Detection of Brucella abortus in Bovine Milk by Polymerase Chain Reaction
Keyword(s):
This study compares milk ring test and three different polymerase chain reaction techniques (direct DNA extraction by column purification system, alkaline DNA extraction, and filtrated milk), in order to identifyBrucella abortusinfection in bovine milk. Milk ring test sensitivity and specificity were 72% and 80%, respectively. While specificity of the three polymerase chain reaction techniques was 100%; sensitivity was 92%, 88% and 100%, respectively, for the three polymerase chain reaction procedures. We conclude that the filtered animal’s milk polymerase chain reaction is the best procedure to make the diagnosis ofB. abortusinfections.
1993 ◽
Vol 59
(12)
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pp. 4065-4073
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2014 ◽
Vol 2
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pp. 5-8
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1992 ◽
Vol 58
(9)
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pp. 2717-2722
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2018 ◽
Vol 13
(3)
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pp. 115
2016 ◽
Vol 40
(1)
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pp. 5-8
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2020 ◽
Vol 57
(3)
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pp. e166996
2008 ◽
Vol 20
(5)
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pp. 639-641
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