scholarly journals KAJIAN LITERATUR DAN KROMATOGRAFI UNTUK HERBA KESUM

2020 ◽  
Vol 7 (2) ◽  
pp. 49
Author(s):  
Ibtisam Abdul Wahab ◽  
Nor Amlizan Ramli ◽  
Hannis Fadzillah Mohsin ◽  
Kathleen Jalani
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Literature Search ◽  
Chemical Substance ◽  
Biochemical Data ◽  
Future Directions ◽  
Diterpene Alkaloids ◽  
Medicinal Compound ◽  
Root Extracts ◽  
Liquid Chromatographic

<p align="center"><strong>ABSTRAK</strong></p><p> </p><p>Spesis <em>Polygonum</em> (misalnya <em>P. aviculare</em>) dilaporkan mempunyai kandungan alkaloid. Begitu juga dengan daun kesum (<em>P. minus</em> Huds), yang terbukti sebagai sumber molekul perubatan semulajadi. Masalah yang diidentifikasi dari kajian kesum termasuklah di dalam aspek fitokimia genus <em>Polygonum</em> ini, yang belum pernah disiasat secara mendalam. Oleh itu, sasaran kajian ini adalah untuk mengulas data biokimia tumbuhan tersebut melalui metode literatur serta menjalankan telaah analisis kimia terhadap ekstrak kesum. Dari artikel dan jurnal penerbitan, bahagian daun dan akar dipelajari melalui kaedah kromatografi kolom. Tambahan itu, sebatian kimia dari kesum dicirikan dengan teknik spektroskopi resonans magnetik nuklear. Temuan signifikan diperolehi apabila senyawa indol dan alkaloid diterpen C20 berjaya dikarakterisasi. Secara kesimpulannya, projek di masa hadapan boleh ditumpu kepada ujikaji antikanser dan anti-penuaan dari herba kesum ini.   </p><p> </p><p><strong>Kata kunci</strong>  : herba, kesum, literatur, <em>Polygonum</em></p><p> </p><p align="center"><strong><em>ABSTRACT</em></strong></p><p> </p><p><em>Polygonum species (e.g. P. aviculare) were reported to consist of alkaloidal nature. Likewise, the kesum leaves (P. minus Huds) is </em><em>proven as a source of natural medicinal compound.  </em><em>The identified problem in the kesum research would include the phytochemistry </em><em>aspects of this Polygonum </em><em>genus, which is not yet thoroughly investigated</em><em>, via literature search.  </em><em>Therefore, the aim of this study is to accumulate </em><em>and review the biochemical data of this herb. </em><em>From the articles and published journals, the leaves and root extracts</em><em> were analysed by using liquid chromatographic </em><em>technique. In addition, the chemical substance from kesum could be elucidated via nuclear magnetic resonance spectroscop</em><em>y. The </em><em>significant findings could be obtained when the indole and and C20-diterpene alkaloids</em><em> were successfully characterised. In summary, future directions of the project </em><em>could </em><em>focus on anticancer</em><em> and anti-aging experiments of this kesum herb. </em></p><p> </p><p><strong><em>Keywords</em></strong><em>     : herb, kesum, literatur</em><em>e, Polygonum </em></p>

Gas—Liquid Chromatographic Measurement of Guanidino Acids

1975 ◽  
Vol 21 (7) ◽  
pp. 838-843 ◽  
Author(s):  
Harini Patel ◽  
Burton D Cohen
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Liquid Chromatography ◽  
Liquid Phase ◽  
Magnetic Resonance ◽  
Gas Liquid Chromatography ◽  
Butyl Esters ◽  
Silicone Liquid ◽  
Chromatographic Measurement ◽  
Liquid Chromatographic

Abstract We present a method for separating and measuring guanidino acids by gas—liquid chromatography. Compared to ion-exchange techniques, this system is faster, more sensitive, requires smaller sample volumes, is independent of colorimetric reactions, and permits simultaneous determination of both amino and guanidino acids. N-Trifluoroacetyl-n-butyl esters are formed and then are separated by using a column containing a mixed silicone liquid phase coated on Chromosorb W-HP. Analytical recoveries from plasma ranged from 86 to 112% and were optimal when purification and derivatization were done without prior protein precipitation. Speculations as to the cause of this interference by protein include coprecipitation, protein-binding, and cyclization of the guanidines in the acids used for denaturation. Evidence presented suggests that all three occur: ultrafiltrability is diminished in the presence of protein and nuclear magnetic resonance demonstrates cyclization of some of these esters.

scholarly journals 1H Nuclear Magnetic Resonance: A Future Approach to the Metabolic Profiling of Psychedelics in Human Biofluids?

2021 ◽  
Vol 12 ◽  
Author(s):  
Sylvana Vilca-Melendez ◽  
Malin V. Uthaug ◽  
Julian L. Griffin
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Metabolic Profiling ◽  
Proton Nuclear Magnetic Resonance ◽  
Future Directions ◽  
H Nmr ◽  
Nuclear Magnetic ◽  
Psychedelic Research

While psychedelics may have therapeutic potential for treating mental health disorders such as depression, further research is needed to better understand their biological effects and mechanisms of action when considering the development of future novel therapy approaches. Psychedelic research could potentially benefit from the integration of metabonomics by proton nuclear magnetic resonance (1H NMR) spectroscopy which is an analytical chemistry-based approach that can measure the breakdown of drugs into their metabolites and their metabolic consequences from various biofluids. We have performed a systematic review with the primary aim of exploring published literature where 1H NMR analysed psychedelic substances including psilocin, lysergic acid diethylamide (LSD), LSD derivatives, N,N-dimethyltryptamine (DMT), 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) and bufotenin. The second aim was to assess the benefits and limitations of 1H NMR spectroscopy-based metabolomics as a tool in psychedelic research and the final aim was to explore potential future directions. We found that the most current use of 1H NMR in psychedelic research has been for the structural elucidation and analytical characterisation of psychedelic molecules and that no papers used 1H NMR in the metabolic profiling of biofluids, thus exposing a current research gap and the underuse of 1H NMR. The efficacy of 1H NMR spectroscopy was also compared to mass spectrometry, where both metabonomics techniques have previously shown to be appropriate for biofluid analysis in other applications. Additionally, potential future directions for psychedelic research were identified as real-time NMR, in vivo1H nuclear magnetic resonance spectroscopy (MRS) and 1H NMR studies of the gut microbiome. Further psychedelic studies need to be conducted that incorporate the use of 1H NMR spectroscopy in the analysis of metabolites both in the peripheral biofluids and in vivo to determine whether it will be an effective future approach for clinical and naturalistic research.

Gas-Liquid Chromatographic Derivatization and Chromatography of N-Methylcarbamate Methoxy Derivatives Formed with Trimethylanilinium Hydroxide

1982 ◽  
Vol 65 (6) ◽  
pp. 1452-1456
Author(s):  
Leonard Ogierman
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Spectral Analysis ◽  
Magnetic Resonance ◽  
Infrared Absorption Spectroscopy ◽  
Mass Spectral Analysis ◽  
Liquid Chromatograph ◽  
Mass Spectral ◽  
Carbamate Insecticides ◽  
Synthetic Standard ◽  
Liquid Chromatographic

Abstract Carbamate insecticides injected into a gas-liquid chromatograph react with trimethylanilinium hydroxide to give derivatives that have good gas-liquid chromatographic properties. The substituted phenyl N-methylcarbamates give methoxy derivatives by this procedure. Chromatographic response was linear with increased concentration for the synthetic standard and the on-column methylation products of the examined substances. The standards obtained were identified by ultraviolet and infrared absorption spectroscopy, as well as by nuclear magnetic resonance and mass spectral analysis.

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