scholarly journals THE EFFECTS OF PALMVITEE ON HUMAN NASAL SEPTAL CHONDROCYTES CULTURE EXPANSION AND CARTILAGE RECONSTRUCTION

2017 ◽  
Vol 22 (3) ◽  
pp. 211
Author(s):  
B.H.I. Ruszymah ◽  
B.S. Aminuddin ◽  
A. Gapor ◽  
H. Fuzina
Keyword(s):  
Gene Expression ◽  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Pluronic F127 ◽  
Cartilage Tissue ◽  
Mice Model ◽  
Engineering Technology ◽  
Cartilage Reconstruction ◽  
Monolayer Expansion ◽  
And Cartilage

Palmvitee may act as a beneficial supplement to cartilage tissue engineering which is currently still has certain limitation on chondrocyte expansion and dedifferentiation. Our objectives in thisstudy were to evaluate the effects of Palmvitee on the growth kinetic and phenotype gene expression of human nasal septal chondrocytesin monolayer expansion as well as cartilage reconstruction via tissue engineering technology. Human chondrocytes were cultured inmediums containing various Palmvitee concentrations. Among the different test groups, the medium containing Palmvitee at 3 μg/ml supported the highest chondrocyte growth rate. The gene expression in monolayer chondrocytes culture supplemented with 3 μg/ml Palmviteedemonstrated similar results as in control. Cultured chondrocytes from the medium added with 3 μg/ml Palmvitee were then mixed with Pluronic F127 for cartilage reconstruction in nude mice model. Palmvitee supplementation supported the engineered cartilage development as shown in the histological and gene expression analysis on engineered tissue after 8 weeks of implantation. Therefore, Palmvitee at 3 μg/ml is beneficial for the human nasal septal chondrocytes monolayer expansion and cartilage engineering.

Starch-polycaprolactone based scaffolds in bone and cartilage tissue engineering approaches

2008 ◽  
pp. 337-356
Author(s):  
M.E. GOMES ◽  
J.T. OLIVEIRA ◽  
M.T. RODRIGUES ◽  
M.I. SANTOS ◽  
K. TUZLAKOGLU ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
And Cartilage

scholarly journals Gold Nanomaterials and Bone/Cartilage Tissue Engineering: Biomedical Applications and Molecular Mechanisms

2021 ◽  
Vol 9 ◽  
Author(s):  
Yifeng Shi ◽  
Xuyao Han ◽  
Shuang Pan ◽  
Yuhao Wu ◽  
Yuhan Jiang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Biomedical Applications ◽  
Rational Design ◽  
Molecular Mechanisms ◽  
Materials Science ◽  
Rapid Development ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Gold Nanomaterials ◽  
And Cartilage

Recently, as our population increasingly ages with more pressure on bone and cartilage diseases, bone/cartilage tissue engineering (TE) have emerged as a potential alternative therapeutic technique accompanied by the rapid development of materials science and engineering. The key part to fulfill the goal of reconstructing impaired or damaged tissues lies in the rational design and synthesis of therapeutic agents in TE. Gold nanomaterials, especially gold nanoparticles (AuNPs), have shown the fascinating feasibility to treat a wide variety of diseases due to their excellent characteristics such as easy synthesis, controllable size, specific surface plasmon resonance and superior biocompatibility. Therefore, the comprehensive applications of gold nanomaterials in bone and cartilage TE have attracted enormous attention. This review will focus on the biomedical applications and molecular mechanism of gold nanomaterials in bone and cartilage TE. In addition, the types and cellular uptake process of gold nanomaterials are highlighted. Finally, the current challenges and future directions are indicated.

scholarly journals Chondrocytes Proliferation of Patients with Cartilage Lesions in Their Own Body for Use in Cartilage Tissue Engineering: Hypotheses on aNew Approach for the Proliferation of Autologous Chondrocytes

2019 ◽  
Vol 8 ◽  
pp. 1483
Author(s):  
Zahra Abpeikar ◽  
Mostafa Soleimannejad ◽  
Akram Alizadeh
Keyword(s):  
Gene Expression ◽  
Tissue Engineering ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Cartilage Tissue Engineering ◽  
The Body ◽  
Cartilage Tissue ◽  
Wound Healing Process

Osteoarthritis is one of the most common chronic diseases, which have involved 250 million people around the world. One of the challenges in the field of cartilage tissue engineering is to provide an adequate source of chondrocytes to prevent changes in gene expression profile as a result of multiple passages.We hypothesized that by creating a low invasive lesion by scalpel or shear laser in the outer ear cartilage and stimulation of wound healing process, hyperplasia occurs and will provide an appropriate number of autologous chondrocytes for extraction and use in articular cartilage tissue engineering. Also, due to the effect of platelet-rich plasma and biomechanical forces in stimulating and accelerating of the repair process, these two factors can be used to achieve more desirable results.We describe a new approach to proliferate chondrocytes in the body. To evaluate this idea, various techniques of gene expression at the level of RNA or protein and animal experiments for histological studies can be used. Also, flowcytometry technique can be used to determine the cell viability and counting them.The use of autologous cell sources with minimal changes in gene expression profile can be promising in tissue engineering products. [GMJ.2019;8:e1483]

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