Determination the expression of miR-212 and EGFR genes in Trichophyton rubrum in affected areas in comparison with adjacent healthy spots

Lichens are demarcated as an association of two distinct and dissimilar components; the mycobiont (a fungus) and the phycobionts (an alga) forming a composite organism. The recent advancement in science has paid much attention on the biological roles of lichen secondary substances; which are attributed with a lot of biological activities, such as antibacterial, antifungal, antiviral, anti-inflammatory, anti-tumor, and antioxidant activities. The current research aims to unravel the fungicidal activity of lichen Heterodermia diademata, ethanolic extract (HDE) against prevalent superficial infection causing dermatophytes viz., Trichophyton rubrum, T. mentagrophytes, Microsporum gypseum and M. canis. The HDE was found active against M. gypseum and T. mentagrophytes with MIC; 1.68 and 1.98 mg/ml and mg/ml respectively. The dermatophytes were also analyzed by molecular phylogeny of chs-1 gene for variability in MIC and prediction of susceptible pathogens.

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Disseminated Deep Dermal Trichophyton Rubrum Infection in a Heart Transplant Recipient

The Journal of Heart and Lung Transplantation ◽

10.1016/j.healun.2021.01.1986 ◽

2021 ◽

Vol 40 (4) ◽

pp. S482

Author(s):

E.R. Woolcock ◽

F. Ara ◽

S. Bhagra ◽

C. Lewis ◽

S. Pettit ◽

...

Keyword(s):

Transplant Recipient ◽

Heart Transplant ◽

Trichophyton Rubrum ◽

Heart Transplant Recipient

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Tinea corporis caused by terbinafine‐resistant Trichophyton rubrum successfully treated with fosravuconazole

The Journal of Dermatology ◽

10.1111/1346-8138.15900 ◽

2021 ◽

Author(s):

Yurie Kitauchi ◽

Yoshiko Kumagai ◽

Yoko Inoue‐Masuda ◽

Makoto Sugiura ◽

Tomotaka Sato ◽

...

Keyword(s):

Trichophyton Rubrum ◽

Tinea Corporis

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Inhibition of Dermatophyte Fungi by Australian Jarrah Honey

Pathogens ◽

10.3390/pathogens10020194 ◽

2021 ◽

Vol 10 (2) ◽

pp. 194

Author(s):

Annabel Guttentag ◽

Krishothman Krishnakumar ◽

Nural Cokcetin ◽

Steven Hainsworth ◽

Elizabeth Harry ◽

...

Keyword(s):

Oxidative Stress ◽

Electron Microscopy ◽

Antifungal Activity ◽

Trichophyton Rubrum ◽

Microscopic Analysis ◽

Low Ph ◽

Eucalyptus Marginata ◽

Production Of Hydrogen ◽

Dermatophyte Species ◽

Scanning Electron

Superficial dermatophyte infections, commonly known as tineas, are the most prevalent fungal ailment and are increasing in incidence, leading to an interest in alternative treatments. Many floral honeys possess antimicrobial activity due to high sugar, low pH, and the production of hydrogen peroxide (H2O2) from the activity of the bee-derived enzyme glucose oxidase. Australian jarrah (Eucalyptus marginata) honey produces particularly high levels of H2O2 and has been found to be potently antifungal. This study characterized the activity of jarrah honey on fungal dermatophyte species. Jarrah honey inhibited dermatophytes with minimum inhibitory concentrations (MICs) of 1.5–3.5% (w/v), which increased to ≥25% (w/v) when catalase was added. Microscopic analysis found jarrah honey inhibited the germination of Trichophyton rubrum conidia and scanning electron microscopy of mature T. rubrum hyphae after honey treatment revealed bulging and collapsed regions. When treated hyphae were stained using REDOX fluorophores these did not detect any internal oxidative stress, suggesting jarrah honey acts largely on the hyphal surface. Although H2O2 appears critical for the antifungal activity of jarrah honey and its action on fungal cells, these effects persisted when H2O2 was eliminated and could not be replicated using synthetic honey spiked with H2O2, indicating jarrah honey contains agents that augment antifungal activity.

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