scholarly journals Molecular Identification of Cucurbit Fly Dacus Ciliatus (diptera: Tephritidae) Infest Cucurbitaceae Family Based on Mitochondrial Gene in Kurdistan Region- Iraq

2019 ◽  
Vol 22 (2) ◽  
pp. 229-238
Author(s):  
GEHAN MARONSY ◽  
◽  
SHAMAL AL-MUFFTI ◽  
Keyword(s):  
Molecular Identification ◽  
Mitochondrial Gene ◽  
Kurdistan Region ◽  
Family Based ◽  
Cucurbitaceae Family ◽  
Dacus Ciliatus

scholarly journals Molecular identification and population genetic study of Elaeidobius kamerunicus Faust. (Coleoptera: Curculionidae) from Indonesia, Malaysia and Cameroon based on mitochondrial gene

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Van Basten Tambunan ◽  
Ardha Apriyanto ◽  
Walter Ajambang ◽  
Culbertson Enow Etta ◽  
Bandung Sahari ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Molecular Identification ◽  
Oil Palm ◽  
Genetic Study ◽  
Population Genetic ◽  
Mitochondrial Gene ◽  
Dna Barcode ◽  
Coi Gene ◽  
Population Genetic Study ◽  
Elaeidobius Kamerunicus

Abstract. Tambunan VB, Apriyanto A, Ajambang W, Etta CE, Sahari B, Buchori D, Hidayat P. 2020. Molecular identification and population genetic study of Elaeidobius kamerunicus Faust. (Coleoptera: Curculionidae) from Indonesia, Malaysia and Cameroon based on mitochondrial gene. Biodiversitas 21: 3263-3270. Oil palm pollinating weevil Elaeidobius kamerunicus is a very important insect pollinator in oil palm plantation. However, there is still lack of information about molecular identification and population genetic study in this species. The purpose of this study was to explore the effectiveness of oil palm pollinating weevil identification using mitochondrial DNA of COI gene and to assess its genetic variation between different locations and countries. We sequenced the DNA barcode of 36 individuals of this species using the mtDNA Cytochrome Oxidase I (COI) gene to explore their genetic variation, identity and phylogenetic relationship. The COI gene sequences generated from this study were successful in identifying E. kamerunicus. Phylogenetic analysis also revealed 3 well-supported monophyletic haplogroups of E. kamerunicus population. In addition, genetic differentiation analysis revealed that most populations from Indonesia were different from Malaysian and Cameroonian populations indicating that there was a genetic variation between the population samples from these countries. The overall E. kamerunicus used in this study were geographically structured in two regions; outside Indonesia region (Cameroon and Malaysia) and Indonesia region. These results demonstrate the feasibility of using COI gene sequence for molecular identification and population genetic study of E. kamerunicus species.

scholarly journals Multivariate analisys of species from Cucurbitaceae family

Genetika ◽  
2012 ◽  
Vol 44 (2) ◽  
pp. 227-234
Author(s):  
Emina Mladenovic ◽  
Janos Berenji ◽  
Marija Kraljevic-Balalic ◽  
Jelena Cukanovic ◽  
Ivana Blagojevic
Keyword(s):  
Genetic Variability ◽  
Morphological Variability ◽  
Fruit Color ◽  
Great Similarity ◽  
Fruit Characteristics ◽  
Family Based ◽  
Cucurbitaceae Family

Species from Cucurbitaceae family are not widely present in Serbia, although because of their morphological and decorative features deserve more attention. The aim of this paper was to study the morphological variability and usage of ten species of the Cucurbiataceae family. Based on genetic variability, species were grouped into 8 clusters. Fruit characteristics of most investigated species showed great similarity and the greatest differences were attained for flower and fruit characteristics. The longest was the fruit of Trichosanthes cucumerina (46.2 cm), while the shortest of Cucumis myriocarpus (3.4 cm) which had the smallest circumference as well (4.4 cm). The largest circumference of fruit was recorded for the species Cucumis aculeatus (16.4 cm). In terms of fruit color Momordica balsamina had a red fruit, allocated from other species whose fruits were in various shades of green. Variability is reflected in large variations in size, shape and color of fruit. Considerating that usages of these species are multiple (food for humans and animals, ornamental) studyed species deserve special attention in their further propagation and use.

scholarly journals First molecular identification of wheat seed gall nematode Anguina tritici races parasitized on wheat in Iraq

2019 ◽  
Vol 11 (1) ◽  
pp. 5-15
Author(s):  
Sulaiman Naif Ami ◽  
Ibrahim Esa Taher ◽  
Fenik Sherzad Hussen ◽  
Ayoub Ibrahim Ahmed
Keyword(s):  
Molecular Identification ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Nematode Species ◽  
Agarose Gel ◽  
Wheat Cultivars ◽  
Wheat Seed ◽  
Kurdistan Region ◽  
Two Cities ◽  
Template Dna

Abstract This study was conducted to identify races of wheat seed gall nematode Anguina tritici by using molecular identification for this nematode species parasitized on durum and bread wheat cultivars. Wheat seed galls of both cultivars were collected from two cities, Erbil and Duhok, from the Kurdistan Region and Iraq respectively. DNA was extracted from both nematode isolates (populations), and then PCR reactions were performed with Internal Transcribed Spacer (ITS) region using primers TW81/AB28 with 2 µl of template DNA of A. tritici for both nematode isolates on both wheat cultivars. The bands of both amplification products of PCR reactions were visualized in position about 800 bp on agarose gel, which indicates that both isolates of A. tritici belong to the same race. On the other hand, no nucleotide differences were observed between the two nematode isolations, as revealed by the sequence alignment of DNA of the internal transcribed spacer (ITS) region and phylogenetic tree, which emphasizes at the same time that both nematode isolates are of the same genetic structure or have the same identity and confirms their belonging to the same nematode race.

scholarly journals Mitochondrial gene cytochrome c oxidase I (CO1) used for molecular identification of Bactrocera zonata in Pakistan

2019 ◽  
Vol 65 (2) ◽  
pp. 82 ◽  
Author(s):  
Ikrma Amad ◽  
Faisal Hafeez ◽  
Muhammad Asaf Khan ◽  
Nazia Nahid ◽  
Muhammad Rizwan Javed ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Molecular Identification ◽  
Mitochondrial Gene ◽  
Cytochrome C Oxidase I ◽  
Bactrocera Zonata

scholarly journals Molecular Identification of some Indian Muscid flies (Diptera: Muscidae) based on mitochondrial gene COII

2017 ◽  
Author(s):  
Devinder Singh ◽  
Ramandeep Achint
Keyword(s):  
Molecular Identification ◽  
Nucleotide Diversity ◽  
Diagnostic Marker ◽  
Mitochondrial Gene ◽  
Haplotype Diversity ◽  
Morphological Characters ◽  
Genetic Distances ◽  
Veterinary Importance ◽  
Coii Gene ◽  
Intraspecific Distance

ABSTRACTTaxonomic identification of flies having medical and veterinary importance is often complicated due to the lookalike morphological characters. Molecular identification of five Indian muscid flies (Musca domestica, Musca sorbens, Musca crassirostris, Stomoxys calcitrans and Haematobia irritans) has been attempted on the basis of mitochondrial COII gene. Sequences of 500-520 bp were analysed and found to be A+T rich. Rate of transitions was higher than transversions. The average haplotype diversity was 0.833 and nucleotide diversity was 0.02547 within the different species, which were calculated with the DnaSP Version 5.0. The genetic distances calculated by K2P model, showed the interspecific distances range more than 8.2%, while the intraspecific distance range had not exceed 0.8%. The 1000 bootstrapped Neighbour-joining tree and Maximum likelihood tree were constructed to establish the phylogenetic relationship between the different muscid species. The results show the robustness of COII gene as a diagnostic marker. The data obtained from this study would be worthy for medical and veterinary entomologists for precise identification of imperative muscid species.

scholarly journals Molecular identification of six species of Calliphoridae (Diptera) with forensic interest in Bogotá, Colombia

2017 ◽  
Vol 57 (12) ◽  
pp. 141
Author(s):  
Angela Sabrina Marquez-Acero ◽  
Juan Manuel Vidal-Garcia ◽  
Luis Francisco Becerra Galindo ◽  
Alexander García García
Keyword(s):  
Maximum Likelihood ◽  
Molecular Identification ◽  
Mitochondrial Gene ◽  
Genetic Distances ◽  
The Body ◽  
Calliphora Vicina ◽  
Taxonomic Identification ◽  
The Family ◽  
Taxonomic Organization ◽  
Likelihood Model

Taxonomic identification of the species involved in the processes of cadaveric decomposition is a fundamental procedure in forensic entomological analysis. Among the species involved in the processes of decay, those of the Calliphoridae family are particularly important because they come to the body in the early stages of decomposition. The aim of this research is to identify six species of Calliphoridae (Calliphora nigribasis, Calliphora vicina, Compsomyopsis verena, Sarconesiopsis magellanica, Chrysomia albiceps and Roraimomusca roraima) with forensic interest found in Bogotá. For that, sequences of 599 bp from mitochondrial gene COII wereas obtained. The identification was made by analysis of genetic distances under Jukes-Cantor model. The results showed levels of interspecific distances greater than 3.7%, while intraspecifics levels does not exceed 2.3%. The genetic distances obtained were used to construct a phenogram under the Maximum Likelihood model and the topology of that tree agrees with the current taxonomic organization for the family Calliphoridae family.

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