scholarly journals Effect of initial fermentation medium on bioacetone, biobutanol, and bioethanol (BioABE) production from fermentable sugars of Acacia mangium using Clostridium acetobutylicum YM1

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 6912-6927
Author(s):  
Rafidah Jalil ◽  
Mohd Sahaid Kalil ◽  
Norliza Abd Rahman ◽  
Abdulalati Ibrahim Al-Tabib ◽  
Aidil Abdul Hamid ◽  
...  
Keyword(s):  
Clostridium Acetobutylicum ◽  
Low Cost ◽  
White Rot Fungi ◽  
Acacia Mangium ◽  
Product Yield ◽  
Fermentation Medium ◽  
White Rot ◽  
Fermentable Sugars ◽  
Process Variables ◽  
Initial Ph

Bioacetone, biobutanol, and bioethanol (BioABE) production is dependent on the fermentable sugars produced from lignocellulosic biomass and on the composition and initial pH of the medium. Understanding these process variables and their interconnectedness could enhance the BioABE product yield. Acacia mangium is available abundantly and it is a potential feedstock for BioABE production. In this study, BioABE was produced from fermentable sugars of A. mangium using Clostridium acetobutylicum YM1. Alkaline treated A. mangium (70 °C, 3 h, 5.50 %w/v NaOH) was further hydrolyzed via enzymatic hydrolysis using a multi-enzyme of white rot fungi to convert it into fermentable sugars. Approximately 15 g/L of fermentable sugars was produced from A. mangium (100 g/L) and was used for BioABE production in comparison with glucose. Initial findings showed that only 0.94 g/L of BioABE was produced in comparison with glucose (2.86 g/L) at a pH of 6.2. Decreasing the initial pH of the medium to 4.50 increased the BioABE (2.87 g/L), and after the medium was supplemented with tryptone-yeast-acetate (TYA), the BioABE yield increased by more than 100% to 6.84 g/L. This study discovered that BioABE produced from A. mangium was comparable to using commercial glucose, thus offering high potential as a low-cost feedstock.

scholarly journals Comparison of the Ability of Several White-rot Fungi to Biobleach Acacia Oxygen-delignified Kraft Pulp

2019 ◽  
pp. 1-10
Author(s):  
Sitompul Afrida ◽  
Toshihiro Watanabe ◽  
Yutaka Tamai
Keyword(s):  
Kraft Pulp ◽  
Extracellular Enzymes ◽  
Low Cost ◽  
White Rot Fungi ◽  
Acacia Mangium ◽  
White Rot ◽  
Irpex Lacteus ◽  
Lentinus Tigrinus

Previous screening analyses demonstrated that the in vivo biobleaching activities of the white-rot fungi Irpex lacteus KB-1.1 and Lentinus tigrinus LP-7 are higher than those of Phanerochaete chrysosporium and Trametes versicolor. The purpose of the current study was to examine the production of extracellular enzymes of these four white-rot fungi grown on three types of low-cost media containing agricultural and forestry waste, and to evaluate the ability of the produced extracellular enzymes to biobleach Acacia oxygen-delignified kraft pulp (A-OKP). The biobleaching activity of extracellular fractions of I. lacteus, L. tigrinus, T. versicolor, and P. chrysosporium cultures was the most pronounced after 3 days of incubation with Acacia mangium wood powder supplemented with rice bran and 1% glucose (WRBG) with resultant Kappa number reduction of 4.4%, 6.7%, 3.3%, and 3.3%, respectively. Therefore, biobleaching ability of I. lacteus and L. tigrinus have been shown to be higher than of T. versicolor and P. chrysosporium, both in vivo and in vitro.

scholarly journals Research on the changes of physiological characteristics of algal cells in the process of algae dissolving by immobilized white rot fungi

2020 ◽  
Vol 165 ◽  
pp. 05033
Author(s):  
Guoming Zeng ◽  
Jing Luo ◽  
Xiaowan Liu ◽  
Maolan Zhang ◽  
Hengjun Tang
Keyword(s):  
Low Cost ◽  
White Rot Fungi ◽  
Physical Method ◽  
White Rot ◽  
Ecological Security ◽  
Algal Control ◽  
The Cost ◽  
Water Eutrophication ◽  
By Products ◽  
Control Water

With the increasing of water pollution, water eutrophication is seriously affecting people’s daily life and production. Therefore, it is particularly important to explore safe and efficient algae control technology. In the current algal bloom treatment methods, the physical method is not complete in algae dissolving, and the cost of algal control is high. The chemical method is easy to produce secondary pollution and toxic by-products, and the safety is not high. However, the biological method has the advantages of low cost, high ecological security and good ecological compatibility. It is considered to be a more promising method to remove algae and biological toxins, and it is also an inevitable trend to control water eutrophication in the future.

Research on the White-Rot Fungi Degradation of Lignocellulose of Straw

2012 ◽  
Vol 550-553 ◽  
pp. 1151-1155
Author(s):  
Jiang Hong Liu ◽  
Yi Tong Chen ◽  
Rui Dan Xu ◽  
Hong Sun
Keyword(s):  
Synthetic Medium ◽  
White Rot Fungi ◽  
Fermentation Medium ◽  
Inoculum Size ◽  
White Rot ◽  
Optimum Process ◽  
Process Conditions ◽  
Additional Experiment ◽  
Orthogonal Experiments ◽  
Degradation Rates

The degradation law of straw lignin by white-rot fungi was studied. The adding amount of synthetic medium, the inoculum size and the temperature were investigated by experiment of single factor and orthogonal experiments. In the 6.5g straw fermentation medium, the optimum process conditions of degrading straw lignin by white-rot fungi were as follows: the adding amount of synthetic medium was 14mL; the inoculum size was 0.8mL; the temperature was 35°C. Through the additional experiment, degradation rate of the lignin was 49.50%; the degradation rates of cellulose and hemicellulose were 38.41% and 47.88%, respectively.

scholarly journals Evaluation of laccase production by two white-rot fungi using solid-state fermentation with different agricultural and forestry residues

BioResources ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. 5287-5300
Author(s):  
Qi An ◽  
Cong-Sheng Li ◽  
Jing Yang ◽  
Si-Yu Chen ◽  
Kai-Yue Ma ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Low Cost ◽  
White Rot Fungi ◽  
White Rot ◽  
Laccase Production ◽  
Sophora Japonica ◽  
Significant Difference ◽  
Cottonseed Hull ◽  
Forestry Residues

Pleurotus ostreatus and a newly isolated Ganoderma lingzhi strain were evaluated for their laccase secretion capacity by solid-state fermentation with different agricultural and forestry residues. There was a significant difference among fungi for biosynthetic potential. In principle, the laccase secretion capacity of P. ostreatus CY 568 was stronger than that from G. lingzhi Han 500. Different species of fungi had a preference for agricultural and forestry residues. The presence of cottonseed hull and Populus beijingensis were helpful for accelerating the rate of laccase enzyme production of P. ostreatus CY 568. Cottonseed hull and corncob were useful for improving the production of laccase from G. lingzhi Han 500. Continuous and stable laccase production was found on cottonseed hull by P. ostreatus CY 568 and G. lingzhi Han 500. Maximum laccase activity obtained from P. ostreatus CY 568 on Toona sinensis, Sophora japonica, Salix babylonica, Populus beijingensis, corncob, cottonseed hull, and straw of Oryza sativa was higher than that from G. lingzhi Han 500, and was nearly 1.16-fold, 1.59-fold, 3.32-fold, 1.39-fold, 1.08-fold, 1.08-fold, and 1.36-fold, respectively. These findings will be helpful for developing new productive strains and expanding more species for industrial application to obtain efficient and low-cost laccase.

scholarly journals Degradation of carbofuran in contaminated soil by plant-microorganism combined technology

2020 ◽  
Vol 85 (1) ◽  
pp. 111-123 ◽  
Author(s):  
Xin Wang ◽  
Zhaoxing Li ◽  
Mengqin Yao ◽  
Jia Bao ◽  
Huiwen Zhang
Keyword(s):  
Contaminated Soil ◽  
Low Cost ◽  
White Rot Fungi ◽  
Living Environment ◽  
White Rot ◽  
Adsorption Method ◽  
Pollutant Concentrations ◽  
Significant Difference ◽  
Number Of Microorganisms ◽  
The Impact

With the development of modern agriculture, the pollution caused by the use of chemical fertilizers and pesticides has become a serious problem, posing a threat to human health and the living environment. Bioremediation technology is receiving more and more attention due to the safety of contaminated soil, non-secondary pollution, and low cost. In this study, white rot fungi were immobilized by the adsorption method, and the functional plants suitable for reducing carbofuran were screened by pot experiment. Based on a previous study, a combined remediation technique was established. The results showed that after 30 days, compared to the single bioremediation of carbofuran-contaminated soil, the degradation rate increased by 19 % through the corn?white rot fungi combined remediation, and by 17 % using the sorghum?white rot fungi combined remediation. The effect of the pesticide content in soil on the combined remediation is mainly reflected in the significant difference in the number of microorganisms (p < 0.05). Combined bioremediation may be a better alternative to mitigate the impact of high pollution on microorganisms at different pollutant concentrations compared to single microbial bioremediation or phytoremediation.

scholarly journals Fermentable Sugars from Agrowastes Using Cellulase Enzymes from Local White Rot Fungi Pycnoporus sanguineus

2017 ◽  
Vol 29 (2) ◽  
pp. 105-111 ◽  
Author(s):  
Mohd Sahaid Kalil ◽  
◽  
Rafidah Jalil ◽  
Norhazmiza Ahmad Safri
Keyword(s):  
White Rot Fungi ◽  
White Rot ◽  
Fermentable Sugars ◽  
Cellulase Enzymes ◽  
Pycnoporus Sanguineus

Comparative Studies of Remazol Brillant Blue Removal by Immobilized Organisms; Investigation of Metabolites by GC/MS and FTIR Spectrometry

2015 ◽  
Vol 98 (2) ◽  
pp. 445-449 ◽  
Author(s):  
Hatice Ardag Akdogan ◽  
Merve Canpolat Topuz
Keyword(s):  
Biological Treatment ◽  
Low Cost ◽  
White Rot Fungi ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
Synthetic Dyes ◽  
Viable Option ◽  
Chemical Pollutants ◽  
Ftir Spectrometry ◽  
Textile Industries

Abstract Reactive dyes are important chemical pollutants from textile industries. Treatment of effluents from dye-based industries poses a major problem, and biotreatment with white rot fungi seems to be a viable option. The biological treatment of synthetic dyes at a low cost and in the shortest possible time is used especially in dye and textile industries and leads to pollution in the wastewater dumped into the environment by these industries. For this study, decolorization of the recalcitrant dye Remazol Brilliant Blue R by immobilized Pleurotus ostreatus and Coprinus plicatilis was investigated. This dye was removed 100% (dye concentration: 10.0 mg/L) by both immobilized organisms. Extracellular ligninolytic enzyme activities were also measured during the decolorization. There was an attempt to identify metabolites with FTIR spectrometry and GC/MS at the end of the decolorization. These results indicated that the samples did not include any detectable metabolite.

scholarly journals Screening of ligninolytic fungi for biological pretreatment of lignocellulosic biomass

2015 ◽  
Vol 61 (10) ◽  
pp. 745-752 ◽  
Author(s):  
Chunyan Xu ◽  
Deepak Singh ◽  
Kathleen M. Dorgan ◽  
Xiaoyu Zhang ◽  
Shulin Chen
Keyword(s):  
Lignocellulosic Biomass ◽  
Peroxidase Activity ◽  
Low Cost ◽  
White Rot Fungi ◽  
High Potential ◽  
White Rot ◽  
Barley Straw ◽  
Laccase Production ◽  
Biological Pretreatment ◽  
Ligninolytic Fungi

To identify white rot fungi with high potential in biological pretreatment of lignocellulosic biomass, preliminary screening was carried out on plates by testing different strains for their ability to oxidize guaiacol and decolorize the dyes azure B and Poly R-478. Of the 86 strains screened, 16 were selected for secondary screening for their ligninolytic ability; however, low manganese peroxidase activity and no lignin peroxidase activity were detected. Strain BBEL0970 proved to be the most efficient in laccase production and was subsequently identified as Trametes versicolor by analysis of the ribosomal DNA internal transcribed spacer gene sequence. In combining laccase production with biological pretreatment, the replacement of glucose with barley straw significantly improved the laccase activity by up to 10.3 U/mL, which provided evidence toward potential utilization of barley straw in laccase production by BBEL0970. Simultaneously, comparison by thermogravimetric analysis of the untreated and pretreated barley straw in liquid fermentation of laccase also demonstrated the high potential of BBEL0970 in biological pretreatment of lignocellulosic biomass. This work sheds light on further exploration on the integrated process of low-cost laccase production and efficient biological pretreatment of barley straw by T. versicolor BBEL0970.

Application of white-rot fungi treated Fructus forsythiae shell residue as a low-cost biosorbent to enrich forsythiaside and phillygenin

2012 ◽  
Vol 74 ◽  
pp. 244-255 ◽  
Author(s):  
Jiao Jiao ◽  
Qing-Yan Gai ◽  
Yu-Jie Fu ◽  
Yuan-Gang Zu ◽  
Meng Luo ◽  
...  
Keyword(s):  
Low Cost ◽  
White Rot Fungi ◽  
White Rot

scholarly journals Low cost pretreatment of lignocellulosic waste by white rot fungi for ethanol production using Saccharomyces cerevisiae

2018 ◽  
Vol 9 (1) ◽  
pp. 18
Author(s):  
Thamilmaraiselvi B ◽  
Steffi PF ◽  
Sathammaipriya N ◽  
Sangeetha K
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Low Cost ◽  
White Rot Fungi ◽  
Renewable Resource ◽  
White Rot ◽  
Lignocellulosic Ethanol ◽  
Yeast Culture ◽  
Lignocellulosic Substrates ◽  
Ancient Times

Saccharomyces cerevisiae is a species of yeast. It has been instrumental in winemaking, baking, and brewing since ancient times. The present study was performed to produce lignin degrading enzymes to degrade lignocellulosic substrates and to produce ethanol using Saccharomyces cerevisiae by performing FTIR method. The yeast culture Saccharomyces cerevisiae was isolated and screened for the production of lignolytic enzymes. Then it was pretreated to produce lignocellulosic substrates. Lignocellulosic materials are considered the most abundant renewable resource available for the production of ethanol by FTIR method. The present study concluded the utilization of lignocellulosic biomass for ethanol production in future. Keywords: Saccharomyces cerevisiae; Lignocellulosic; Ethanol; FTIR

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