Pregnancy-related changes in bone turnover markers are not accompanied by mineral mobilisation in the appendicular skeleton in women with habitually low calcium intake.

AbstractThe aims of this study were to establish robust reference intervals and to investigate the factors influencing bone turnover markers (BTMs) in healthy premenopausal Spanish women.A total of 184 women (35–45 years) from 13 centers in Catalonia were analyzed. Blood and second void urine samples were collected between 8 a.m. and 10 a.m. after an overnight fast. Serum procollagen type I amino-terminal propeptide (PINP) and serum cross-linked C-terminal telopeptide of type I collagen (CTX-I) were measured by two automated assays (Roche and IDS), bone alkaline phosphatase (bone ALP) by ELISA, osteocalcin (OC) by IRMA and urinary NTX-I by ELISA. PTH and 25-hydroxyvitamin D (25OHD) levels were measured. All participants completed a questionnaire on lifestyle factors.Reference intervals were: PINP: 22.7–63.1 and 21.8–65.5 μg/L, bone ALP: 6.0–13.6 μg/L, OC: 8.0–23.0 μg/L, CTX-I: 137–484 and 109–544 ng/L and NTX-I: 19.6–68.9 nM/mM. Oral contraceptive pills (OCPs) influenced PINP (p=0.007), and low body mass index (BMI) was associated with higher BTMs except for bone ALP. Women under 40 had higher median values of most BTMs. CTX-I was influenced by calcium intake (p=0.010) and PTH (p=0.007). 25OHD levels did not influence BTMs. Concordance between the two automated assays for PINP and particularly CTX-I was poor.Robust reference intervals for BTMs in a Southern European country are provided. The effects of OCPs and BMI on their levels are significant, whilst serum 25OHD levels did not influence BTMs. Age, calcium intake, BMI and PTH influenced CTX-I. The two automated assays for measuring PINP and CTX-I are not interchangeable.

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Interrelationship between bone turnover markers and dietary calcium intake in pregnant women: a longitudinal study

Bone ◽

10.1016/s8756-3282(03)00203-5 ◽

2003 ◽

Vol 33 (4) ◽

pp. 606-613 ◽

Cited By ~ 32

Author(s):

Susana N Zeni ◽

Carlos R Ortela Soler ◽

Araceli Lazzari ◽

Laura López ◽

Marisa Suarez ◽

...

Keyword(s):

Longitudinal Study ◽

Bone Turnover ◽

Pregnant Women ◽

Calcium Intake ◽

Bone Turnover Markers ◽

Dietary Calcium ◽

Dietary Calcium Intake ◽

Turnover Markers

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An Evaluation of the Levels of 25-Hydroxyvitamin D3 and Bone Turnover Markers in Professional Football Players and in Physically Inactive Men

Physiological Research ◽

10.33549/physiolres.932586 ◽

2014 ◽

pp. 237-243 ◽

Cited By ~ 3

Author(s):

K. SOLARZ ◽

A. KOPEĆ ◽

J. PIETRASZEWSKA ◽

F. MAJDA ◽

M. SŁOWIŃSKA-LISOWSKA ◽

...

Keyword(s):

Physical Activity ◽

Vitamin D ◽

Bone Turnover ◽

Calcium Intake ◽

Bone Turnover Markers ◽

Serum Levels ◽

Football Players ◽

Professional Football ◽

Turnover Markers ◽

Physically Inactive

Vitamin D is synthesised in the skin during exposure to sunlight and its fundamental roles are the regulation of calcium and phosphate metabolism and bone mineralisation. The aim of our study was to evaluate serum levels of 25-hydroxyvitamin D3, PTH and bone turnover markers (P1NP, OC, β-CTx, OC/β-CTx) and the intake of calcium and vitamin D in Polish Professional Football League (Ekstraklasa) players and in young men with a low level of physical activity. Fifty healthy men aged 19 to 34 years were included in the study. We showed that 25(OH)D3 and P1NP levels and OC/β-CTx were higher in the group of professional football players than in the group of physically inactive men. The daily vitamin D and calcium intake in the group of professional football players was also higher. We showed a significant relationship between 25(OH)D3 levels and body mass, body cell mass, total body water, fat-free mass, muscle mass, vitamin D and calcium intake. Optimum 25(OH)D3 levels were observed in a mere 16.7 % of the football players and vitamin D deficiency was observed in the physically inactive men. The level of physical activity, body composition, calcium and vitamin D intake and the duration of exposure to sunlight may significantly affect serum levels of 25(OH)D3.

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Biochemical Markers of Calcium and Bone Metabolism during 18 Months of Lactation in Gambian Women Accustomed to a Low Calcium Intake and in Those Consuming a Calcium Supplement1

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jcem.83.4.4737 ◽

1998 ◽

Vol 83 (4) ◽

pp. 1059-1066

Author(s):

Ann Prentice ◽

Landing M. A. Jarjou ◽

Dorothy M. Stirling ◽

Rochelle Buffenstein ◽

Susan Fairweather-Tait

Keyword(s):

Alkaline Phosphatase ◽

Bone Turnover ◽

Bone Metabolism ◽

Calcium Intake ◽

Calcium Supplementation ◽

Bone Alkaline Phosphatase ◽

Dihydroxyvitamin D ◽

1,25 Dihydroxyvitamin D ◽

Low Calcium ◽

Turnover Markers

The effect of 18 months of lactation on indexes of calcium and bone metabolism was studied in 60 Gambian women accustomed to a very low calcium intake. Half the women consumed a calcium supplement from 10 days postpartum for 52 weeks (supplement, 714 mg Ca/day; total Ca intake, 992 ± 114 mg/day), and half consumed placebo (total Ca intake, 288 ± 128 mg/day). Fasting blood and 24-h urine samples were collected at 1.5, 13, 52, and 78 weeks of lactation and analyzed for calciotropic hormones (intact PTH, 1,25-dihydroxyvitamin D, and calcitonin), bone turnover markers (osteocalcin, bone alkaline phosphatase, and urinary deoxypyridinoline), and plasma minerals (calcium and phosphate). The first months of lactation were associated with increased bone turnover and plasma phosphate, and decreased PTH and 1,25-dihydroxyvitamin D. These effects diminished by 52 weeks, although breast milk volumes remained high. The Gambians had higher PTH, 1,25-dihydroxyvitamin D, and bone formation than British women with a greater customary calcium intake. None of the biochemical indexes was affected by calcium supplementation, with the possible exception of bone alkaline phosphatase (−29% at 52 weeks; P = 0.015). These data demonstrate that lactation-associated changes in calcium and bone metabolism are physiological and are independent of dietary calcium supply in women with very low calcium intakes.

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