989 showed bactericidal antibodies against N. meningitidis B:2a:P1.2,5 (19·7 vs. 5·1%) and N. meningitidis C:2a:P1.2,5 (15·9 vs. 7·4%), consistent with increased herd immunity due to the spread of the new clone in the Czech Republic. There were differences in the age distribution of the positive sera.

scholarly journals Surveillance of invasive meningococcal disease in the Czech Republic

2004 ◽  
Vol 9 (11) ◽  
pp. 11-12 ◽  
Author(s):  
P Kriz
Keyword(s):  
Czech Republic ◽  
Neisseria Meningitidis ◽  
Meningococcal Disease ◽  
Western Europe ◽  
Vaccination Strategy ◽  
Invasive Disease ◽  
Invasive Meningococcal Disease ◽  
Mortality Data ◽  
Meningococcal Infections ◽  
The Czech Republic

Routine notification of invasive meningococcal disease has a long tradition in the Czech Republic: mortality data are available from 1921 and morbidity data from 1943. The collection of Neisseria meningitidis strains kept in the NRL for Meningococcal Infections in Prague dates from 1970 onwards, and represents more than 3500 strains isolated from invasive disease and their contacts, from healthy carriers and from respiratory infection. Analysis of these strains showed that the Czech meningococcal population is different from that seen in western Europe. In 1993, the incidence serogroup C meningococcal disease increased and was associated with the emergence of the hypervirulent complex Neisseria meningitidis C, ST-11, ET-15/37, and caused an increase in the incidence of invasive meningococcal disease which peaked in 1995 (2.2/100 000). A vaccination strategy targeting the part of the population at highest risk of invasive meningococcal disease was adopted in the country.

scholarly journals Exploratory Epidemiological Study on Porcine Circovirus Type 2 Infection and Postweaning Multisystemic Wasting Syndrome in the Czech Republic

2010 ◽  
Vol 79 (1) ◽  
pp. 81-90 ◽  
Author(s):  
Radek Ficek ◽  
Ivan Pšikal ◽  
Petr Fictum ◽  
Jindřiška Bendová ◽  
Eva Kosinová ◽  
...  
Keyword(s):  
Czech Republic ◽  
Lymph Nodes ◽  
Real Time ◽  
Epidemiological Study ◽  
Real Time Pcr ◽  
Single Point ◽  
Postweaning Multisystemic Wasting Syndrome ◽  
Porcine Circovirus ◽  
The Czech Republic ◽  
Wasting Syndrome

The objective of our study was to diagnose the postweaning multisystemic wasting syndrome (PMWS) and to determine the prevalence of the disease in 33 swine herds in the Czech Republic using the results of laboratory examinations of 100 pigs expressing the signs of wasting at the end of 2007. Microscopic lesions associated with the presence of porcine circovirus 2 (PCV2) antigen were detected in the lymph nodes from 39 of 100 diseased pigs (39%). Based on individual assessment of severity of microscopic lymphoid lesions associated with high amounts of PCV2 antigen, PMWS was confirmed in 4 out of 39 pigs originating from 3 of 33 herds (9%). The epidemiological study indicates that PCV2 infections associated with PMWS disease are only sporadically present in the Czech Republic. Subsequently used real time PCR technique confirmed the relation between PMWS status at the individual pig level and PCV2 DNA concentration. PCV2 DNA load in lymph nodes of PMWS-affected pigs were about 3 logs higher than the levels detected in the PMWS-nonaffected group (P < 0.05). Other parallel viral infections (PRRSV, PPV) were detected by real time PCR techniques in 21 out of 39 PCV2 infected pigs (54%). The results of serological examination of blood samples collected during the necropsy of 100 pigs are suggestive of great prevalence of PCV2 infections in pig herds; nevertheless serum samples collected from individual pigs at a single point in time had a low diagnostic value.

Cluster of three cases of invasive meningococcal disease in a preschool facility in West Bohemia, the Czech Republic

2012 ◽  
Vol 58 (2) ◽  
pp. 123-126 ◽  
Author(s):  
P. Pazdiora ◽  
I. Morávková ◽  
T. Bergerová ◽  
V. Štruncová ◽  
P. Křížová ◽  
...  
Keyword(s):  
Czech Republic ◽  
Meningococcal Disease ◽  
Invasive Meningococcal Disease ◽  
The Czech Republic ◽  
West Bohemia

scholarly journals NEW POSSIBILITIES OF CALCULATING THE VOLUMES OF EXTRACTED MASSES IN THE SURFACE MINING OF BROWN COAL

2017 ◽  
Vol 63 (3) ◽  
pp. 8-17
Author(s):  
Dana Vrublová ◽  
Roman Kapica ◽  
Beáta Gibesová ◽  
Jaroslav Mudruňka
Keyword(s):  
Czech Republic ◽  
Real Time ◽  
Coal Mining ◽  
Brown Coal ◽  
Surface Mining ◽  
Spatial Position ◽  
Mining Technology ◽  
The Czech Republic ◽  
Time Motion ◽  
Volume Calculations

Abstract The companies engaged in brown coal mining are looking for ways of managing the mining process as efficiently as possible. The principal mining technology used for brown coal mining in our country, but also in Germany and Poland, are wheel excavators. The evolving GNSS technology has enabled designing and realisation of systems for determining the spatial position of the excavator wheel. The visualisation of the wheel's spatial position and tracking of its real-time motion is performed in the Czech Republic by the program Mine Model developed by the company KVASoftware. One of the most important tasks of mine surveyors is the calculation of the volumes of the extracted masses. The described system performs this task in real-time. This article describes an application that is used to automate volume calculations.

scholarly journals Increase in meningococcal disease associated with the emergence of a novel ST-11 variant of serogroup C Neisseria meningitidis in Victoria, Australia, 1999–2000

2002 ◽  
Vol 128 (1) ◽  
pp. 7-14 ◽  
Author(s):  
D. E. TRIBE ◽  
A. M. ZAIA ◽  
J. M. GRIFFITH ◽  
P. M. ROBINSON ◽  
H. Y. LI ◽  
...  
Keyword(s):  
Czech Republic ◽  
Dna Sequencing ◽  
Neisseria Meningitidis ◽  
Meningococcal Disease ◽  
Age Distribution ◽  
Housekeeping Gene ◽  
Restriction Pattern ◽  
The Novel ◽  
Age Group ◽  
The Czech Republic

In the years 1999–2000, there was an increase in the incidence of meningococcal disease in Victoria, largely caused by Neisseria meningitidis serogroup C. This change was associated with a shift in age distribution of cases, with relatively more disease appearing in the 15–29 year age group, and with 40/58 serogroup C isolates in 2000 exhibiting a new macrorestriction pattern (pattern A). Thirty-four of 52 pattern A isolates tested displayed the novel phenotype C:2a:P1.4, and were consistently porA VR type P1.7-2,4 by DNA sequencing. Nine of 10 representative pattern A isolates analysed displayed a housekeeping gene allele profile (ST-11) that is characteristic of the electrophoretic type (ET)-15 variant that has caused outbreaks in Canada, the Czech Republic and Greece. Meningococci belonging to the ST-11 complex that were isolated in Victoria prior to 1999 did not display either restriction pattern A or PorA VR type P1.7-2,4.

Sign in / Sign up

Export Citation Format

Share Document