CONDITION AND STRUCTURAL ORGANIZATION OF THE GLANDULAR AREA MUCOUS MEMBDANE OF ALBINO RAT HARD PALATE UNDER THE 30-DAY-LONG EFFECT OF ACRYLIC MONOMER

In groups of Wistar rats 3 and 12 months of age, colchicine and radioautography were used to assess mitotic activity in oral epitheliums. Apparent mitotic activity in the epitheliums of the cheek, hard palate, and central, intermediate, and lateral zones of the soft palate depended on the method used and the age of the rat.

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THE EFFECT OF MONOMER OF REMOVABLE DENTURE BASE RESIN ON THE STRUCTURAL ORGANIZATION OF THE GLANDULAR ZONE OF ALBINO RAT HARD PALATE IN THE EXPERIMENT

Wiadomości Lekarskie ◽

10.36740/wlek202012120 ◽

2020 ◽

Vol 73 (12) ◽

pp. 2667-2671

Author(s):

Yevhenii S. Khilinich ◽

Vadym Yu. Davydenko ◽

Ivan І. Starchenko ◽

Mykhailo Ya. Nidzelskyi ◽

Hanna М. Davydenko ◽

...

Keyword(s):

Salivary Glands ◽

Mucous Membrane ◽

Hard Palate ◽

Structural Organization ◽

Experimental Studies ◽

Basal Layer ◽

Acrylic Resin ◽

Albino Rats ◽

Denture Base ◽

Removable Denture

The aim: The paper presents the findings of the study of the 3 month-long effect of the monomer of the “Ftorax” denture base acrylic resin on the structural organization of the mucous membrane and the condition of the salivary glands of the albino rats’ hard palate during the experiment. Materials and methods:To achieve this goal, experimental studies involved mature rats, whose hard palate mucosa was smeared with 2% aqueous solution of the monomer of the “Ftorax” denture base acrylic resin twice a day in the morning and evening. The animals were sacrificed on day 30 of the experiment and following 3 months. Results: The findings of the studies of the structural organization of the mucous membrane and the analysis of the micropreparations of the mucous membrane of the hard palate of the animals of Group III have revealed substantial pathological changes both in the covering epithelium and in the lamina propria. The comparison between the intact animals and group of animals subjected to the 3 month-long effect of the monomer showed a significant thickening of the epithelial layer; a decrease in the mitotic index in the basal layer; impaired stratification of the cellular elements of the spinous layer; manifestations of exudative purulent inflammation, cystic and sclerotic changes in the excretory ducts; increase in the amount of connective tissue in the lobules of the salivary glands; decrease in the volume of the secretory parenchyma. Conclusions: Based on the findings of the study we can conclude that the prolonged effect of the monomer of the denture base acrylic resins leads to disorder of the structural organization of the glandular zone of the hard palate; in its submucous layer the total volume of the salivary glands decreases, which, in turn, significantly reduces secretion and leads to hyposalivation.

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Structural organization of escherichia coli ribosomes as determined by immuno electron microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100081693 ◽

1978 ◽

Vol 36 (2) ◽

pp. 166-167 ◽

Cited By ~ 1

Author(s):

G. Stöffler ◽

R.W. Bald ◽

J. Dieckhoff ◽

H. Eckhard ◽

R. Lührmann ◽

...

Keyword(s):

Electron Microscopy ◽

Escherichia Coli ◽

Ribosomal Proteins ◽

Structural Organization ◽

Three Dimensional ◽

Ribosomal Subunit ◽

Spatial Arrangement ◽

Small Subunit ◽

Antibody Binding ◽

Immuno Electron Microscopy

A central step towards an understanding of the structure and function of the Escherichia coli ribosome, a large multicomponent assembly, is the elucidation of the spatial arrangement of its 54 proteins and its three rRNA molecules. The structural organization of ribosomal components has been investigated by a number of experimental approaches. Specific antibodies directed against each of the 54 ribosomal proteins of Escherichia coli have been performed to examine antibody-subunit complexes by electron microscopy. The position of the bound antibody, specific for a particular protein, can be determined; it indicates the location of the corresponding protein on the ribosomal surface.The three-dimensional distribution of each of the 21 small subunit proteins on the ribosomal surface has been determined by immuno electron microscopy: the 21 proteins have been found exposed with altogether 43 antibody binding sites. Each one of 12 proteins showed antibody binding at remote positions on the subunit surface, indicating highly extended conformations of the proteins concerned within the 30S ribosomal subunit; the remaining proteins are, however, not necessarily globular in shape (Fig. 1).

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Ribosome Structural Organization

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051670 ◽

1974 ◽

Vol 32 ◽

pp. 332-333

Author(s):

James A. Lake

Keyword(s):

Ribosomal Proteins ◽

Structural Organization ◽

Three Dimensional ◽

Small Subunit ◽

Structural Studies ◽

X Ray Diffraction ◽

Specific Antibodies ◽

X Ray ◽

E Coli ◽

Complete Sequences

The understanding of ribosome structure has advanced considerably in the last several years. Biochemists have characterized the constituent proteins and rRNA's of ribosomes. Complete sequences have been determined for some ribosomal proteins and specific antibodies have been prepared against all E. coli small subunit proteins. In addition, a number of naturally occuring systems of three dimensional ribosome crystals which are suitable for structural studies have been observed in eukaryotes. Although the crystals are, in general, too small for X-ray diffraction, their size is ideal for electron microscopy.

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Study of the Molecular Architecture of Keratin Filaments by Electron Microscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053310 ◽

1982 ◽

Vol 40 ◽

pp. 118-119

Author(s):

U. Aebi ◽

P. Rew ◽

T.-T. Sun

Keyword(s):

Electron Microscopy ◽

Structural Organization ◽

Cell Types ◽

Structural Features ◽

Molecular Architecture ◽

The Past ◽

Keratin Filaments ◽

Different Types ◽

10 Nm Filaments ◽

Different Cell Types

Various types of intermediate-sized (10-nm) filaments have been found and described in many different cell types during the past few years. Despite the differences in the chemical composition among the different types of filaments, they all yield common structural features: they are usually up to several microns long and have a diameter of 7 to 10 nm; there is evidence that they are made of several 2 to 3.5 nm wide protofilaments which are helically wound around each other; the secondary structure of the polypeptides constituting the filaments is rich in ∞-helix. However a detailed description of their structural organization is lacking to date.

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The ultrastructure of acinar cells in the external orbital gland of young and old male rats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100082248 ◽

1978 ◽

Vol 36 (2) ◽

pp. 276-277

Author(s):

R. Carriere

Keyword(s):

Young Adulthood ◽

Acinar Cells ◽

Cell Number ◽

Male Rats ◽

Polyploid Cell ◽

Secretory Cells ◽

Age Changes ◽

Albino Rat ◽

Golgi Membranes ◽

Diploid Cells

The external orbital gland of the albino rat exhibits both sexual dimorphism and histological age changes. In males, many cells attain a remarkable degree of polyploidy and an increase of polyploid cell number constitutes the major age change until young adulthood. The acini of young adults have a small lumen and are composed of tall serous cells. Subsequently, many acini acquire a larger lumen with an irregular outline while numerous vacuoles accumulate throughout the secretory cells. At the same time, vesicular acini with a large lumen surrounded by pale-staining low cuboidal diploid cells begin to appear and their number increases throughout old age. The fine structure of external orbital glands from both sexes has been explored and in considering acinar cells from males, emphasis was given to the form of the Golgi membranes and to nuclear infoldings of cytoplasmic constituents.

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