Effect of phenolic compounds from pretreated sugarcane bagasse on cellulolytic and hemicellulolytic activities

The xylanolytic enzyme complex hydrolyzes xylan, and these enzymes have various industrial applications. The goal of this work was to characterize the endoxylanases produced by the thermophilic fungus Rasamsonia emersonii in solid-state cultivation. Tests were carried out to evaluate the effects of pH, temperature, glycerol and phenolic compounds on enzyme activity. Thermal denaturation of one isolated enzyme was evaluated. The crude extract from R. emersonii was applied to breakdown pretreated sugarcane bagasse, by quantifying the release of xylose and glucose. The optimum pH value for the crude enzymatic extract was 5.5, and 80 �C was the optimum temperature. Regarding the stability of the crude extract, the highest values occurred between the pH ranges from 4 to 5.5. Several phenolic compounds were tested, showing an increase in enzymatic activity on the crude extract, except for tannic acid. Zymography displayed four corresponding endoxylanase bands, which were isolated by extraction from a polyacrylamide gel. The thermodynamic parameters of isolated Xylanase C were evaluated, showing a half-life greater than 6 h at 80 �C (optimum temperature), in addition to high melting temperature (93.3 �C) and structural resistance to thermal denaturation. Pretreated sugarcane bagasse breakdown by the crude enzymatic extract from R. emersonii has good hemicellulose conversion to xylose.

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Influence of the Chemical Composition on the Enzymatic Hydrolysis of Hot Water and Organosolv Pretreated Sugarcane Bagasse

Waste and Biomass Valorization ◽

10.1007/s12649-019-00657-2 ◽

2019 ◽

Vol 11 (7) ◽

pp. 3337-3344

Author(s):

María E. Vallejos ◽

Marcia D. Zambon ◽

María C. Area ◽

Antonio A. S. Curvelo

Keyword(s):

Chemical Composition ◽

Enzymatic Hydrolysis ◽

Sugarcane Bagasse ◽

Hot Water ◽

Pretreated Sugarcane Bagasse ◽

Hydrolysis Of

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Production of cellulases by solid state fermentation using natural and pretreated sugarcane bagasse with different fungi

Biocatalysis and Agricultural Biotechnology ◽

10.1016/j.bcab.2018.10.019 ◽

2019 ◽

Vol 17 ◽

pp. 1-6 ◽

Cited By ~ 14

Author(s):

Gabriella Soares Borges Salomão ◽

Jacyele Clarindo Agnezi ◽

Larissa Bastos Paulino ◽

Luana Borchardt Hencker ◽

Taísa Shimosakai de Lira ◽

...

Keyword(s):

Solid State ◽

Sugarcane Bagasse ◽

Solid State Fermentation ◽

Pretreated Sugarcane Bagasse

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On-site integrated production of cellulases and β-glucosidases by Trichoderma reesei Rut C30 using steam-pretreated sugarcane bagasse

10.1101/461012 ◽

2018 ◽

Cited By ~ 3

Author(s):

Marcella Fernandes de Souza ◽

Elba Pinto da Silva Bon ◽

Ayla Sant’ Ana da Silvab

Keyword(s):

Carbon Source ◽

Lignocellulosic Biomass ◽

Trichoderma Reesei ◽

Sugarcane Bagasse ◽

Enzyme Preparation ◽

Integrated Approach ◽

Aspergillus Awamori ◽

Pretreated Sugarcane Bagasse ◽

Hydrolysis Of ◽

Rut C30

AbstractThe high cost of commercial cellulases still hampers the economic competitiveness of the production of fuels and chemicals from lignocellulosic biomasses. This cost may be decreased by the on-site production of cellulases with the integrated use of the lignocellulosic biomass as carbon source. This integrated approach was evaluated in the present study whereby steam-pretreated sugarcane bagasse (SPSB) was used as carbon source for the production of cellulases by Trichoderma reesei Rut C30 and the produced enzymes were subsequently used for SPSB hydrolysis. An enzyme preparation with a high cellulase activity, of 1.93 FPU/mL, was obtained, and a significant β-glucosidase activity was achieved in buffered media, indicating the importance of pH control during enzyme production. The hydrolysis of SPSB with the laboratory-made mixture resulted in a glucose yield of 80%, which was equivalent to those observed for control experiments using commercial enzymes. Even though the supplementation of this mixture with external β-glucosidase from Aspergillus awamori was found to increase the initial hydrolysis rates, it had no impact on the final hydrolysis yield. It was shown that SPSB is a promising carbon source for the production of cellulases and β-glucosidases by T. reesei Rut C30 and that the enzyme preparation obtained is effective for the hydrolysis of SPSB, supporting the on-site integrated approach to decrease the cost of the enzymatic hydrolysis of lignocellulosic biomass.

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