scholarly journals Use of Carazolol at Pre-Synchronized Timed Artificial Insemination in Cows

2008 ◽  
Vol 77 (1) ◽  
pp. 59-64
Author(s):  
Ş. M. Pancarci ◽  
Y. Öztürkler ◽  
Ö. Güngör ◽  
C. Kaçar ◽  
S. Yildiz ◽  
...  
Keyword(s):  
Beneficial Effect ◽  
Corpus Luteum ◽  
Artificial Insemination ◽  
Adrenergic Receptor ◽  
Jugular Vein ◽  
Receptor Blocker ◽  
Transrectal Ultrasonography ◽  
Physiologic Saline ◽  
Timed Artificial Insemination ◽  
Rectal Palpation

Efficacy of the β-adrenergic receptor blocker (carazolol) at Timed Artificial Insemination (TAI) was investigated. Cows (n = 73) were pre-synchronized with two PGF2α injections given 14 d apart to initiate the Ovsynch protocol at early and middle luteal stages 14 d later, and received injections of GnRH and PGF2α seven d apart followed by GnRH 48 h later, and TAI 16 - 18 h later. Corpus luteum (CL) was detected via rectal palpation at the beginning of the Ovsynch protocol. Carazolol (Treatment I; n = 41) or physiologic saline (Treatment II; n = 32) were administered via jugular vein five min before TAI. Uterine tone was determined prior to infusion and at TAI via rectal palpation. Pregnancies were diagnosed with transrectal ultrasonography 40 ± 7 d after TAI. Uterine tone was 2.8 (1.4 - 5.3) times higher (P < 0.01) in Treatment I than that in Treatment II at TAI. Increase in uterine tone was affected by treatment × CL × parity (P < 0.05), and 66.7%, 75%, 52.6%, 100% and 16.7%, 33.3%, 25%, 28.6% in primiparous cows with CL and without CL, and in multiparous cows with CL and without CL in Treatments I and II, respectively. Pregnancy rates did not differ between treatments I (34.2%) and II (40.6%). In conclusion, no beneficial effect of carazolol administration prior to TAI was found except for the increased uterine tone.

scholarly journals The ovulatory response to human chorionic gonadotropin administration on day 4 post timed artificial insemination improved fertility in repeat breeder cows

2021 ◽  
Vol 90 (1) ◽  
pp. 15-20
Author(s):  
Gulnaz Yilmazbas-Mecitoglu ◽  
Ahmet Gumen ◽  
Ebru Karakaya-Bilen ◽  
Abdulkadir Keskin ◽  
Baris Guner ◽  
...  
Keyword(s):  
Beneficial Effect ◽  
Dairy Cows ◽  
Corpus Luteum ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Artificial Insemination ◽  
Pregnancy Rates ◽  
Control Group ◽  
Timed Artificial Insemination ◽  
Repeat Breeder

This study focused on determining the success of inducing endogenous progesterone source (by formation of accessory corpus luteum) by human chorionic gonadotropin (hCG) administration on day 4 post timed artificial insemination (TAI) and on evaluating the effect of the day of hCG administration (day 4 or 5 post TAI) on pregnancy rates in repeat breeder cows according to their responses to hCG administrations. Non-pregnant cyclic lactating Holstein dairy cows with more than three services despite no clinical reproductive abnormalities were enrolled in the study. All cows (n = 192) received the Ovsynch protocol (GnRH-7d-PGF2α-56h-GnRH-16/18h-TAI) and hCG administration (1500 IU, i.m.) was performed in group hCG4 (n = 64) on day 4 and in group hCG5 (n = 68) on day 5 post TAI. The control group (CON, n = 60) did not receive any hCG treatment. The responses (ovulation rate) to hCG (~64%) were similar on days 4 and 5 post TAI. Pregnancy per AI (P/AI) at 31 days was similar among the groups (48.3% in CON, 48.4% in hCG4, and 42.6% in hCG5). Regardless of the day of the treatment (hCG4 + hCG5), the cows had an ovulatory response to hCG (54.1%), and a greater P/AI (P = 0.007) at 31 days than those without a response (29.8%). The administration of hCG on either day 4 or 5 post TAI did not increase overall pregnancy rates, however, the ovulatory response to the post TAI hCG administration on day 4 improved fertility in repeat breeder cows. Further studies are needed with a larger number of animals to reveal this potentiallly beneficial effect.

scholarly journals Efficacy of hCG and GnRH with Respect to Follicular Size and Presence of the Corpus Luteum in Cosynch Protocol Integrated with Norgestomet in Lactating Cows

2013 ◽  
Vol 57 (1) ◽  
pp. 61-64 ◽  
Author(s):  
Şükrü Metіn Pancarci ◽  
Necdet Cankat Lehіmcіoğlu ◽  
Umut Çağin Ari ◽  
Örsan Güngör ◽  
Özay Akbulut
Keyword(s):  
Corpus Luteum ◽  
Artificial Insemination ◽  
Transrectal Ultrasonography ◽  
Preovulatory Follicle ◽  
Lactating Cows ◽  
Follicular Size ◽  
Timed Artificial Insemination

AbstractEfficacies of hCG and GnRH with respect to follicular diameter in Cosynch protocol were investigated. Lactating cows (n=119) received injections of GnRH and PGF2 7 d apart. Norgestomet ear implant was inserted at GnRH administration and removed prior to PGF2 injections. Presence of the corpus luteum (CL) at GnRH and PGF2 injections, and diameter of preovulatory follicle [small (SPF) <8 mm or large (LPF) ≥8 mm] at PGF2 were determined with transrectal ultrasonography (USG). The animals were randomly treated with GnRH (0.01 mg; GPG, n=62) or hCG (1,500 IU; GPH, n=57) at timed artificial insemination (TAI) 56 h after injection of PGF2α. Pregnancies were diagnosed with USG 32-35 d after TAI. Pregnancies per TAI (P/TAI) were significantly (P<0.05) lower in GPH cows (28.1%; 16/57) compared to those in GPG animals (46.8%; 29/62). P/TAI with respect to follicular size did not differ between GPG and GPH cows, although P/TAI was numerically higher in LPF (30.0%; 15/50) than in SPF (14.3%; 1/7) in GPH cows. P/TAI in cows without CL at PGF2 was 2.5 (1.0-6.7) times higher (P<0.01) in GPG (20.0%; 3/15) compared to GPH (10.5%; 2/19). In conclusion, use of hCG in milieu of the second GnRH in Cosynch+progesterone insert protocol did not have any advantage in lactating cows.

scholarly journals Study on the effect of insemination time on pregnancy rate of Bangladeshi buffalo in intensive farming

2019 ◽  
Vol 16 (2) ◽  
pp. 143-152
Author(s):  
MA Hamid
Keyword(s):  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Conception Rate ◽  
Transrectal Ultrasonography ◽  
Livestock Breeding ◽  
Intensive Farming ◽  
Rectal Palpation

The study was designed to evaluate the insemination time and pregnancy rate of Bangladeshi buffalo at Lal Teer Livestock Breeding and Research Farm, Bhaluka, Mymensingh, Bangladesh during January 2017 to June 2018. A total of 30 cyclic buffaloes were included in this study. The animals were inseminated under four different times, i.e. inseminated i) between 0-6 hours after seeing first sign of estrous, ii) between 6-12 hours after seeing first sign of estrous, iii) between 12-18 hours after seeing first sign of estrous and v) between 18-24 hours after seeing first sign of estrous. The pregnancies (positive or negative) were confirmed by rectal palpation and transrectal ultrasonography after 60 days of artificial insemination (AI). The total pregnant animals in this study were 40%. The highest pregnancy rate (60%) was observed in insemination between 12-18 hours after seeing first sign of estrous and the lowest pregnancy rate (0%) was observed in insemination between 0-6 hours after seeing first sign of estrous. The findings of the study suggest that, the conception rate of buffalo depend on the time of AI, detection of proper estrus symptoms and site of semen deposition. SAARC J. Agri., 16(2): 143-152 (2018)

scholarly journals Vascular and morphological features of the corpus luteum 12 to 20 days after timed artificial insemination in dairy cattle

2019 ◽  
Vol 102 (6) ◽  
pp. 5612-5622 ◽  
Author(s):  
Luiz G. Siqueira ◽  
Eduardo K. Arashiro ◽  
Alberto M. Ghetti ◽  
Eliza D. Souza ◽  
Luiz F. Feres ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Corpus Luteum ◽  
Artificial Insemination ◽  
Morphological Features ◽  
Timed Artificial Insemination

scholarly journals Corpus Luteum Morphology and Function After Timed Artificial Insemination (TAI) in Nelore Heifers Supplemented with Sunflower Seed in their Diets 

2021 ◽  
Vol 16 (3) ◽  
pp. 121-127
Author(s):  
Gabriel Molinari de Mattos ◽  
Beatriz de Moraes Ropelli ◽  
Claudia Maria Bertan Membrive ◽  
Angélica Leão Baltazar ◽  
Lilian Francisco Arantes de Souza ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Artificial Insemination ◽  
Sunflower Seed ◽  
Timed Artificial Insemination ◽  
And Function

24 EFFECT OF GnRH ON FIXED-TIMED ARTIFICIAL INSEMINATION PREGNANCY RATES OF WHITE-TAILED DEER

2010 ◽  
Vol 22 (1) ◽  
pp. 170
Author(s):  
J. Lambe ◽  
W. Forbes ◽  
B. M. Olcott ◽  
D. E. Sanders ◽  
R. A. Godke ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Pregnancy Rates ◽  
Transrectal Ultrasonography ◽  
Estrus Synchronization ◽  
Control Groups ◽  
Motile Cells ◽  
Timed Artificial Insemination ◽  
And Control ◽  
Uterine Body ◽  
Synchronization Protocol

During the fall 2008 breeding season in Louisiana, 2 synchronization protocols for fixed-timed artificial insemination (FTAI) in White-tailed deer were assessed. The objective was to determine if GnRH at FTAI improved pregnancy rates in White-tailed deer. White-tailed does (n = 35) with a mean body weight of 65.8 kg (range: 54.0 to 79.8 kg) and average age of 5.9 years (range: 1.5 to 9.5 years) were stratified by weight, age, and last fawning date into 2 groups. Treatment and control synchronization protocols were randomly assigned to each group. Does were synchronized with a CIDR-G device for 14 d and were then subjected to either FTAI 60 hpost-CIDR removal (control, n = 17) or 100 μg (i.m) injection of GnRH at FTAI 60 h post-CIDR removal (treated, n = 18). At insemination (AI), insemination pipette placement score (IP; 0 = at cervical os to 3 = within uterine body), mucous scores (clear/cloudy, viscous/nonviscous, or no secretions), vulva assessments (hyperemic/nonhyperemic and swollen/not swollen), and sperm progressive motility were recorded. Does were inseminated with frozen-thawed sperm (5 × 107 progressively motile cells pre-freeze) from 2 fertile bucks stratified across each treatment group. Starting 28 d following AI, intact bucks (ITB) were introduced into both groups for natural mating. Pregnancy was determined via transrectal ultrasonography 73 or 80 d postinsemination. Does, confirmed pregnant via ultrasonography, had fawns within the reported gestation range of 187 to 222 days. Placentomes were visualized and measured in AI pregnancies (range: 32.7 to 56.2 mm in length), whereas pregnancies derived from ITB presented no identifiable placentomes. However, crown-rump measurements were obtainable from ITB pregnancies (range: 13.4 to 21.7 mm). Five does were not included in the final analyses as they were either lost to predators or removed because of illness. IP (1.4 ± 0.24 v. 1.3 ± 0.30; P = 0.671), mucous classifications (3.0 ± 0.35 v. 2.5 ± 0.39; P = 0.311), vulva assessments (2.1 ± 0.29, 1.7 ± 0.23; P = 0.223), and sperm motility (1.6 ± 0.16, 1.7 ± 0.22; P = 0.829) were not different for pregnant and nonpregnant AI does, respectively. Treatment did not affect AI pregnancy rates (53 v. 27%) or fecundity rates (1.6 v. 1.3 offspring/doe) for the GnRH treated and control groups, respectively. Addition of GnRH to a 14-day estrus synchronization protocol did not result in significantly higher pregnancy rates compared with controls. More studies are needed to determine the effect of GnRH on White-tailed pregnancies following FTAI protocols. We have demonstrated that differentiating pregnancies derived from AI and ITB could be accomplished by utilizing transrectal ultrasonography as early as 73 d postinsemination in White-tailed does.

scholarly journals Validation of a novel timed artificial insemination protocol in beef cows with a functional corpus luteum detected by ultrasonography

2018 ◽  
Vol 64 (2) ◽  
pp. 109-115
Author(s):  
Hisashi FUNAKURA ◽  
Ayumi SHIKI ◽  
Yuji TSUBAKISHITA ◽  
Shogo MIDO ◽  
Hiromu KATAMOTO ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Artificial Insemination ◽  
Beef Cows ◽  
Timed Artificial Insemination

New treatments associated with timed artificial insemination in small ruminants

2004 ◽  
Vol 16 (4) ◽  
pp. 403 ◽  
Author(s):  
Alejo Menchaca ◽  
Edgardo Rubianes
Keyword(s):  
Corpus Luteum ◽  
Artificial Insemination ◽  
Production Systems ◽  
Small Ruminants ◽  
Prostaglandin F2α ◽  
Hormonal Control ◽  
Efficient Estimation ◽  
New Information ◽  
Timed Artificial Insemination ◽  
New Treatments

Timed artificial insemination (TAI) is a very important tool in production systems, as it has a direct impact on cost-efficiency by reducing labour resulting from oestrus detection. However, to make TAI commercially feasible, hormonal treatments need to assure acceptable pregnancy rates and be economically viable. Recent advances in the knowledge of ovarian physiology (e.g. determination of follicular waves, sensitivity of the early corpus luteum) in small ruminants allowed the development of new treatments focused on an efficient estimation and synchronisation of the time of ovulation of the females of a treated flock/herd. In this review we summarise new information and concepts in the hormonal control of the life span of the corpus luteum, as well as methods to manipulate follicular growth in small ruminants. Additionally, we elaborate on recent studies concerning the use of TAI associated with short progestogen treatment in goats and the newly developed Synchrovine™ protocol (two doses of prostaglandin F2α given seven days apart) in sheep.

scholarly journals Pregnancy rates and peripheral progesterone levels following Ovsynch or CIDR ovulation synchronization/timed artificial insemination protocols in postpartum dairy cows

2008 ◽  
Vol 88 (3) ◽  
pp. 457-461
Author(s):  
M. Aali ◽  
T. Pretheeban ◽  
G. Giritharan ◽  
R. Rajamahendran
Keyword(s):  
Dairy Cows ◽  
Key Words ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Sampling Period ◽  
Pregnancy Rates ◽  
Key Words Pregnancy ◽  
Milk Samples ◽  
Timed Artificial Insemination ◽  
Rectal Palpation

Peripheral progesterone (P4) concentrations as well as pregnancy rates (PR) between cows treated with Ovsynch or CIDR ovulation synchronization/TAI protocols were compared. Two hundred and twenty-seven postpartum lactating Holstein cows were randomly assigned to Ovsynch (n = 111) or CIDR (n = 116) ovulation synchronization/TAI protocols. Pregnancy was diagnosed by ultrasonography at day 35 after TAI and later confirmed by rectal palpation at day 60 post TAI. Milk samples were taken from the beginning of treatment (day -10) until day 35 after TAI (day 0) for P4 determination. Pregnancy rates for Ovsynch and CIDR groups of cows were 31 and 41%, respectively. The PR of ovulation synchronized cows for Ovsynch and CIDR groups were 49 and 69%, respectively. Progesterone levels during the sampling period were similar (P > 0.05) between Ovsynch and CIDR treated cows. Key words: Pregnancy rate, progesterone, Ovsynch, CIDR, cows

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