scholarly journals Construction of scFv-antibodies to the active center of the Sunn bug (Eurygaster integriceps Put) gluten-hydrolyzing protease GHP3

2020 ◽  
Author(s):  
S. A. Timofeev ◽  
A. A. Tsarev ◽  
V. S. Zhuravlev ◽  
A. V. Konarev ◽  
V. V. Dolgikh
Keyword(s):  
Active Center ◽  
Single Chain ◽  
Eurygaster Integriceps ◽  
Immune Library ◽  
Selection Of

This work describes the preparation of immune library and selection of recombinant single-chain (scFv) antibodies to the active center of the gluten-destroying proteinase GHP3 of the Eurygaster integriceps Put. bug.

Expression of TIMP-1 in Pichia pastoris. Selection of an anti-TIMP-1 specific single-chain Fv antibody from a large non-immune library

2003 ◽  
Vol 327 (1-2) ◽  
pp. 171-179 ◽  
Author(s):  
Gordana Wozniak ◽  
Eva Obermayr ◽  
Matjaz Jeras ◽  
Mio Knezevic ◽  
Florian Rüker
Keyword(s):  
Pichia Pastoris ◽  
Single Chain ◽  
Single Chain Fv ◽  
Fv Antibody ◽  
Immune Library ◽  
Single Chain Fv Antibody ◽  
Selection Of

Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus

2007 ◽  
Vol 41 (1) ◽  
pp. 157-167 ◽  
Author(s):  
V. V. Dubrovskaya ◽  
A. B. Ulitin ◽  
A. G. Laman ◽  
I. P. Gileva ◽  
N. I. Bormotov ◽  
...  
Keyword(s):  
Variola Virus ◽  
Single Chain ◽  
Single Chain Antibodies ◽  
Immune Library ◽  
Selection Of

scholarly journals Selection of antibody single-chain variable fragments with improved carbohydrate binding by phage display

1994 ◽  
Vol 269 (13) ◽  
pp. 9533-9538
Author(s):  
S.J. Deng ◽  
C.R. MacKenzie ◽  
J. Sadowska ◽  
J. Michniewicz ◽  
N.M. Young ◽  
...  
Keyword(s):  
Phage Display ◽  
Carbohydrate Binding ◽  
Single Chain ◽  
Single Chain Variable Fragments ◽  
Selection Of

An innovative method for the selection of inhibitors of the viral spike-glycoprotein of the SARS-CoV

2020 ◽  
pp. 2050043
Author(s):  
T. V. Koshlan ◽  
K. G. Kulikov
Keyword(s):  
Active Center ◽  
Protein Interactions ◽  
Software Package ◽  
Wild Type ◽  
Spike Glycoprotein ◽  
Innovative Method ◽  
Detailed Method ◽  
Step Algorithm ◽  
Natural Peptides ◽  
Selection Of

This paper has developed and described a detailed method for selecting inhibitors based on modified natural peptides for the SARS-CoV BJ01 spike-glycoprotein. The selection of inhibitors is carried out by increasing the affinity of the peptide to the active center of the protein. This paper also provides a step-by-step algorithm for analyzing the affinity of protein interactions and presents an analysis of energy interactions between the active center of a protein and the wild-type peptide interacting with it, taking into account modifications of the latter. A description of the software package that implements the presented algorithm is given on the website https://binomlabs.com/covid19 .

Notes on parasites of Pentatomidae and Scutelleridae (Hemiptera-Heteroptera) in Middle East countries, with observations on biological control

1962 ◽  
Vol 53 (2) ◽  
pp. 241-256 ◽  
Author(s):  
E. S. Brown
Keyword(s):  
Biological Control ◽  
Middle East ◽  
Host Selection ◽  
Biological Control Agent ◽  
Control Agent ◽  
Sunn Pest ◽  
Eurygaster Integriceps ◽  
Middle East Countries ◽  
Central Turkey ◽  
Selection Of

A number of observations were made on parasites of certain Pentatomoids (sunn pest) attacking wheat in Middle East countries, during the course of other work on these pests. The principal host species studied were Eurygaster integriceps Put., E. maura (L.) and Aelia rostrata Boh. The data concern both Dipterous (Tachinid) endoparasites of the adult bugs, and also Hymenopterous (Scelionid) egg-parasites; the observations were made principally in central Turkey, and in the Varamine district of Iran. A few observations on predators were made also.The data concerning Tachinids consist of host records for six species of parasites. In no case observed by the author did these parasites appear to be of great importance in reducing the numbers of sunn pest; the same is true of the few instances of predators recorded.Observations were made on six species of Scelionid egg-parasites (five species of Asolcus and one of Hadronotus), five of which were bred from eggs of four species of Pentatomoid hosts collected in the field. The species concerning which the most comprehensive data were obtained were Asolcus rufiventris (Mayr), A. semistriatus (Nees) and A. vassilievi (Mayr). Egg-parasites are well known to be more efficacious in reducing sunn-pest populations, and A. semistriatus has been extensively used in Iran as a biological control agent. The data obtained enabled some ideas which are of importance for the use of egg-parasites in biological control to be formed and discussed. These concern principally (a) the susceptibilities of different hosts to parasitisation; (b) host selection by parasites; (c) the selection of a species of parasite most suitable for biological control in a particular area.

Selection of a Single Chain Variable Fragment Antibody against Ivermectin from a Phage Displayed Library

2010 ◽  
Vol 58 (9) ◽  
pp. 5387-5391 ◽  
Author(s):  
Shuang Wen ◽  
Xiao Zhang ◽  
Yuan Liu ◽  
Qingqing Zhang ◽  
Xianjin Liu ◽  
...  
Keyword(s):  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Selection Of

scholarly journals Selection of single chain antibody fragments binding to the extracellular domain of 4-1BB receptor by phage display technology

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769592 ◽  
Author(s):  
Salman Bagheri ◽  
Mehdi Yousefi ◽  
Elmira Safaie Qamsari ◽  
Farhad Riazi-Rad ◽  
Mohsen Abolhassani ◽  
...  
Keyword(s):  
Phage Display ◽  
Immunosorbent Assay ◽  
Specific Binding ◽  
Interleukin 2 ◽  
Extracellular Domain ◽  
Surface Glycoprotein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Single Chain ◽  
Single Chain Variable Fragments ◽  
Selection Of

The 4-1BB is a surface glycoprotein that pertains to the tumor necrosis factor–receptor family. There is compelling evidence suggesting important roles for 4-1BB in the immune response, including cell activation and proliferation and also cytokine induction. Because of encouraging results of different agonistic monoclonal antibodies against 4-1BB in the treatment of cancer, infectious, and autoimmune diseases, 4-1BB has been suggested as an attractive target for immunotherapy. In this study, single chain variable fragment phage display libraries, Tomlinson I+J, were screened against specific synthetic oligopeptides (peptides I and II) designed from 4-1BB extracellular domain. Five rounds of panning led to selection of four 4-1BB specific single chain variable fragments (PI.12, PI.42, PII.16, and PII.29) which showed specific reaction to relevant peptides in phage enzyme-linked immunosorbent assay. The selected clones were successfully expressed in Escherichia coli Rosetta-gami 2, and their expression was confirmed by western blot analysis. Enzyme-linked immunosorbent assay experiments indicated that these antibodies were able to specifically recognize 4-1BB without any cross-reactivity with other antigens. Flow cytometry analysis demonstrated an acceptable specific binding of the single chain variable fragments to 4-1BB expressed on CCRF-CEM cells, while no binding was observed with an irrelevant antibody. Anti-4-1BB single chain variable fragments enhanced surface CD69 expression and interleukin-2 production in stimulated CCRF-CEM cells which confirmed the agonistic effect of the selected single chain variable fragments. The data from this study have provided a rationale for further experiments involving the biological functions of anti-4-1BB single chain variable fragments in future studies.

Enrichment of a single clone from a high diversity library of phage-displayed antibodies by panning withAnopheles gambiae(Diptera: Culicidae) midgut homogenate

2003 ◽  
Vol 93 (1) ◽  
pp. 31-37 ◽  
Author(s):  
G.F. Killeen ◽  
B.D. Foy ◽  
R.H. Frohn ◽  
D. Impoinvil ◽  
A. Williams ◽  
...  
Keyword(s):  
Variable Region ◽  
Insoluble Fraction ◽  
Selection Methods ◽  
Single Chain ◽  
Single Clone ◽  
Native Electrophoresis ◽  
Cognate Antigen ◽  
Blocking Antibodies ◽  
High Diversity ◽  
Selection Of

AbstractA high diversity library of recombinant human antibodies was selected on complex antigen mixtures from midguts of femaleAnopheles gambiaeGiles. The library of phage-displayed single chain variable region fragment constructs, derived from β-lymphocyte mRNA of naïve human donors, was repeatedly selected and reamplified on the insoluble fraction of midgut homogenates. Five rounds of panning yielded only one midgut-specific clone, which predominated the resulting antibody panel. InA. gambiae, the epitope was found throughout the tissues of females but was absent from the midgut of males. The cognate antigen proved to be detergent soluble but very sensitive to denaturation and could not be isolated or identified by Western blot of native electrophoresis gels or by immunoprecipitation. Nevertheless, immunohistology revealed that this sex-specific epitope is associated with the lumenal side of the midgut. Severe bottlenecking may limit the utility of phage display selection from naïve libraries for generating diverse panels of antibodies against complex mixtures of antigens from insect tissues. These results suggest that the selection of sufficiently diverse antibody panels, from which mosquitocidal or malaria transmission-blocking antibodies can be isolated, may require improved selection methods or specifically enriched pre-immunized libraries.

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