Construction of a combinatorial immune library of human single-chain antibodies to orthopoxviruses and selection of antibodies to recombinant prA30L of the variola virus

AbstractFiloviruses, which include ebolaviruses and marburgvirus, can cause outbreaks of highly lethal hemorrhagic fever. This disease causes significant morbidity and mortality in humans and non-human primates, with human fatality rates reaching 90% during some outbreaks. Currently, there are a lack of licensed vaccines or antivirals for these viruses. Since early symptoms of filovirus infection mimic more common diseases, there is a strong unmet public health and biodefense need for broad-spectrum filovirus rapid diagnostics. We have generated a panel of mouse single-chain Fv-antibodies (scFvs) to filovirus glycoproteins (GPs) using cell-free ribosome display and determined their cross-reactivity profiles to all known filovirus species. Two scFvs (4-2 and 22-1) were able to detect all known Ebolavirus and Marburgvirus species. This is the first report on ribosome display scFvs that can detect a broad set of filovirus GPs, which demonstrates their potential use in the development of a new generation of rapid diagnostic immunoassays.

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Selection of human anti-human immunodeficiency virus type 1 envelope single-chain antibodies from a peripheral blood cell-based phage repertoire.

Journal of General Virology ◽

10.1099/0022-1317-79-12-2883 ◽

1998 ◽

Vol 79 (12) ◽

pp. 2883-2894 ◽

Cited By ~ 5

Author(s):

J J de Haard ◽

G van der Groen ◽

P Boender ◽

M J Koolen ◽

J W Arends ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Blood Cell ◽

Human Immunodeficiency Virus Type ◽

Peripheral Blood ◽

Peripheral Blood Cell ◽

Single Chain ◽

Single Chain Antibodies ◽

Immunodeficiency Virus ◽

Selection Of

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Construction of scFv-antibodies to the active center of the Sunn bug (Eurygaster integriceps Put) gluten-hydrolyzing protease GHP3

Abstract book of the 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology" PLAMIC2020 ◽

10.28983/plamic2020.247 ◽

2020 ◽

Author(s):

S. A. Timofeev ◽

A. A. Tsarev ◽

V. S. Zhuravlev ◽

A. V. Konarev ◽

V. V. Dolgikh

Keyword(s):

Active Center ◽

Single Chain ◽

Eurygaster Integriceps ◽

Immune Library ◽

Selection Of

This work describes the preparation of immune library and selection of recombinant single-chain (scFv) antibodies to the active center of the gluten-destroying proteinase GHP3 of the Eurygaster integriceps Put. bug.

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Selection of Single-Chain Antibodies against the VP8* Subunit of Rotavirus VP4 Outer Capsid Protein and Their Expression in Lactobacillus casei

Applied and Environmental Microbiology ◽

10.1128/aem.70.11.6936-6939.2004 ◽

2004 ◽

Vol 70 (11) ◽

pp. 6936-6939 ◽

Cited By ~ 8

Author(s):

Vicente Monedero ◽

JesÃ¯Â¿Â½s RodrÃ¯Â¿Â½guez-DÃ¯Â¿Â½az ◽

Rosa Viana ◽

Javier Buesa ◽

Gaspar PÃ¯Â¿Â½rez-MartÃ¯Â¿Â½nez

Keyword(s):

Lactobacillus Casei ◽

Probiotic Bacteria ◽

Extracellular Expression ◽

Outer Capsid Protein ◽

Single Chain ◽

Single Chain Antibodies ◽

Outer Capsid ◽

Selection Of

ABSTRACT Single-chain antibodies (scFv) recognizing the VP8* fraction of rotavirus outer capsid and blocking rotavirus infection in vitro were isolated by phage display. Vectors for the extracellular expression in Lactobacillus casei of one of the scFv were constructed. L. casei was able to secrete active scFv to the growth medium, showing the potential of probiotic bacteria to be engineered to express molecules suitable for in vivo antirotavirus therapies.

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