A Study pattern of Sero-prevalence of HIV,  HCV, HBsAg, VRDL and Maleria in the donor  blood bag screened at Blood of Bank: A three- year study

A 13-year-old patient developed severe shock due to administration of a Yersinia enterocolitica-contaminated red blood cell concentrate. Y. enterocolitica (serotype O:9, biotype II) was cultivated from the residual blood in the blood bag and from a stool sample of the blood donor. In the donor's plasma immunoglobulin M (IgM), IgA, and IgG antibodies against Yersinia outer proteins (YopM, -H, -D, and -E) were found. Since the donor remembered a short-lasting, mild diarrhea 14 days prior to blood donation, a transient attack of Yersinia enteritis may be associated with a longer than expected period of asymptomatic bacteremia that causes contamination of donor blood. Serological screening for IgM antibodies against Yersinia outer proteins might offer a way to reduce the risk of transfusion-associated Y. enterocolitica sepsis.

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Effect of Storage on Osmotic Fragility in CPDA-1 Stored Blood in Sokoto, Northwestern Nigeria

Advances in Hematology and Oncology Research ◽

10.33140/ahor.01.01.02 ◽

2018 ◽

Vol 1 (1) ◽

Keyword(s):

Osmotic Fragility ◽

Cold Chain ◽

Donor Blood ◽

Erythrocyte Osmotic Fragility ◽

Healthy Adult Male ◽

Red Cell Transfusion ◽

Blood Bag ◽

Oxygen Carrying Capacity ◽

Phosphate Buffered Saline

The aim of this study was to investigate the effects of storage on the in vitro osmotic fragility of erythrocytes of humans in a single unit of whole blood. Blood was collected by venepunture from a healthy adult male (70-75 kg) into CPDA-1(450ml) blood bag containing citrate phosphate dextrose as anticoagulant (63ml) and stored in a blood bank maintained at 4°c ± 2°c. The osmotic fragility of the erythrocytes was determined by measuring the release of haemoglobin from blood added to tubes containing serially diluted phosphate buffered saline (pH 7.4). The Blood samples were analyzed on day 1 to day 35 after collection (5 weeks). Increased erythrocyte osmotic fragility was observed at week 3 (p=0.010). The initial haemolysis (>5%) occurred between 0.50% and 0.55% PBS. The mean corpuscular fragility was between 0.35 and 0.45% PBS. Maximum haemolysis occurred in 0.35% PBS. Osmotic fragility was significantly affected by storage (p<0.05). In conclusion, this research showed that there is an increase in the osmotic fragility as donor blood is stored and that the effect is more pronounced from week 3. There is need to maintain the cold chain management of stored donor blood to ensure that the aim of red cell transfusion which is to manage anaemia and increase the oxygen carrying capacity is not compromised.

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Impact of Autologous Umbilical Cord Blood and Donor Blood Transfusion on Cytokine Profile in Neonates with Transposition of the Great Arteries

International Journal of Physiology and Pathophysiology ◽

10.1615/intjphyspathophys.v4.i2.10 ◽

2013 ◽

Vol 4 (2) ◽

pp. 83-90

Author(s):

Yanina V. Tkachenko ◽

Ganna M. Vorobiova ◽

Volodumyr A. Zhovnir ◽

Illya N. Yemets

Keyword(s):

Blood Transfusion ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Cytokine Profile ◽

Donor Blood

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Heavy metals from donor blood and breast milk products in the NICU

Pediatric Research ◽

10.1038/s41390-021-01501-x ◽

2021 ◽

Author(s):

Tarik Zahouani ◽

Katherine Pultorak ◽

Fulden Pay ◽

Alexandre E. Medina ◽

Dina E. El-Metwally

Keyword(s):

Heavy Metals ◽

Breast Milk ◽

Donor Blood ◽

Milk Products

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Development of the renal response to blood volume expansion in normal and fast-growing rats

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1976.231.3.832 ◽

1976 ◽

Vol 231 (3) ◽

pp. 832-836

Author(s):

HH Bengele ◽

S Solomon

Keyword(s):

Volume Expansion ◽

Control Period ◽

Male Rats ◽

Regulatory Function ◽

Chronological Age ◽

Donor Blood ◽

Fast Growing ◽

Growing Rats ◽

Blood Volume Expansion ◽

Diuretic Response

Male rats from reduced (fast-growing) litters between 14 and 50 days of age were studied. Standard renal clearnce techniques were employed. After a 60-min control period, the animals were infused (2.3% body wt) with heparinized donor blood obtained from lillermates of the same age. Renal function was followed for an additional 60 min. The efficiency of the diuretic response, the percent infused volume excreted above control levels, and the sodium efficiency, the percent infused Na excreted, were calculated. Results indicate that both efficiencies develop in a discontinuous pattern and that they are comparable. This pattern of development, as well as the magnitude of the mature response, is comparable to that previously reported for rats from intact (normally growing) litters. The onset and attainment of the mature response is, however, shifted in time, such that reduced-litter animals achieve the mature response 10-15 days earlier than intact-litter rats. Results exclude chronological age or body weight alone as principal determinant of the mature response and suggest that some function of growth rate is responsible for the maturation of this regulatory function.

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