In vitro determination of Antibacterial Activity of Phenolic Compounds Extracted from Olive Oil on Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Fusobacterium nucleatum.

Author(s):  
Wahidatunur Musa
2021 ◽  
Vol 18 ◽  
Author(s):  
Khalida Bouarroudj-Hamici ◽  
Soraya Mettouchi ◽  
Lynda Medjkouh-Rezzak ◽  
Romain Larbat ◽  
Abderezak Tamendjari

Background: The olive tree (Olea europaea L.), the most widespread plant species in the Mediterranean basin, includes two forms: cultivated (var Europaea) and wild (var Sylvestris). Wild olive trees or oleasters cover large areas in Algeria. It has been shown that oil from oleaster has a higher content of phenolic compounds, which could have antimicrobial properties. Objective:: The objective of this study was to assess the antibacterial activity of phenolic extracts from four Algerian oleaster oils and an extra virgin olive oil (EVOO) from Chemlal variety. Methods: Phenolic compounds were determined by UHPLC-MS. Antibacterial activity was tested against six referenced human enteropathogenic bacteria by the agar disc diffusion method by measuring the diameters of the zone of inhibition. Results: The results revealed a similarity between the phenolic composition of oleasters 1 and 3 and between oleaster 4 and EVOO; however, the phenolic composition of oleaster 2 that the poorer was markedly different with a higher content of free phenolic alcohols and lower in secoiridoids.


2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Karine Lyko ◽  
Carmem Bonfimz ◽  
Elaine Machado Benelli ◽  
Cassius Carvalho Torres-Pereira ◽  
José Miguel Amenábar

Objetivo: Comparar la prevalencia de cuatro bacterias periodontopáticas incluyendo Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Fusobacterium nucleatum y Treponema denticola en muestras de placa supragingival de niños con y sin anemia de Fanconi. Material y métodos: Muestras de placa supragingival fueron colectadas en 71 personas con edades entre 6-18 años de edad. Las muestras se dividieron en tres grupos: anemia de Fanconi pre-trasplante (n= 25), anemia de Fanconi post-trasplante (n=23) y control (n=24). Las bacterias se identifi on mediante la reacción en cadena de la polimerasa para amplifi in vitro al gen codifi 16S rRNA. Resultados: El A. actinomycetemcomytans sólo fue encontrado en una muestra del grupo pretrasplante. El microorganismo P. gingivalis se identificó en una muestra del grupo pre-trasplante y en una del grupo post-trasplante. El T. denticola se encontró únicamente en dos muestras del grupo pre-trasplante. El microorganismo F. nucleatum se observó en todos los grupos. La presencia de los microorganismos varió del 30% en el grupo control al 58% en el grupo Prétrasplante. No fueron encontradas diferencias estadísticas entre los grupos. Conclusión: Los resultados del estudio sugieren que las alteraciones sistémicas encontradas en los individuos con AF no afectan la prevalencia de las cuatro bacterias analizadas.


2021 ◽  
Author(s):  
Lijuan Liu ◽  
Shengting Zhang ◽  
Xiaodan Zheng ◽  
Hongmei Li ◽  
Qi Chen ◽  
...  

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.


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