scholarly journals Changes of selected biochemical indicators and electrocardiography indicators in nutritional muscular dystrophy in calves

2012 ◽  
Vol 81 (2) ◽  
pp. 119-125 ◽  
Author(s):  
Katarzyna Żarczyńska ◽  
Przemysław Sobiech ◽  
Andrzej Pomianowski ◽  
Agata Waclawska-Matyjasik ◽  
Artur Stopyra ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Vitamin E ◽  
Lactate Dehydrogenase ◽  
Muscular Dystrophy ◽  
Glutathione Peroxidase ◽  
Wave Amplitude ◽  
Blood Samples ◽  
Biochemical Indicators ◽  
T Wave ◽  
Nutritional Muscular Dystrophy

The aim of this study was to estimate changes in selected biochemical indicators during nutritional muscular dystrophy of calves and to use electrocardiography technique in early diagnosis of this disease. The study was performed on 24 Holstein-Friesian calves of both sexes, divided into two groups. The first one was experimental group of 12 calves with symptoms of nutritional muscular dystrophy and the second one was control group of 12 clinically healthy calves administered a single i.m. injection of vitamin E + selenium preparation on the second day after birth. Blood samples were collected from all animals 3 × at 7-day intervals, starting on day 5. Blood serum was subjected to biochemical analyses to determine Se and vitamin E concentrations, aspartate aminotransferase, creatine kinase and lactate dehydrogenase activity levels. The activity of glutathione peroxidase was determined in whole blood samples. Electrocardiographic assessment was performed on day 19 in all calves. In the group of calves with symptoms of nutritional muscular dystrophy, serum Se and vitamin E concentrations were significantly (P ≤ 0.01) lower than in control, the same was true for the activity of glutathione peroxidase (P ≤ 0.01). The changes were accompanied by an increase (P ≤ 0.01) in the activity of aspartate aminotransferase, creatine kinase and lactate dehydrogenase. The electrocardiograms of experimental animals revealed elevated heart rate, accelerated sinus rhythm, increased P wave amplitude, shorter PR, QT and ST interval, narrower QRS complex, shorter T wave duration and insignificantly increased T wave amplitude; these results were indicative of arrhythmia which is observed at early stages of cardiomyopathy. This study is the first one in which electrocardiography technique was used to diagnose nutritional muscular dystrophy in calves.

scholarly journals Applicability of the protein-lipid profile and activity of lactate dehydrogenase isoenzymes for diagnosing nutritional muscular dystrophy in calves

2018 ◽  
Vol 62 (4) ◽  
pp. 503-509
Author(s):  
Katarzyna Żarczyńska ◽  
Przemysław Sobiech ◽  
Anna Snarska ◽  
Dawid Tobolski ◽  
Chandra Shekhar Pareek ◽  
...  
Keyword(s):  
Vitamin E ◽  
Lactate Dehydrogenase ◽  
Muscular Dystrophy ◽  
Serum Levels ◽  
The Body ◽  
Control Group ◽  
Extent Of Damage ◽  
Experimental Group ◽  
Nutritional Muscular Dystrophy ◽  
Lactate Dehydrogenase Isoenzymes

AbstractIntroduction: In calves, hyposelenosis degenerates skeletal muscles in different parts of the body. The extent of damage to muscle cells can be diagnosed by determining the activity of creatine kinase (CK), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH). The aim of this study was to analyse variations in the serum levels of LDH isoenzymes in calves with nutritional muscular dystrophy (NMD), to determine the applicability of this parameter for diagnosing NMD, and to describe the influence of hyposelenosis on total protein (TP), triglyceride (TG), and cholesterol (CHOL) levels.Material and Methods: Two groups of calves (n = six animals per group) were used. After birth, control group calves (SC) were intramuscularly administered 10 ml of a preparation containing selenium (Se) and vitamin E, and experimental group animals (SE) that were not injected. Blood was collected after 5, 15, and 25 days, and the concentrations of Se, vitamin E, TP, TG, and CHOL and the activity of glutathione peroxidase (GSH-Px), CK, and LDH fractions were determined.Results: Hypocholesterolaemia and elevated TG levels were found in SE group calves whose LDH fractions revealed a significant increase in LDH4 and LDH5 activity and a decrease in LDH1 activity when electrophoretically separated.Conclusions: Nutritional muscular dystrophy is accompanied by hypocholesterolaemia and elevated TG levels caused by muscle lipolysis. LDH4 and LDH5 activity parameters assist early diagnosis of NMD in calves.

scholarly journals Creatine Kinase and Lactate Dehydrogenase Responses After Different Resistance and Aerobic Exercise Protocols

2017 ◽  
Vol 58 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Gustavo A. Callegari ◽  
Jefferson S. Novaes ◽  
Gabriel R. Neto ◽  
Ingrid Dias ◽  
Nuno D. Garrido ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Resistance Exercise ◽  
Aerobic Exercise ◽  
Aerobic Training ◽  
Body Height ◽  
Blood Samples ◽  
Set Protocol ◽  
Experimental Protocols ◽  
Elevate Plasma

AbstractThe aim of this study was to investigate the responses of creatine kinase (CK) and lactate dehydrogenase (LDH) after performing different resistance and aerobic exercise protocols. Twelve recreationally trained men (age, 23.2 ± 5.6 years; body mass, 84.3 ± 9.3 kg; body height, 178.9 ± 4.5 cm; and BMI, 26.3 ± 2.3 kg·m2) volunteered to participate in this study. All subjects were randomly assigned to four experimental protocols (crossover): (a) aerobic training at 60% of VO2max, (b) aerobic training at 80% of VO2max, (c) a resistance exercise (RE) session with a bi-set protocol, and (d) an RE session with a multiple sets protocol. Blood samples were collected before, immediately after and 24 hours following the experimental protocols. After 24 hours, there was a significant increase in CK for the 80% of VO2max protocol vs. the bi-set RE session (p = 0.016). Immediately after the protocols, we observed a significant increase in LDH among certain groups compared to others, as follows: multiple sets RE session vs. 60% of VO2max, bi-set RE session vs. 60% of VO2max, multiple sets RE session vs. 80% of VO2max, and bi-set RE session vs. 80% of VO2max (p = 0.008, p = 0.013; p = 0.002, p = 0.004, respectively). In conclusion, aerobic exercise performed at 80% of VO2max appears to elevate plasma CK levels more than bi-set RE sessions. However, the bi-set and multiple sets RE sessions appeared to trigger greater levels of blood LDH compared to aerobic protocols performed at 60% and 80% of VO2max.

Adhesion of Malassezia pachydermatis of different growth type to canine epithelial cells

2014 ◽  
Vol 17 (2) ◽  
pp. 365-366 ◽  
Author(s):  
M. Florek ◽  
J. Król ◽  
Z. Staroniewicz ◽  
B. Bażanów
Keyword(s):  
Vitamin E ◽  
Epithelial Cells ◽  
Glutathione Peroxidase ◽  
Dairy Cows ◽  
Clinical Mastitis ◽  
Control Group ◽  
Growth Type ◽  
Malassezia Pachydermatis ◽  
Blood Samples ◽  
Experimental Group

Abstract This study focuses on the effect of parenteral administration of Selenium (Se) and vitamin E on concentration of Se in plasma and the activity of glutathione peroxidase (GPx) in the blood of dairy cows during peripartal period and their effect on the reduction of clinical mastitis. From a 220 individuals Holstein herd in a two-four lactation-gestation cycle the control group (C), 1st (D1) and 2nd (D2) experimental group were selected. Every group consisted of 15 cows in the last phase of the pregnancy. All cows were fed with the diet containing 0.1 mg of Se per kg/DM. The blood samples from vena jugularis were collected approximately 21 days before calving (control sampling), 3 days, 12 days and 21 days after calving. On the day of control sampling and 12 days before calving in D1 group, cows were injected subcutaneously in the sprescapular region with preparation Selevit inj. a.u.v. at the doses of 48.4 mg/head of Se, and 550 IU/head of α-Tocoferol (α-Toc). In D2 group, cows were injected by the same preparation only on 21th day before calving with the same doses of Se and α-Toc. The increase in the concentration of Se in the plasma and activity GPx in blood in D1 group on the 3rd day and 12th day after calving were determined. Increase in plasmatic concentrations α-Toc on 3rd day after calving and reduction of occurrence of clinical mastitis (13.3%) as compared with control group were found

scholarly journals Chronic Inflammation and Neutrophil Activation as Possible Causes of Joint Diseases in Ballet Dancers

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Leandro da Silva Borges ◽  
José Ricardo Bortolon ◽  
Vinicius Coneglian Santos ◽  
Nivaldo Ribeiro de Moura ◽  
Alexandre Dermargos ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Cardiac Muscle ◽  
Chronic Inflammation ◽  
Neutrophil Activation ◽  
Complement Component ◽  
Neutrophil Apoptosis ◽  
Joint Diseases ◽  
Blood Samples ◽  
Complement Component 3

Herein, we investigated the effects of a ballet class on the kinetic profiles of creatine kinase (CK) and lactate dehydrogenase (LDH) activities, cytokines, complement component 3 (C3), and the concentrations of immunoglobulin (Ig), IgA and IgM, in ballerinas. We also verified neutrophil death and ROS release. Blood samples were taken from 13 dancers before, immediately after, and 18 hours after a ballet class. The ballet class increased the plasma activities of CK-total (2.0-fold) immediately after class, while the activities of CK-cardiac muscle (1.0-fold) and LDH (3.0-fold) were observed to increase 18 hours after the class. Levels of the TNF-α, IL-1β, IgG, and IgA were not affected under the study conditions. The exercise was found to induce neutrophil apoptosis (6.0-fold) 18 hours after the ballet class. Additionally, immediately after the ballet class, the neutrophils from the ballerinas were found to be less responsive to PMA stimulus.Conclusion. Ballet class was found to result in inflammation in dancers. The inflammation caused by the ballet class remained for 18 hours after the exercise. These findings are important in preventing the development of chronic lesions that are commonly observed in dancers, such as those with arthritis and synovitis.

scholarly journals NUTRITIONAL MUSCULAR DYSTROPHY IN THE GUINEA PIG AND RABBIT

1931 ◽  
Vol 54 (2) ◽  
pp. 145-165 ◽  
Author(s):  
Marianne Goettsch ◽  
Alwin M. Pappenheimer
Keyword(s):  
Vitamin E ◽  
Muscular Dystrophy ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Unknown Factor ◽  
Nutritional Muscular Dystrophy

A diet is described, which leads to a progressive, highly selective, and ultimately fatal dystrophy of the voluntary muscles. Guinea pigs and rabbits are susceptible, rats resistant. The diet used is complete in known requirements, except for vitamin E; the addition of this factor, however, does not prevent the development of the disease. The lesions are not due to inanition, infection, or scurvy, and must be referred to some still unknown factor.

Study of effects of selenium and Vitamin E on Liver physiology in Fayoumi

2017 ◽  
Author(s):  
Safeer Abbas ◽  
Razia Iqbal ◽  
Muhammad Zeeshan Butt ◽  
Sadaf Niaz ◽  
Naser M. AbdEl-Salam ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Hepatoprotective Activity ◽  
Considerable Decrease ◽  
Blood Samples ◽  
Antioxidative Properties ◽  
Liver Physiology

Vitamin E is familiar for its antioxidative properties for neutralizing oxide radicals and plays a role to keep cells safe from physiological damage and Selenium being an integral part of Glutathione peroxidase assists Vit.E and help chicks grow normal and healthy. In current study four sets of chicken were given doses of vit. E and Selenium (0, 1 ml /10L; 1 ml /7.5L; 1 ml /5L) in water. The Chicken were grouped in four sets as A,B,C and D. The experiment was continued sixty days and5ml blood samples were collected from all the groups A, B, C and D at interval of 15 days. In Fayoumi chicks a decrease was noted in the value of ALAT in all experimental sets excluding D on 3rd and 4th sampling. A significant increase (p>0.05) was observed in the amount of ASAT in all treated groups excluding group C which showed decreased (p less than 0.05) levels of ASAT on 2nd and 3rd sampling. The level of Alkaline Phosphatase reduced considerably in all experimental sets except group C where a slight increase (p>0.05) was observed on 2nd sampling. A considerable decrease was also observed in the amount of Bilirubin in all experimental sets except group C where an insignificant increase (p>0.05) was observed on 2nd sampling. The research suggested that vitamin E increases organism’s ability to cope infections besides hepatoprotective activity in desi layer bird Fayoumi.

scholarly journals Some Effects of Prolonged Vitamin E Deficiency in The Rat

1939 ◽  
Vol 39 (6) ◽  
pp. 643-652 ◽  
Author(s):  
Archer John Porter Martin ◽  
Thomas Moore
Keyword(s):  
Vitamin E ◽  
Muscular Dystrophy ◽  
Skeletal Muscles ◽  
Vitamin E Deficiency ◽  
Leg Muscles ◽  
Brown Discoloration ◽  
Recent Developments ◽  
Preliminary Communication ◽  
Convoluted Tubules ◽  
Nutritional Muscular Dystrophy

IN an early preliminary communication we (Martin & Moore, 1936) reported that in rats maintained for prolonged periods on diets deficient in vitamin E a brown discoloration of the uterus occurred. Degeneration of the convoluted tubules of the kidneys was also found. In a more recent paper we (Martin & Moore, 1938) described a less intense discoloration in the skeletal muscles, which was localized in distribution. The muscles of the hindlegs, which displayed the paresis described by Ringsted (1935), were always noticeably discoloured. In both the uterine and leg muscles discoloration was found to be associated with muscular degeneration. The similarity of the condition in the leg muscles to the nutritional muscular dystrophy first reported by Goettsch & Pappenheimer (1931) in guinea-pigs was noted. The purpose of this communication is to give a detailed account of this work, including its most recent developments (Moore, 1939).

scholarly journals Amino Acids Changes and Muscle Damage During the 400 km Ultra Trail Gobi Race

2018 ◽  
Author(s):  
Meng Li ◽  
Yao Wu ◽  
Guangyuan Huang ◽  
Song Chen ◽  
Shengting Li ◽  
...  
Keyword(s):  
Amino Acids ◽  
Creatine Kinase ◽  
Amino Acid ◽  
Lactate Dehydrogenase ◽  
Muscle Damage ◽  
Male Athletes ◽  
Progressive Decline ◽  
Blood Samples ◽  
Mean Values ◽  
Ultra Endurance

Current researches demonstrated that completing the Ultra Trail Gobi (UTG) could lead to severe muscle damage. Our study was designed to analysis the muscle damage and amino acid changes reacted to a 400 km ultra-endurance race in experienced runners. Peripheral blood samples from 16 male athletes (mean age 40.3 ± 7.0 years, mean finish time 121.2 ± 21.8 hours), taken 48 h before and immediately after completing the Ultra Trail Gobi Race (UTG), were analyzed for 39 amino acids, 15 steroid hormones and 4 muscle damage factors. In all participants, the 4 biomarkers for muscle damage, i.e., creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) increased significantly after the race, whose mean post-race values were 13.7-, 7.3-, 4.7- and 1.5-fold higher than the pre-race values, respectively. 5 amino acids, i.e., alanine, valine, proline, ornithine and citrulline showed significant decrease, whose mean values decreased by 40.4 ± 18.7%, 38.9 ± 9.3%, 48.1 ± 15.2%, 44.8 ± 15.1% and 23.4 ± 30.8% after the race, respectively. Our study revealed that progressive decline in amino acids contents may further contribute to the factors increasing the muscle damage during the UTG.

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