Abstract
BackgroundThe antiseptic solutions most used by Eye bank or in ophthalmologic surgeries are polyvinylpyrrolidone-iodine and gluconate of chlorhexidine. Objetives: The objective of this study was to evaluate the antiseptic effect in the reduction of ocular globe microbiota from corneal donors, as well as to analyze the susceptibility of the microbiota to gentamicin. MethodsThirty pairs of corneas received antiseptics, with Povidone-iodine in the right eye and gluconate of chlorhexidine in the left eye, and for each time of action (5, 10 and 15 minutes), totalling three groups, and using 10 pairs of eyeballs to each group. Swabs were collected from the ocular surface prior to application of the solutions, after and at the time of preservation of the corneal tissue. After identification of the microbiota, an antibiogram test with gentamicin was performed. ResultsRegarding the data obtained in the second group, after the use of antiseptics, there was a reduction of 40% in the total of gram positive bacteria and a reduction of 75.9% to gram negative bacteria, the results shows that in the second group, both antiseptics were more effective to Gram negative bacteria. The third group results show the residual effect of antiseptics and there was a reduction of 99.1% of all micro-organisms. To the antibiogram test, 88% of the microorganisms isolated, were sensitive to gentamicin. ConclusionWas observed that using the tested concentration to each antissepetic, there are no action differences to descontamination between the two products, there was not differences between the action time too. Key-words: eye bank, enucleation, povidone-iodine, gluconate of chlorhexidine, transplantation.
Assessment of the human corneal endothelium: in vivo Topcon SP2000P specular microscope versus ex vivo sambacornea eye bank analyser