scholarly journals Comparison of Milk Ring Test; Serum Plate Agglutination Test and Polymerase Chain Reaction for the Detection of Bovine Brucellosis

2014 ◽  
Vol 2 (1) ◽  
pp. 5-8
Author(s):  
Shamim Saleha
Keyword(s):  
Polymerase Chain Reaction ◽  
Agglutination Test ◽  
Ring Test ◽  
Bovine Brucellosis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Test Serum ◽  
Milk Ring Test

scholarly journals Detection of Brucella abortus in Bovine Milk by Polymerase Chain Reaction

2010 ◽  
Vol 79 (2) ◽  
pp. 277-280 ◽  
Author(s):  
Ayman Al-Mariri ◽  
Nermeen Haj-Mahmoud
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Brucella Abortus ◽  
Bovine Milk ◽  
Ring Test ◽  
Chain Reaction ◽  
Purification System ◽  
Polymerase Chain ◽  
Direct Dna Extraction ◽  
Milk Ring Test

This study compares milk ring test and three different polymerase chain reaction techniques (direct DNA extraction by column purification system, alkaline DNA extraction, and filtrated milk), in order to identifyBrucella abortusinfection in bovine milk. Milk ring test sensitivity and specificity were 72% and 80%, respectively. While specificity of the three polymerase chain reaction techniques was 100%; sensitivity was 92%, 88% and 100%, respectively, for the three polymerase chain reaction procedures. We conclude that the filtered animal’s milk polymerase chain reaction is the best procedure to make the diagnosis ofB. abortusinfections.

scholarly journals DETEKSI BRUCELOSIS PADA SUSU SAPI DENGAN UJI POLYMERASE CHAIN REACTION (PCR)

2015 ◽  
Vol 9 (1) ◽  
Author(s):  
Susan M. Noor ◽  
Pratiwi Sudharmono ◽  
Asmarani Kusumawati ◽  
Anis Karuniawati
Keyword(s):  
Polymerase Chain Reaction ◽  
Ring Test ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Milk Ring Test

Penelitian ini bertujuan mendeteksi brucelosis pada sampel susu sapi dengan uji polymerase chain reaction (PCR) dan membandingkan tingkatsensitivitas dan spesifisitasnya dengan metode milk ring test (MRT). Sebanyak 24 sampel susu sapi yang dikoleksi secara aseptik dari lapang diuji PCR dan MRT. Hasil pengujian menunjukkan bahwa 79,17% (19/24) sampel susu positif brucelosis dengan uji PCR dan 83,33% (20/24) dengan uji MRT. Sensitivitas dan spesifisitas PCR mendeteksi brucelosis masing-masing sebesar 75 dan 100% dibandingkan dengan uji MRT.

Serum based polymerase chain reaction and enzyme linked immunosorbent assays for diagnosis of bovine brucellosis

2018 ◽  
Author(s):  
V. Naveen Kumar ◽  
M. Vijaya Bharathi ◽  
G. Selvaraju ◽  
K. Porteen ◽  
K. Vijayarani
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Specimen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Brucellosis ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Molecular Assays ◽  
Specific Pcr ◽  
Polymerase Chain ◽  
Sera Samples

Brucellosis is one of the economically important diseases in India and diagnosis of brucellosis using single test is cumbersome due to variation in sensitivity and specificity among the different test. The present study was aimed to assess the suitability of serum as clinical specimen in molecular diagnosis and evaluate the serology and molecular assays as in diagnosis of bovine brucellosis. A total of 821 bovine sera samples were subjected to indirect Enzyme Linked Immunosorbent Assay (i-ELISA) and serum based Polymerase Chain Reaction (PCR) assay. On serology 6.70 per cent positivity of brucellosis were reported and on PCR assay, 47 and 29 sera samples were positive for bcsp 31 genus specific and IS711 species specific PCR assay respectively with per cent positivity of 5.72 and 3.53. In comparison between serology and molecular test, 44 samples were positive for both assays and 11 and 3 samples were positive for serology and molecular assays individually. This study suggests that serum sample can be utilised as the choice of clinical specimen for both PCR assay and i-ELISA will be a future choice for the diagnosis of bovine brucellosis.

scholarly journals SEROLOGICAL AND MOLECULAR SURVEY OF Leptospira spp. AMONG CART HORSES FROM AN ENDEMIC AREA OF HUMAN LEPTOSPIROSIS IN CURITIBA, SOUTHERN BRAZIL

2014 ◽  
Vol 56 (6) ◽  
pp. 473-476 ◽  
Author(s):  
Mariane Angélica Finger ◽  
Ivan Roque de Barros Filho ◽  
Christian Leutenegger ◽  
Marko Estrada ◽  
Leila Sabrina Ullmann ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Endemic Area ◽  
Agglutination Test ◽  
Southern Brazil ◽  
Chain Reaction ◽  
Human Leptospirosis ◽  
Leptospira Spp ◽  
Polymerase Chain ◽  
Positive Correlations

Introduction: Cart horses are a re-emerging population employed to carry recyclable material in cities. Methods: Sixty-two horses were sampled in an endemic area of human leptospirosis. The microscopic agglutination test (MAT) and real-time polymerase chain reaction (qPCR) were performed. Results: A seropositivity of 75.8% with serovar Icterohaemorrhagiae in 80.8% of the horses was observed. Blood and urine were qPCR negative. MAT showed positive correlations with rainfall (p = 0.02) and flooding (p = 0.03). Conclusions: Although horses may be constantly exposed to Leptospira spp. in the environment mostly because of rainfall and flooding, no leptospiremia or leptospiruria were observed in this study.

An evaluation of melarsomine hydrochloride efficacy for parasitological cure in experimental infection of dairy cattle with Trypanosoma evansi in Thailand

Parasitology ◽  
2011 ◽  
Vol 138 (9) ◽  
pp. 1134-1142 ◽  
Author(s):  
MARC DESQUESNES ◽  
KETSARIN KAMYINGKIRD ◽  
TIMOTHÉE VERGNE ◽  
NACHAI SARATAPHAN ◽  
RODTIAN PRANEE ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Dairy Cattle ◽  
Experimental Infection ◽  
Intramuscular Injection ◽  
Trypanosoma Evansi ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chain Reaction ◽  
Polymerase Chain

SUMMARYMelarsomine hydrochloride can cure Trypanosoma evansi infection in camels at a dose of 0·25 mg/kg, but at that dose relapses occur in cattle. In our study, the efficacy of an intramuscular injection of melarsomine hydrochloride at 0·5 mg/kg was assessed in 3 normal and 3 splenectomized dairy cattle experimentally infected with a stock of T. evansi from Thailand. The animals were monitored for 5 months by haematocrit centrifugation, blood- or cerebrospinal fluid-mouse inoculation, polymerase chain reaction, the card agglutination test (CATT) for T. evansi, and the enzyme-linked immunosorbent assay‑T. evansi. Parasitological and DNA tests became and remained negative just after treatment. By the end of the experiment, CATT was negative and ELISA scores were below or very close to the cut-off value. One of the splenectomized cattle died from anaplasmosis during the experiment, but tested negative for surra. It was concluded that the parasites had been cleared from the cattle, and melarsomine hydrochloride at 0·5 mg/kg can be recommended for treatment against T. evansi infection in dairy cattle in Thailand. Further work is necessary to validate the efficacy of the treatment in the event of confirmed CSF-infection.

scholarly journals Diagnosing visceral leishmaniasis and HIV/AIDS co-infection: a case series study in Pernambuco, Brazil

2012 ◽  
Vol 54 (1) ◽  
pp. 43-47 ◽  
Author(s):  
Aracele Tenório de Almeida e Cavalcanti ◽  
Zulma Medeiros ◽  
Fábio Lopes ◽  
Luiz Dias de Andrade ◽  
Valéria de Melo Ferreira ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Bone Marrow Aspirate ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Direct Agglutination Test ◽  
Chain Reaction ◽  
Dipstick Test ◽  
Polymerase Chain

HIV/AIDS-associated visceral leishmaniasis may display the characteristics of an aggressive disease or without specific symptoms at all, thus making diagnosis difficult. The present study describes the results of diagnostic tests applied to a series of suspected VL cases in HIV-infected/AIDS patients admitted in referral hospitals in Pernambuco, Brazil. From a total of 14 eligible patients with cytopenias and/or fever of an unknown etiology, and indication of bone marrow aspirate, 10 patients were selected for inclusion in the study. Diagnosis was confirmed by the following examinations: Leishmania detection in bone marrow aspirate, direct agglutination test, indirect immunofluorescence, rK39 dipstick test, polymerase chain reaction and latex agglutination test. Five out of the ten patients were diagnosed with co-infection. A positive direct agglutination test was recorded for all five co-infected patients, the Leishmania detection and latex agglutination tests were positive in four patients, the rK39 dipstick test in three, the indirect immunofluorescence in two and a positive polymerase chain reaction was recorded for one patient. This series of cases was the first to be conducted in Brazil using this set of tests in order to detect co-infection. However, no consensus has thus far been reached regarding the most appropriate examination for the screening and monitoring of this group of patients.

scholarly journals Occurrence of F42 colonization factor in Escherichia coli strains isolated from piglets with diarrhea

2005 ◽  
Vol 25 (1) ◽  
pp. 31-33
Author(s):  
Mário Paulo A. Penatti ◽  
Alex S. Silva ◽  
Geórgio F. Valadares ◽  
Domingos S. Leite
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Strong Association ◽  
Agglutination Test ◽  
Chain Reaction ◽  
Gene Encoding ◽  
Newborn Piglets ◽  
Colonization Factor ◽  
Heat Stable ◽  
Polymerase Chain

The objective of this study was to determine the presence of the colonization factor F42 in 168 strains of Escherichia coli isolated from diarrheic stools of newborn piglets. The presence of F42 in 12 (7.1%) strains was detected with the agglutination test. Through the Polymerase Chain Reaction (PCR) of F42 positive strains, gene encoding enterotoxins (ST-I, ST-II, LT-I and LT-II) were detected. The finding of ST-I/ST-II genes in 50% of the strains, ST-I (16%) and ST-II (25%) indicates a strong association of FC F42 with heat-stable enterotoxins (91%). In contrast, the thermolabile enterotoxin (LT-I and LT-II) genes were not detected. Serogroups of F42 positive strains were determined, serogroup O8 being the most prevalent (41,7%). Other serogroups, as there are O9, O11, O18, O32, O35, O98 and O101, were also identified. Thus, FC F42 was confirmed as an additional factor of virulence in the pathogenesis of porcine colibacillosis.

scholarly journals Frequency of Leptospira spp. in sheep from Brazilian slaughterhouses and its association with epidemiological variables

2012 ◽  
Vol 32 (3) ◽  
pp. 194-198 ◽  
Author(s):  
Rodrigo Costa da Silva ◽  
Veruska Maia da Costa ◽  
Fabio Hiroto Shimabukuro ◽  
Virgínia Bodelão Richini-Pereira ◽  
Benedito Donizete Menozzi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bacterial Growth ◽  
Agglutination Test ◽  
The State ◽  
Rodent Control ◽  
Chain Reaction ◽  
Serum Samples ◽  
Leptospira Spp ◽  
Positive Serum ◽  
Polymerase Chain

Leptospirosis is a worldwide anthropozoonosis that infects livestock, including sheep as the carriers to other animals and humans. The present study aimed to determine the prevalence of Leptospira spp. in sheep from two slaughterhouses in the state of São Paulo, Brazil and its association with epidemiological variables. Serum samples from 182 sheep were evaluated for Leptospira spp. antibodies by microscopic agglutination test (MAT). Results indicated 34/182 (18.68%; CI95% 13.70-24.98%) positive serum samples, mainly to the serovar Copenhageni (17/34; 50%; CI95% 33.99-66.01%). Bacterial growth in the Fletcher medium was detected for 13/34 (38.24%; CI95% 23.87-55.08%) animals, and confirmed by Polymerase Chain Reaction (PCR) and sequencing for only two kidney samples from two animals. Thus, treatment and vaccination of sheep, besides rodent control, can be useful to prevent the infection in the studied region since sheep are important Leptospira spp. carriers, and its transmission to slaughterhouse workers is mainly through the manipulation of visceral tissues.

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