Study on the effect of insemination time on pregnancy rate of Bangladeshi buffalo in intensive farming

The study was designed to evaluate the insemination time and pregnancy rate of Bangladeshi buffalo at Lal Teer Livestock Breeding and Research Farm, Bhaluka, Mymensingh, Bangladesh during January 2017 to June 2018. A total of 30 cyclic buffaloes were included in this study. The animals were inseminated under four different times, i.e. inseminated i) between 0-6 hours after seeing first sign of estrous, ii) between 6-12 hours after seeing first sign of estrous, iii) between 12-18 hours after seeing first sign of estrous and v) between 18-24 hours after seeing first sign of estrous. The pregnancies (positive or negative) were confirmed by rectal palpation and transrectal ultrasonography after 60 days of artificial insemination (AI). The total pregnant animals in this study were 40%. The highest pregnancy rate (60%) was observed in insemination between 12-18 hours after seeing first sign of estrous and the lowest pregnancy rate (0%) was observed in insemination between 0-6 hours after seeing first sign of estrous. The findings of the study suggest that, the conception rate of buffalo depend on the time of AI, detection of proper estrus symptoms and site of semen deposition. SAARC J. Agri., 16(2): 143-152 (2018)

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Estrus and pregnancy rate of Simmental-Ongole Crossbred and Ongole Grade heifer after being synchronized and inseminated

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.43.4.438-444 ◽

2018 ◽

Vol 43 (4) ◽

pp. 438

Author(s):

S. Sutiyono ◽

D. Samsudewa ◽

A. Suryawijaya

Keyword(s):

Pregnancy Rate ◽

Artificial Insemination ◽

Reproductive Organs ◽

Estrus Synchronization ◽

Purposive Sampling ◽

Frozen Semen ◽

Research Population ◽

Rectal Palpation

The aim of the study was to examine the synchronization of estrus and artificial insemination in heifers of Ongole grade and Simental-Ongole grade. The research used 19 Ongole Grade (OG) and 18 Simental-Ongole Crossbred (SOC) heifers. This research applied purposive sampling to determine the research population. The criteria to select the population were heifers which have in mature body. The normality of heifer’s reproductive organs were identified by performing rectal palpation, then 50 mg of medroxy progesterone acetate on vaginal sponge was used to synchronized estrus. The estrus heifer was inseminated using frozen semen. The observed parameters were the number of heifers with normal and abnormal reproductive organs, estrus sign and pregnancy. Data were analyzed descriptively. This study showed that OG and SOC with abnormal reproductive organs was 1 heifer (5.56%) and 7 heifers (36.84%), respectively. The responses of estrus synchronization in heifers with normal and abnormal reproductive organs were 94.17% and 100% in OG, and 100% and 42.86% in SOC. Meanwhile, the pregnancy rate was 70.59 and 0% in OG, and 50.00 and 0% in SOC. In conclusion, the number of SOC heifers with abnormal reproductive organs is higher than OG. Estrus synchronization using 50 mg medroxy progesterone acetate increase the amount of estrus and pregnancy in heifers with normal reproductive organs.

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RELATIONSHIP OF ELECTRICAL RESISTANCE OF VAGINAL MUCUS DURING OESTRUS WITH POST-AI PREGNANCY IN COWS

Bangladesh Journal of Veterinary Medicine ◽

10.3329/bjvm.v15i2.35520 ◽

2018 ◽

Vol 15 (2) ◽

pp. 113-117 ◽

Cited By ~ 2

Author(s):

M. Ahmed ◽

M. K. Chowdhury ◽

M. M. Rahman ◽

J. Bhattacharjee ◽

M. M. U. Bhuiyan

Keyword(s):

Pregnancy Rate ◽

Electrical Resistance ◽

Genital Tract ◽

Conception Rate ◽

Heat Detector ◽

The Mean ◽

The Difference ◽

Rectal Palpation ◽

Relationship Of ◽

In Pregnancy

Accurate detection of oestrus is a common problem for farmers to do AI timely resulting in conception failure in cows. The objective of the present study was to know the electrical resistance of vaginal mucus (ERVM) during oestrus to determine the best time of AI for improvement of conception in cattle. A total of 28 cows were inseminated with frozen thawed semen after detection of ERVM with Draminski estrus detector at Kendua Upazila Livestock Office under Netrakona district of Bangladesh. Pregnancy was diagnosed by per rectal palpation of genital tract 8-10 weeks after insemination. The mean ERVM in pregnant cows was 208.5 ± 31.2 Ω (163.3 to 263.3 Ω) and the ERVM in non-pregnant cows was 214.4 ± 36.6 Ω (153.3 to 280.0 Ω). Moreover, the pregnancy rate was higher in cows (80.0 to 83.3%) when ERVM during oestrus varied from 150 to 220 Ω than that of 221 to 280 Ω counterpart (36.4%). The difference in pregnancy rates between groups was significant (P<0.05). The present study indicates that Dramniski electrical heat detector may be used for prediction of time for AI in cows of Bangladesh. Up to 220 Ω ERVM during oestrus is better for higher conception rate than that of more than 220 Ω counterparts. Further studies with high number of inseminated cows should be conducted before wide application of this electrical heat detector under field conditions in Bangladesh.

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Use of Carazolol at Pre-Synchronized Timed Artificial Insemination in Cows

Acta Veterinaria Brno ◽

10.2754/avb200877010059 ◽

2008 ◽

Vol 77 (1) ◽

pp. 59-64

Author(s):

Ş. M. Pancarci ◽

Y. Öztürkler ◽

Ö. Güngör ◽

C. Kaçar ◽

S. Yildiz ◽

...

Keyword(s):

Beneficial Effect ◽

Corpus Luteum ◽

Artificial Insemination ◽

Adrenergic Receptor ◽

Jugular Vein ◽

Receptor Blocker ◽

Transrectal Ultrasonography ◽

Physiologic Saline ◽

Timed Artificial Insemination ◽

Rectal Palpation

Efficacy of the β-adrenergic receptor blocker (carazolol) at Timed Artificial Insemination (TAI) was investigated. Cows (n = 73) were pre-synchronized with two PGF2α injections given 14 d apart to initiate the Ovsynch protocol at early and middle luteal stages 14 d later, and received injections of GnRH and PGF2α seven d apart followed by GnRH 48 h later, and TAI 16 - 18 h later. Corpus luteum (CL) was detected via rectal palpation at the beginning of the Ovsynch protocol. Carazolol (Treatment I; n = 41) or physiologic saline (Treatment II; n = 32) were administered via jugular vein five min before TAI. Uterine tone was determined prior to infusion and at TAI via rectal palpation. Pregnancies were diagnosed with transrectal ultrasonography 40 ± 7 d after TAI. Uterine tone was 2.8 (1.4 - 5.3) times higher (P < 0.01) in Treatment I than that in Treatment II at TAI. Increase in uterine tone was affected by treatment × CL × parity (P < 0.05), and 66.7%, 75%, 52.6%, 100% and 16.7%, 33.3%, 25%, 28.6% in primiparous cows with CL and without CL, and in multiparous cows with CL and without CL in Treatments I and II, respectively. Pregnancy rates did not differ between treatments I (34.2%) and II (40.6%). In conclusion, no beneficial effect of carazolol administration prior to TAI was found except for the increased uterine tone.

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Morphological sperm abnormalities of different breeds of AI bull and its impact on conception rate of cows in AI programme

Bangladesh Journal of Veterinary Medicine ◽

10.3329/bjvm.v2i2.2555 ◽

1970 ◽

Vol 2 (2) ◽

pp. 129-135 ◽

Cited By ~ 2

Author(s):

MJU Sarder

Keyword(s):

Artificial Insemination ◽

Morphological Characteristics ◽

Dairy Farm ◽

Conception Rate ◽

Genetic Groups ◽

Sperm Abnormalities ◽

Total Head ◽

Service Conception ◽

Positive Correlations ◽

Rectal Palpation

The main objective of this study was to evaluate the effect of genetic groups of bulls on morphological characteristics of spermatozoa in relation to conception rate of first inseminated cows. For this purpose, 1390 ejaculates of semen were collected from 71 bull of six genetic groups at Central Cattle Breeding Station and Dairy Farm (CCBSDF), Savar, Dhaka; Rajabarihat Dairy Cattle Improvement Farm (RDCIF), Rajshahi, and District Artificial Insemination Centre, Rajshahi, from January 1997 to March 2002. A total of 20936 cows were inseminated by 40 technicians of different AI Sub-centre or points under the District Artificial Insemination Centre, Rajshahi, and pregnancy was confirmed on rectal palpation at 90 to 120 days after insemination. The average values of total head abnormalities, free loose head, mid-piece, tail abnormalities, proximal and distal cytoplasmic droplets, total tail abnormalities and total sperm abnormalities were 5.45%, 2.44%, 1.19%, 6.19%, 0.74%, 0.57%, 11.16% and 16.38%, respectively. Genetic groups of bull had significant (p < 0.05) effect on all the morphological characteristics of spermatozoa. The lower incidence of total sperm abnormalities (13.45%) was found in the genetic groups of 100% SL and higher (19.28%) in 75% F x 25% L bulls. The genetic groups of bulls had significant effect on first service conception rate of cows. The conception rate was higher in cows inseminated with 100% Local than those inseminated with genetic groups of 75% F x 25% L (53.13% vs 45.88%; p < 0.05). Significantly strong positive correlations were found between total head abnormalities and total sperm abnormalities (r = 0.828; p < 0.01) as well as between total tail abnormalities and total sperm abnormalities (r = 0.892; p < 0.01). It appears from the results that minimum total sperm abnormalities are found for pure breed bulls than cross-bred bulls, and 100% Local, 100% Friesian and 50% SL x 50% F bulls had better conception rates on cows.Key words: Spermatozoa; abnormalities; bull; genetic group; AI; conception ratedoi: 10.3329/bjvm.v2i2.2555Bangl. J. Vet. Med. (2004). 2 (2): 129-135

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ESTRUS RESPONSE HOLSTEIN FRIESIAN COW PERANAKAN WHO INJECTED PROSTAGLANDIN IN INTRAMUSCULAR AND INTRAUTERINE

Jurnal Peternakan Nusantara ◽

10.30997/jpnu.v3i2.929 ◽

2018 ◽

Vol 3 (2) ◽

pp. 81

Author(s):

Lilik Bawa Nuryanto ◽

Ristika Handarini ◽

Yanyan Setiawan

Keyword(s):

Pregnancy Rate ◽

Reproductive Function ◽

T Test ◽

Conception Rate ◽

Estrus Synchronization ◽

Estrous Cycles ◽

Holstein Friesian ◽

Reproductive Disease ◽

Estrus Response ◽

Rectal Palpation

This study was aimed to compare the different responses of cattle estrus and pregnancy rate FH (Friesian Holsein) were injected with the prostaglandin hormone by intramuscular and intra-uterine. This study was used 20 cows FH on condition of normal reproductive function, not in a state of pregnant (confirmed by rectal palpation), visually not fat and not skinny, not reproductive disease, normal estrous cycles. Cows were divided into two treatment, P1: estrus synchronization by intra muscular (IM), P2: estrus synchronization by intrauterine (IU). Data were analyzed by T test, to compare the differences between the two treatments with SPSS device. The results showed that the synchronization of pregnancy by using intrauterine more efisien. The conclusion of this research PGF2α hormone injections by intrauterinehas atendency response higher than by intra muscular injection method. Keyword : Non Return Rate, Service Per Conception, Conception Rate.

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Effect of barium selenate injections on fertility of Pirlak ewes subjected to estrus synchronization during non-breeding season

Medycyna Weterynaryjna ◽

10.21521/mw.5758 ◽

2017 ◽

Vol 73 (8) ◽

pp. 479-482 ◽

Cited By ~ 3

Author(s):

Mushap Kuru ◽

Osman Sogukpinar ◽

Mustafa Makav ◽

Nebi Cetin

Keyword(s):

Pregnancy Rate ◽

Breeding Season ◽

Litter Size ◽

Chorionic Gonadotropin ◽

Conception Rate ◽

Transrectal Ultrasonography ◽

Estrus Synchronization ◽

Equine Chorionic Gonadotropin ◽

Synchronization Protocol ◽

Lambing Rate

This study was aimed at determining the effect of barium selenate injections on the fertility of Pirlak ewes which had been subjected to progesterone-assisted estrus synchronization during the non-breeding season. A total of 150 Pirlak ewes between the ages of 2 and 5 years were used in the study. Sponges containing progesterone were inserted into the vaginas of the ewes for 11 (groups I and III) or 14 days (groups II and IV) for the purpose of estrus synchronization, and 500 IU equine chorionic gonadotropin (eCG) was administered on the day of sponge removal. In addition, barium selenate was injected s.c. immediately before estrus synchronization protocol in groups I and III. After the sponges had been removed, estrus was observed for four days. The ewes were mated with fertile rams. The pregnancy was determined by transrectal ultrasonography 30 days after mating. It was observed that the estrus rate, pregnancy rate, conception rate, lambing rate, twinning rate, and litter size were not statistically different (P > 0.05) between the groups, but estrus onset was different (P < 0.001). In conclusion, barium selenate injections did not affect the fertility parameters before progesterone-assisted synchronization was applied to the Pirlak ewes during the non-breeding season

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Post artificial insemination conception rate of a Brahman bull in selected areas of Bangladesh

10.33109/bjvmjj19fam4 ◽

2019 ◽

Vol 17 (1) ◽

Cited By ~ 1

Author(s):

M. J. S. Bhuiyan ◽

M. T. Islam ◽

N. S. Juyena ◽

M. M. U. Bhuiyan

Keyword(s):

Pregnancy Rate ◽

Artificial Insemination ◽

Conception Rate ◽

Beef Breed ◽

Holstein Friesian ◽

Frozen Semen ◽

Significant Difference ◽

The Difference ◽

The Government ◽

Animal Farm

Background: To mitigate the increasing protein demand in Bangladesh, recently the government has started a program to inspire the farmers for rearing Brahman as a beef breed. But, it is necessary to assess the performance of frozen semen of Brahman bull and the other factors that affect the rate of conception after Artificial Insemination (AI) before widespread rearing in Bangladesh. Methods: Data on AI performance, different factors and rate of conception were collected from 150 inseminated cows and heifers of Sadar upazila, Mymensingh. The cows were inseminated by trained technician of Research Animal Farm of Department of Surgery and Obstetrics, BAU, Mymensingh. Results: The overall conception rate was 55.3%. The conception rate in Holstein Friesian cross (68.9%) and Sahiwal cross (66.7%) cows were relatively higher than that of Local (46%) cows. Upon increasing age the service required for conception was irregular. The conception rate with respect to different parities ranged from 47.0% to 58.7%. Cows received insemination at parity 1-2 showed highest conception rate (60.0%) and cows received insemination at parity 3-7 showed lowest conception rate (47.0%). There was no significant difference between them on conception rate. The conception rate was observed higher (69.2%) in the cows which were inseminated in spring than cows were inseminated in summer (57.3%), winter (53.3%) and rainy (38.5%). The pregnancy rate in cows having BCS 2.5-3 was higher (60.7%) than that of 2.0 and 3.5-4.0. However, the difference in conception rate did not vary significantly among different BCS of cows (p>0.05). The present study indicated that the pregnancy rate in cows was higher (76.9%) that yield 6-10 L of milk than those of 0, 1-2 and 3-5 L counterpart. However, the variation in conception rate with different milk yield was significant (p<0.05). The pregnancy rate in cows that inseminated between 19-24 hours was higher (66.0%) than that of ≤12 and 13-18 hours. The variation in conception rate with different interval between estrus to AI was significant (P<0.05). Conclusion: Further studies using more population of cows and semen donors are needed to confirm this result as low number of cows and only one semen donor were used in this study.

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The effect of oxytocin on the time of ovulation, conception, and embryonic survival in swine

Canadian Journal of Animal Science ◽

10.4141/cjas96-081 ◽

1996 ◽

Vol 76 (4) ◽

pp. 541-545

Author(s):

G. W. Dyck

Keyword(s):

Key Words ◽

Artificial Insemination ◽

Subcutaneous Injection ◽

Conception Rate ◽

Transrectal Ultrasonography ◽

Embryo Survival ◽

Embryonic Survival ◽

Key Words Oxytocin

Three experiments were undertaken to assess the effect of a subcutaneous injection of oxytocin (OT, 10 IU) on the time of ovulation in 25 sows, conception rate and embryo survival in these sows, and conception rate and embryo survival in 82 crossbred gilts. The OT was given after the sows were detected in estrus (exp. 1) and after the first artificial insemination (AI) in exps. 2 and 3. The effect of a single AI or a second AI 18 h later on embryo survival in the gilts also was assessed. The gilts were from either a control (C) line or a line selected (S) for minimum backfat at market weight. In sows, OT versus no injection (NI) had no effect on the time of ovulation, as determined by transrectal ultrasonography (TU) when the injection was given at the detection of estrus (OT, 43.4 ± 3.4 h; NI, 47.3 ± 4.1 h), or after the first AI (OT 43.6 ± 3.2 h; NI 40.5 ± 3.0 h). Oxytocin had no effect on embryo survival in sows (OT, 78.1 ± 4.0%; NI, 76.4 ± 4.0%) or gilts (OT, 79.4 ± 3.0%; NI, 83.2 ± 2.2%). The number of inseminations had no effect on embryo survival (single, 80.3 ± 2.6%; twice, 82.0 ± 2.7%). Embryo survival was lower in S gilts (77.1 ± 2.8% vs. C, 87.1 ± 1.5%, P = 0.0092). These results show that OT had no effect on the time of ovulation in sows or embryo survival in sows or gilts. In gilts embryo survival was not altered by a second AI. Embryo survival was reduced in gilts selected for minimum backfat. Key words: Oxytocin, artificial insemination, ovulation, embryo survival

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Pregnancy rates and peripheral progesterone levels following Ovsynch or CIDR ovulation synchronization/timed artificial insemination protocols in postpartum dairy cows

Canadian Journal of Animal Science ◽

10.4141/cjas07150 ◽

2008 ◽

Vol 88 (3) ◽

pp. 457-461

Author(s):

M. Aali ◽

T. Pretheeban ◽

G. Giritharan ◽

R. Rajamahendran

Keyword(s):

Dairy Cows ◽

Key Words ◽

Pregnancy Rate ◽

Artificial Insemination ◽

Sampling Period ◽

Pregnancy Rates ◽

Key Words Pregnancy ◽

Milk Samples ◽

Timed Artificial Insemination ◽

Rectal Palpation

Peripheral progesterone (P4) concentrations as well as pregnancy rates (PR) between cows treated with Ovsynch or CIDR ovulation synchronization/TAI protocols were compared. Two hundred and twenty-seven postpartum lactating Holstein cows were randomly assigned to Ovsynch (n = 111) or CIDR (n = 116) ovulation synchronization/TAI protocols. Pregnancy was diagnosed by ultrasonography at day 35 after TAI and later confirmed by rectal palpation at day 60 post TAI. Milk samples were taken from the beginning of treatment (day -10) until day 35 after TAI (day 0) for P4 determination. Pregnancy rates for Ovsynch and CIDR groups of cows were 31 and 41%, respectively. The PR of ovulation synchronized cows for Ovsynch and CIDR groups were 49 and 69%, respectively. Progesterone levels during the sampling period were similar (P > 0.05) between Ovsynch and CIDR treated cows. Key words: Pregnancy rate, progesterone, Ovsynch, CIDR, cows

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233 Estrus Response and Pregnancy Rates of Beef Replacement Heifers Enrolled in Two Fixed-time Artificial Insemination Protocols, with or Without Pre-synchronization

Journal of Animal Science ◽

10.1093/jas/skab235.228 ◽

2021 ◽

Vol 99 (Supplement_3) ◽

pp. 125-126

Author(s):

Vitor R G Rodrigues Gomes Mercadante ◽

G Cliff C Lamb ◽

Nicky Oosthuizen ◽

Nicholas W Wege Dias ◽

Stefania Pancini ◽

...

Keyword(s):

Body Weight ◽

Pregnancy Rate ◽

Artificial Insemination ◽

Prolonged Exposure ◽

Prostaglandin F2α ◽

Fixed Time ◽

Transrectal Ultrasonography ◽

Beef Heifers ◽

Estrus Response ◽

Exogenous Progesterone

Abstract Pre-synchronization strategies with prolonged exposure to exogenous progesterone have the potential to alter estrus expression and improve fertility in replacement beef heifers. The objective of this study was to evaluate the estrus response and pregnancy rate of beef heifers enrolled in two fixed-time artificial insemination (TAI) protocols. A total of 306 heifers (14 ± 2 months of age; 372 ± 24 kg body weight [BW]) at three locations were enrolled in the study. Within location, heifers were stratified by age and BW and randomly assigned to one of two treatments: 1) PG-CIDR54 (n = 152), heifers were administered prostaglandin F2α (PGF; 25 mg) and a controlled internal drug releasing (CIDR) insert (Day -14), an injection of GnRH [100 μg (Day -7)], PGF injection and CIDR removal (Day 0), and a second injection of GnRH concurrently with TAI 54 ± 2 h later (Day 2); 2) 7-day CO-Synch+CIDR (CTRL; n = 154), heifers were administered GnRH (100 μg) and a CIDR insert (Day -7), PGF (25 mg) injection and CIDR removal (Day 0), and a second injection of GnRH concurrently with TAI 54 ± 2 h later (Day 2). All heifers received an estrus alert patch on Day 0 and were evaluated for patch activation at TAI on Day 2. Pregnancy was diagnosed by transrectal ultrasonography between 35 and 55 days after TAI. The percentage of heifers exhibiting estrus between Day 0 and 2 was greater (P < 0.01) in PG-CIDR54 compared with CTRL (77.0 and 39.6%, respectively). In addition, pregnancy rate to TAI tended (P = 0.08) to be greater for PG-CIDR54 compared with CTRL (48.0 and 38.3%, respectively). We conclude that beef heifers enrolled in the PG-CIDR54 protocol had increased estrus expression and tended to have greater pregnancy rate when compared with heifers enrolled in the 7-day CO-Synch+CIDR protocol.

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