Evolution and enrichment of CYP5035 in Polyporales: functionality of an understudied P450 family

Bioprospecting for innovative basidiomycete cytochrome P450 enzymes (P450s) is highly desirable due to the fungi’s enormous enzymatic repertoire and outstanding ability to degrade lignin and detoxify various xenobiotics. While fungal metagenomics is progressing rapidly, the biocatalytic potential of the majority of these annotated P450 sequences usually remains concealed, although functional profiling identified several P450 families with versatile substrate scopes towards various natural products. Functional knowledge about the CYP5035 family, for example, is largely insufficient. In this study, the families of the putative P450 sequences of the four white-rot fungi Polyporus arcularius, Polyporus brumalis, Polyporus squamosus and Lentinus tigrinus were assigned, and the CYPomes revealed an unusual enrichment of CYP5035, CYP5136 and CYP5150. By computational analysis of the phylogeny of the former two P450 families, the evolution of their enrichment could be traced back to the Ganoderma macrofungus, indicating their evolutionary benefit. In order to address the knowledge gap on CYP5035 functionality, a representative subgroup of this P450 family of P. arcularius was expressed and screened against a test set of substrates. Thereby, the multifunctional enzyme CYP5035S7 converting several plant natural product classes was discovered. Aligning CYP5035S7 to 102,000 putative P450 sequences of 36 fungal species from Joint Genome Institute-provided genomes located hundreds of further CYP5035 family members, which subfamilies were classified if possible. Exemplified by these specific enzyme analyses, this study gives valuable hints for future bioprospecting of such xenobiotic-detoxifying P450s and for the identification of their biocatalytic potential. Graphical abstract Key points • The P450 families CYP5035 and CYP5136 are unusually enriched in P. arcularius. • Functional screening shows CYP5035 assisting in the fungal detoxification mechanism. • Some Polyporales encompass an unusually large repertoire of detoxification P450s.

Macroscopic Fungi Exploration in Batang Gadis National Park Resort 7 Mandailing Natal District, North Sumatra

Introduction: Macroscopic fungi are simple plants that are often found growing wild in the wild. This study aims to determine the types of macroscopic fungi, to recognize the beneficial and harmful macroscopic fungi in Batang Gadis National Park Resort Area 7. Materials and Methods: A descriptive survey method with deliberate sampling using line transects, namely using a plot with a size of 20 m x 20 m as many as 36 plots and the distance of each plot is 10 m. Results: Found in 32 types of macroscopic fungi were obtained which belong to 11 families, such as: Polyporaceae, Ganodermatacea, Marasmiaceae, Stereaceae, Hygrophoraceae, Amanitaceae, Tricholomataceae, Russulaceae, Auriculariaceae, Sparassidaceae, and Psathyellaceae. The diversity index (H') of macroscopic fungi was 2.92 (medium). There are three types of macroscopic fungi that are consumed by the human in Sopotinjak Village as food and traditional medicine, and seven types that are not consumed because they contain toxins. There are 22 other species, namely Trametes cimulata, Lentinus tigrinus (Bull) Fr, Polyporus sp., Hygrophorus psittacinus, Trymyces sp., Hygrophorus aurantica (Wulfen), Polyporus sp.2, Collybia sp., Russula sp., Trametes sp.1, Ganoderma sp, Trametes sp.2, Stereum sp., Lignosus rhinocerus, Trametes versicolor (L), Trametes sp.3, Sparasis crispa (Wulfen), Hygrocybe conica (Schaeff), Polyporus sp.3, Trametes sp.4, Stereum hirsutum (Willd) Gray, Microporus xanthopus (Fr) Kuntze, and Tametes sp.5.

Exploration of potential wild medicinal mushrooms from Pocut Meurah Intan forest park, Aceh, Indonesia

Mushroom is one of living things with abundant diversity and has various beneficial potentials; one of them is as medicine. The use of mushrooms as medicine has long been known primarily as traditionally made herbs and the knowledge has been passed down for generations. The Pocut Meurah Intan Forest Park as a conservation forest, stores a variety of mushrooms in it. Data about the type of mushroom that has medicinal potential existed in the forest park has never been published so it is important to do so. This study aims to record the types of mushrooms that have benefits as medicines. The method used is an exploratory survey through a trail or tracking path then identified as a medicinal mushroom base on literature studies. The results of the study found 15 species of mushrooms that have potential as medicine, namely Cookeina tricholoma, Sarcoscypha coccinea, Tremella fuciformis, Auricularia auricula-judae, Flammulina velutipes, Schizophyllum commune, Scleroderma citrinum, Boletus edulis, Trametes versicolor, Lentinus tigrinus, Lentinus squarosulus, Ganoderma applanatum, Ganoderma resinaceum, Thelephora ganbajun and Lycoperdon echinatum. This shows that Pocut Meurah Intan Forest Park has great resources and potential that can be utilized for the benefit of the surrounding community, and it needs to be preserved so that diversity is maintained and can be used by future generations.

KEANEKARAGAMAN JENIS JAMUR MAKROSKOPIS DI BUKIT WANGKANG DESA SUNGAI AMBAWANG KABUPATEN KUBU RAYA

Macroscopic fungi important role for ecosystem sustainable forest. Research to diversity of species macroscopic fungi in area Bukit Wangkang, Sungai Ambawang village. The research was counducted by survey method with multiple plot techniques. Laying of plots by means of purposive sampling, plots measuring 5m x 5m. obtained 23 plot observations total area of 0,0575 Ha, total research area of 3700 Ha. The research area, found 29 macroscopic fungi from 13 families, the families polyporaceae many of 10 species, then hymenochaetaceae many of 4 (four) types and ganodermataceae many of 3 (three) types. The high INP value Mycena sp (26,571), Xylaria sp 1 (23,3312) and Xylaria sp 2 (13,8331). Discovered 8 (eight) species macroscopic fungi can be consumed such as ganoderma lucidum as medicinal ingredient which processed of powder and Lentinus tigrinus as food ingredients boiled or sautéed. Macroscopic fungi cannot be consumed like rigidoporus microporus because it has fruiting body that is hard, and poisonous fungi species such as coltricia sp and microporus sp types used as souvenir. 29 species of fungi founded 4 (four) species fungi grow out of the ground and 25 species types other in dead wood. Obtained 23 plots with three types of canopy density where density rarely obtained 6 (six) species of macroscopis fungi of 4 (four) plot, then density obtained 10 of fungi of 9 (nine) plot observation. Density obrained 13 fungi of 10 plot observations.Keywords: Edible mushroom, Macroscopic fungi, Secondary forest

Comparison of the Ability of Several White-rot Fungi to Biobleach Acacia Oxygen-delignified Kraft Pulp

Previous screening analyses demonstrated that the in vivo biobleaching activities of the white-rot fungi Irpex lacteus KB-1.1 and Lentinus tigrinus LP-7 are higher than those of Phanerochaete chrysosporium and Trametes versicolor. The purpose of the current study was to examine the production of extracellular enzymes of these four white-rot fungi grown on three types of low-cost media containing agricultural and forestry waste, and to evaluate the ability of the produced extracellular enzymes to biobleach Acacia oxygen-delignified kraft pulp (A-OKP). The biobleaching activity of extracellular fractions of I. lacteus, L. tigrinus, T. versicolor, and P. chrysosporium cultures was the most pronounced after 3 days of incubation with Acacia mangium wood powder supplemented with rice bran and 1% glucose (WRBG) with resultant Kappa number reduction of 4.4%, 6.7%, 3.3%, and 3.3%, respectively. Therefore, biobleaching ability of I. lacteus and L. tigrinus have been shown to be higher than of T. versicolor and P. chrysosporium, both in vivo and in vitro.