scholarly journals Expanding Usutu virus circulation in Italy: detection in the Lazio region, central Italy, 2017 to 2018

2019 ◽  
Vol 24 (3) ◽  
Author(s):  
Fabrizio Carletti ◽  
Francesca Colavita ◽  
Francesca Rovida ◽  
Elena Percivalle ◽  
Fausto Baldanti ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Nucleic Acid ◽  
Blood Donation ◽  
Central Italy ◽  
Nucleic Acid Amplification ◽  
National Surveillance ◽  
Lazio Region ◽  
Nucleic Acid Amplification Tests ◽  
Usutu Virus ◽  
Mosquito Pool

Blood donation screening for West Nile virus (WNV) was mandatory in the Lazio region in 2017 and 2018 (June-November) according to the national surveillance plan. In these years, all five donations reactive in WNV nucleic acid amplification tests harboured instead Usutu virus (USUV). Clade ‘Europe 2’ was identified in four blood donations and a 2018 mosquito pool. The cocirculation of WNV and USUV in Lazio warrants increased laboratory support and awareness of possible virus misidentification.

Hepatitis C virus transmission by a blood donation negative in nucleic acid amplification tests for viral RNA

The Lancet ◽  
2000 ◽  
Vol 355 (9197) ◽  
pp. 41-42 ◽  
Author(s):  
Christian G Schüttler ◽  
Gregor Caspari ◽  
Christian A Jursch ◽  
Wulf R Willems ◽  
Wolfram H Gerlich ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Nucleic Acid ◽  
Blood Donation ◽  
Virus Transmission ◽  
Viral Rna ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Tests ◽  
Hepatitis C Virus Transmission

scholarly journals Screening of Mosquitoes for West Nile Virus and Usutu Virus in Croatia, 2015–2020

2021 ◽  
Vol 6 (2) ◽  
pp. 45
Author(s):  
Ana Klobucar ◽  
Vladimir Savic ◽  
Marcela Curman Posavec ◽  
Suncica Petrinic ◽  
Urska Kuhar ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Culex Pipiens ◽  
Rt Pcr ◽  
West Nile ◽  
Usutu Virus ◽  
Culex Pipiens Complex ◽  
Mammalian Origin ◽  
Mosquito Pool ◽  
The City ◽  
Generation Sequencing

In the period from 2015 to 2020, an entomological survey for the presence of West Nile virus (WNV) and Usutu virus (USUV) in mosquitoes was performed in northwestern Croatia. A total of 20,363 mosquitoes were sampled in the City of Zagreb and Međimurje county, grouped in 899 pools and tested by real-time RT-PCR for WNV and USUV RNA. All pools were negative for WNV while one pool each from 2016 (Aedes albopictus), 2017 (Culex pipiens complex), 2018 (Cx. pipiens complex), and 2019 (Cx. pipiens complex), respectively, was positive for USUV. The 2018 and 2019 positive pools shared 99.31% nucleotide homology within the USUV NS5 gene and both clustered within USUV Europe 2 lineage. The next-generation sequencing of one mosquito pool (Cx. pipiens complex) collected in 2018 in Zagreb confirmed the presence of USUV and revealed several dsDNA and ssRNA viruses of insect, bacterial and mammalian origin.

scholarly journals Is it time to move to nucleic acid amplification tests screening for pharyngeal and rectal gonorrhoea in men who have sex with men to improve gonorrhoea control?

Sexual Health ◽  
2011 ◽  
Vol 8 (1) ◽  
pp. 9 ◽  
Author(s):  
Christopher K. Fairley ◽  
Marcus Y. Chen ◽  
Catriona S. Bradshaw ◽  
Sepehr N. Tabrizi
Keyword(s):  
Nucleic Acid ◽  
Antimicrobial Resistance ◽  
Sexual Health ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Tests ◽  
Health Clinics ◽  
Sex With Men ◽  
Sexual Health Clinics

The use of nucleic acid amplification tests (NAAT), as well as or in preference to culture for non-genital sites is now recommended both in Australia and overseas because of their greater sensitivity and improved specificity. A survey of 22 Australian sexual health clinics who each year test over 14 500 men who have sex with men (MSM) show that culture remains the predominate method for detecting gonorrhoea at pharyngeal (64%) and rectal (73%) sites. This editorial discusses the potential disadvantages of using culture over NAAT in relation to optimal gonorrhoea control among MSM and advocates that significantly improved control would be achieved by moving to NAAT with the proviso that culture samples are taken wherever possible on NAAT-positive samples and from clients with urethritis to ensure continued surveillance for antimicrobial resistance.

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