The genus Microniphargus (Crustacea, Amphipoda): evidence for three lineages distributed across northwestern Europe and transfer from Niphargidae to Pseudoniphargidae

Microniphargus leruthi Schellenberg, 1934 (Amphipoda: Niphargidae) was first described based on samples collected in Belgium and placed in a monotypic genus within the family Niphargidae. However, some details of its morphology as well as recent phylogenetic studies suggest that Microniphargus may be more closely related to Pseudoniphargus (Amphipoda: Pseudoniphargidae) than to Niphargus. Moreover, M. leruthi ranges over 1,469 km from Ireland to Germany, which is striking since only a few niphargids have confirmed ranges in excess of 200 km. To find out the phylogenetic position of M. leruthi and check whether it may be a complex of cryptic species, we collected material from Ireland, England and Belgium then sequenced fragments of the mitochondrial cytochrome c oxidase subunit 1 gene as well as of the nuclear 28S ribosomal gene. Phylogenetic analyses of both markers confirm that Microniphargus is closer to Pseudoniphargus than to Niphargus, leading us to reallocate Microniphargus to Pseudoniphargidae. We also identify three congruent mito-nuclear lineages present respectively in Ireland, in both Belgium and England, and in England only (with the latter found in sympatry at one location), suggesting that M. leruthi is a complex of at least three species with a putative centre of origin in England.

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The genus Microniphargus (Crustacea, Amphipoda): evidence for three lineages distributed across northwestern Europe and transfer to Pseudoniphargidae

10.1101/2020.08.25.266817 ◽

2020 ◽

Author(s):

Dieter Weber ◽

Fabio Stoch ◽

Lee R.F.D. Knight ◽

Claire Chauveau ◽

Jean-François Flot

Keyword(s):

Cryptic Species ◽

Phylogenetic Analyses ◽

Ribosomal Gene ◽

Phylogenetic Position ◽

Mitochondrial Cytochrome ◽

Monotypic Genus ◽

Oxidase Subunit ◽

Centre Of Origin ◽

Phylogenetic Studies ◽

The Family

AbstractMicroniphargus leruthi Schellenberg, 1934 (Amphipoda: Niphargidae) was first described based on samples collected in Belgium and placed in a monotypic genus within the family Niphargidae. However, some details of its morphology as well as recent phylogenetic studies suggest that Microniphargus may be more closely related to Pseudoniphargus (Amphipoda: Pseudoniphargidae) than to Niphargus. Moreover, M. leruthi ranges over 1,469km from Ireland to Germany, which is striking since only a few niphargids have confirmed ranges in excess of 200km. To find out the phylogenetic position of M. leruthi and check whether it may be a complex of cryptic species, we collected material from Ireland, England and Belgium then sequenced fragments of the mitochondrial cytochrome c oxidase subunit 1 gene as well as of the nuclear 28S ribosomal gene. Phylogenetic analyses of both markers confirm that Microniphargus is closer to Pseudoniphargus than to Niphargus, leading us to reallocate Microniphargus to Pseudoniphargidae. We also identify three congruent mito-nuclear lineages present respectively in Ireland, in both Belgium and England, and in England only (with the latter found in sympatry at one location), suggesting that M. leruthi is a complex of at least three species with a putative centre of origin in England.

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Three new species of the genus Aphidius (Hymenoptera, Braconidae, Aphidiinae) from South Korea

Journal of Hymenoptera Research ◽

10.3897/jhr.86.70767 ◽

2021 ◽

Vol 86 ◽

pp. 63-77

Author(s):

Sangjin Kim ◽

Željko Tomanović ◽

Yeonghyeok Yu ◽

JuHyeong Sohn ◽

Yunjong Han ◽

...

Keyword(s):

New Species ◽

Cytochrome C ◽

South Korea ◽

Cytochrome C Oxidase ◽

Dna Barcode ◽

Biocontrol Agents ◽

Phylogenetic Position ◽

Mitochondrial Cytochrome ◽

Reference Species ◽

Three New Species

Several species of the genus Aphidius are well known as commercial biocontrol agents of pest aphids, and more than 130 species of the genus have been recorded worldwide. To date, only 15 Aphidius species have been recorded in South Korea. Using the DNA barcode region (ca. 658 bp) of mitochondrial cytochrome c oxidase subunit I (COI), we amplified sequences of 15 Aphidius Korean species, aligned them in combination with 23 reference species retrieved from GenBank for comparison and identification, and then reconstructed a barcode phylogeny by the neighbour-joining method. As a result, three Aphidius species were found to be new to Science. Descriptions and illustrations of the three species new to Science – Aphidius longicarpussp. nov., A. longistigmussp. nov., and A. asiaticussp. nov. – are provided, together with their phylogenetic position within the genus Aphidius. In addition, a redescription of A. areolatus, a parasitoid of maple aphids (Peryphillus spp.), is also given.

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Phylogenetic position of Linguatula arctica and Linguatula serrata (Pentastomida) as inferred from the nuclear 18S rRNA gene and the mitochondrial cytochrome c oxidase subunit I gene

Parasitology Research ◽

10.1007/s00436-013-3534-9 ◽

2013 ◽

Vol 112 (10) ◽

pp. 3517-3525 ◽

Cited By ~ 23

Author(s):

Bjørn Gjerde

Keyword(s):

Cytochrome C ◽

Cytochrome C Oxidase ◽

18S Rrna ◽

18S Rrna Gene ◽

Phylogenetic Position ◽

Rrna Gene ◽

Mitochondrial Cytochrome ◽

Linguatula Serrata ◽

Oxidase Subunit ◽

I Gene

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Monophyletic status of European morphogenera of the subfamily Candoninae Kaufmann, 1900 (Ostracoda: Candonidae) in relation to their mtDNA phylogenies

Journal of Crustacean Biology ◽

10.1093/jcbiol/ruz041 ◽

2019 ◽

Vol 39 (5) ◽

pp. 567-573

Author(s):

Anna Wysocka ◽

Adrianna Kilikowska ◽

Nataša Mori ◽

Sanda Iepure ◽

Michalina Kijowska ◽

...

Keyword(s):

Cytochrome C ◽

Cytochrome C Oxidase ◽

Phylogenetic Analyses ◽

Phylogenetic Position ◽

Salient Points ◽

Species Groups ◽

Mitochondrial Fragment ◽

Subterranean Waters ◽

Generic Relationships ◽

Taxonomic System

Abstract The subfamily Candoninae Kaufmann, 1900a is one of the most taxonomically difficult and species-rich lineages of non-marine Ostracoda. It is widely distributed, inhabiting all kinds of surface and subterranean waters, but its taxonomy remains unclear and generic relationships ambiguous due to a number of homoplasies. To verify the morphology-based taxonomy and monophyly of the European morphogenera of Candoninae we propose the analysis of the mitochondrial fragment encoding cytochrome c oxidase subunit I (COI). We investigated 75 individuals of 25 species representing eight of 13 extant genera of Candoninae known from Europe. The phylogenetic analyses revealed a complex pattern of relationships and a noteworthy incongruence with the currently used taxonomic system. The monophyletic status of the morphogenera CandonaBaird, 1845, FabaeformiscandonaKrstić, 1972, and PseudocandonaKaufmann, 1900a appeared questionable, but instead monophyly of some species groups distinguished morphologically within these genera seemed to be confirmed in our study. The phylogenetic position of some other morphogenera remained unresolved. Our analysis, focusing on well-supported nodes, nevertheless revealed a handful of salient points on the evolution of Candoninae that deserve further investigation.

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Morphology, Ultrastructure, and Mitochondrial Genome of the Marine Non-Photosynthetic Bicosoecid Cafileria marina Gen. et sp. nov.

Microorganisms ◽

10.3390/microorganisms7080240 ◽

2019 ◽

Vol 7 (8) ◽

pp. 240

Author(s):

Dagmar Jirsová ◽

Zoltán Füssy ◽

Jitka Richtová ◽

Ansgar Gruber ◽

Miroslav Oborník

Keyword(s):

Mitochondrial Genome ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Small Subunit ◽

Ribosomal Gene ◽

Morphological Data ◽

Phylogenetic Position ◽

Rock Surface ◽

Transmission Electron ◽

The Family

In this paper, we describe a novel bacteriophagous biflagellate, Cafileria marina with two smooth flagellae, isolated from material collected from a rock surface in the Kvernesfjorden (Norway). This flagellate was characterized by scanning and transmission electron microscopy, fluorescence, and light microscopy. The sequence of the small subunit ribosomal RNA gene (18S) was used as a molecular marker for determining the phylogenetic position of this organism. Apart from the nuclear ribosomal gene, the whole mitochondrial genome was sequenced, assembled, and annotated. Morphological observations show that the newly described flagellate shares key ultrastructural characters with representatives of the family Bicosoecida (Heterokonta). Intriguingly, mitochondria of C. marina frequently associate with its nucleus through an electron-dense disc at the boundary of the two compartments. The function of this association remains unclear. Phylogenetic analyses corroborate the morphological data and place C. marina with other sequence data of representatives from the family Bicosoecida. We describe C. marina as a new species from a new genus in this family.

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A colour variation of Clibanarius virescens (Krauss, 1843) (Decapoda, Anomura) collected from Amami Oshima Island and Okinawa, Japan

Crustaceana ◽

10.1163/15685403-00003748 ◽

2018 ◽

Vol 91 (1) ◽

pp. 85-101 ◽

Cited By ~ 1

Author(s):

Akihiro Yoshikawa ◽

Tomoyuki Nakano ◽

Takashi P. Satoh ◽

Akira Asakura

Keyword(s):

Cytochrome C ◽

Cytochrome C Oxidase ◽

Hermit Crab ◽

Phylogenetic Analyses ◽

Histone H3 ◽

Mitochondrial Cytochrome ◽

Cytochrome C Oxidase I ◽

Okinawa Island ◽

Colour Variation ◽

Nuclear Histone

During our survey on the intertidal hermit crab fauna on Amami Oshima Island and Okinawa Island, southwestern Japan, we collected specimens identified asClibanariusspecies by their morphological features, but that had an unknown coloration for the genus: they have whitish ambulatory legs and a whitish shield. For species identification of these specimens, we observed their coloration and morphology and also performed phylogenetic analyses using the genes mitochondrial cytochrome c oxidase I (COI) and nuclear histone H3. The analysis has shown that these specimens are to be identified asClibanarius virescens(Krauss, 1843).

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