Production and biochemical characterization of xylanases synthesized by the thermophilic fungus Rasamsonia emersonii S10 by solid-state cultivation

The xylanolytic enzyme complex hydrolyzes xylan, and these enzymes have various industrial applications. The goal of this work was to characterize the endoxylanases produced by the thermophilic fungus Rasamsonia emersonii in solid-state cultivation. Tests were carried out to evaluate the effects of pH, temperature, glycerol and phenolic compounds on enzyme activity. Thermal denaturation of one isolated enzyme was evaluated. The crude extract from R. emersonii was applied to breakdown pretreated sugarcane bagasse, by quantifying the release of xylose and glucose. The optimum pH value for the crude enzymatic extract was 5.5, and 80 �C was the optimum temperature. Regarding the stability of the crude extract, the highest values occurred between the pH ranges from 4 to 5.5. Several phenolic compounds were tested, showing an increase in enzymatic activity on the crude extract, except for tannic acid. Zymography displayed four corresponding endoxylanase bands, which were isolated by extraction from a polyacrylamide gel. The thermodynamic parameters of isolated Xylanase C were evaluated, showing a half-life greater than 6 h at 80 �C (optimum temperature), in addition to high melting temperature (93.3 �C) and structural resistance to thermal denaturation. Pretreated sugarcane bagasse breakdown by the crude enzymatic extract from R. emersonii has good hemicellulose conversion to xylose.

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Production of cellulases by solid state fermentation using natural and pretreated sugarcane bagasse with different fungi

Biocatalysis and Agricultural Biotechnology ◽

10.1016/j.bcab.2018.10.019 ◽

2019 ◽

Vol 17 ◽

pp. 1-6 ◽

Cited By ~ 14

Author(s):

Gabriella Soares Borges Salomão ◽

Jacyele Clarindo Agnezi ◽

Larissa Bastos Paulino ◽

Luana Borchardt Hencker ◽

Taísa Shimosakai de Lira ◽

...

Keyword(s):

Solid State ◽

Sugarcane Bagasse ◽

Solid State Fermentation ◽

Pretreated Sugarcane Bagasse

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Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse

Semina Ciências Agrárias ◽

10.5433/1679-0359.2020v41n5supl1p2097 ◽

2020 ◽

pp. 2097-2116 ◽

Cited By ~ 1

Author(s):

Priscila Aparecida Casciatori Frassatto ◽

Fernanda Perpétua Casciatori ◽

João Cláudio Thoméo ◽

Eleni Gomes ◽

Maurício Boscolo ◽

...

Keyword(s):

Solid State ◽

Sugarcane Bagasse ◽

Enzyme Production ◽

Packed Bed ◽

Aeration Rate ◽

Hydrolysis Time ◽

Enzymatic Extract ◽

Packed Bed Bioreactor ◽

High Concentrations ◽

Hydrolysis Of

Cellulases are essential for the hydrolysis of lignocellulosic materials in the production of second generation ethanol. Solid-state cultivation is a process that provides high concentrations of enzymes that can be used in this hydrolysis. The objectives of this work were to produce cellulases by cultivating the fungus Myceliophthora thermophila I-1D3b in a packed bed bioreactor with sugarcane bagasse (SCB) and wheat bran (WB) as substrate and to evaluate the efficiency of the enzymatic extract in the hydrolysis of SCB in natura (BIN) and pretreated with ozone, alkali and ultrasound (BOU). The conditions for enzyme production in the bioreactor were SCB:WB at a ratio of 2.3:1 (w/w), 75 % moisture content; 45 ºC; aeration rate 240 L h-1 and 96 h. The enzyme production was evaluated by endoglucanase, xylanase, filter paper (FPU) and ?-glycosidase activities. For the application of the enzymes, a central composed response surface design with 5 repetitions of the central point was used, taking enzyme volume and hydrolysis time as factors. Such cultivation yielded the following enzymatic activities: 723 U gss-1 of endoglucanases, 2024 U gss-1 of xylanase, 12.6 U gss-1 of FPU and 41 U gss-1 of ?-glucosidase. The results of the application tests indicated the best conditions as 7.0 ml of the enzyme extract (4.2 FPU) and 6 hours for BIN and BOU. The best cellulose-glucose conversions were obtained for BOU, reaching 32.1 % at 65 ºC. In conclusion, the enzyme production in the packed bed bioreactor was efficient and BOU pretreatment improved the hydrolysis of biomass, increasing the efficiency of conversion of cellulose to glucose.

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Solid-state bioprocessing of sugarcane bagasse with Auricularia fuscosuccinea for phenolic compounds extraction

Preparative Biochemistry & Biotechnology ◽

10.1080/10826068.2021.1986722 ◽

2021 ◽

pp. 1-10

Author(s):

Romina Coniglio ◽

Gabriela Díaz ◽

Cinthya López ◽

María Restelli ◽

Emanuel Grassi ◽

...

Keyword(s):

Solid State ◽

Phenolic Compounds ◽

Sugarcane Bagasse

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Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse

Industrial Crops and Products ◽

10.1016/j.indcrop.2018.05.022 ◽

2018 ◽

Vol 122 ◽

pp. 66-75 ◽

Cited By ~ 24

Author(s):

Natália Paganini Marques ◽

Josiani de Cassia Pereira ◽

Eleni Gomes ◽

Roberto da Silva ◽

Angela Regina Araújo ◽

...

Keyword(s):

Solid State ◽

Sugarcane Bagasse ◽

Endophytic Fungi ◽

Solid State Fermentation ◽

Enzymatic Saccharification ◽

Lignocellulosic Substrates ◽

Pretreated Sugarcane Bagasse

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Effect of phenolic compounds from pretreated sugarcane bagasse on cellulolytic and hemicellulolytic activities

Bioresource Technology ◽

10.1016/j.biortech.2015.08.120 ◽

2016 ◽

Vol 199 ◽

pp. 275-278 ◽

Cited By ~ 51

Author(s):

Michele Michelin ◽

Eduardo Ximenes ◽

Maria de Lourdes Teixeira de Moraes Polizeli ◽

Michael R. Ladisch

Keyword(s):

Phenolic Compounds ◽

Sugarcane Bagasse ◽

Pretreated Sugarcane Bagasse

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Application of Response Surface Methodology for Optimization of Mushroom Saccharification using Crude Enzymatic Extract from Solid-State Fermentation of Pineapple Peels by Aspergillus niger KWM

10.21203/rs.3.rs-442666/v1 ◽

2021 ◽

Author(s):

Daniel Otieno Ojwang ◽

Mulaa F. Jakim ◽

George Obiero ◽

Jacob Midiwo

Keyword(s):

Response Surface Methodology ◽

Solid State ◽

Response Surface ◽

Solid State Fermentation ◽

Crude Extract ◽

Low Cost ◽

Coefficient Of Determination ◽

Enzymatic Extract ◽

High Yields ◽

Cellular Components

Abstract Mushrooms are a rich source of high value compounds. Efficient enzymatic degradation of mushroom cell-wall matrix is therefore critical in the recovery of cellular components in high yields. In the present study, the effect of reaction variables on mushroom saccharification using crude enzymatic extract was evaluated and optimized using the central composite design based on the response surface methodology. The crude extract displayed CMCase, Fpase and xylanase activities of 1.23Umg− 1 protein, 0.95Umg− 1 protein and 1.52 Umg− 1 protein. The model was validated by the analysis of variance with a coefficient of determination of 0.866 and F test value of 7.39, making the model valid at the 95 % confidence limit. The model achieved glucose yield of 1.490mg/mL at pH 6.5, temperature 50oC, enzyme load of 5% (v/v) and reaction time of 12h. The experiment using optimal model conditions yielded 1.582 mg/mL glucose which is 1.1 folds higher than the predicted model value. This study demonstrated potential of crude extract from solid-state fermentation of low-cost pineapple peels in mushroom processing.

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