scholarly journals USING OF EPIDIDYMAL SPERMS OF LOCAL IRAQI BUCKS FOR IN VITRO FERTILIZATION

2013 ◽  
Vol 12 (2) ◽  
pp. 126
Author(s):  
I.H.S. AL-TIMIMI
Keyword(s):  
In Vitro Fertilization ◽  
Breeding Season ◽  
Normal Saline ◽  
Cumulus Cells ◽  
Vitro Fertilization ◽  
Fertilization Rates ◽  
High Level ◽  
Female Genital

Sixty female genital organs of black local Iraqi goats were collected from the abattoir during the breeding season, and transported in a normal saline in a cool box within 30 minutes of slaughter to the Theriogenology lab. The oocytes were collected either by aspiration or slicing and graded on the basis of the nature of cumulus cells and homogeneity of cytoplasm. The medium used for maturation and for IVF were TCM-199, TALP and MEM. Caudal epididymal sperms were used for IVF. Results of the present study showed a high level of maturation and fertilization rates observed in TCM-199, followed by TALP and MEM medium. Thus it could be concluded that there is a possibility of getting a successful IVF in Iraqi goats by using epididymal sperms.

scholarly journals A Brief Incubation of Cumulus-Enclosed Mouse Eggs in a Calcium-Free Medium Containing a High Concentration of Calcium-Chelator Markedly Improves Preimplantation Development

2020 ◽  
Vol 17 (10) ◽  
pp. 3505
Author(s):  
Valeria Merico ◽  
Silvia Garagna ◽  
Maurizio Zuccotti
Keyword(s):  
In Vitro Fertilization ◽  
Mammalian Species ◽  
Developmental Rate ◽  
Cumulus Cells ◽  
Fertilization Rate ◽  
Preimplantation Development ◽  
High Concentration ◽  
Vitro Fertilization ◽  
Positive Effects

The presence of cumulus cells (CCs) surrounding ovulated eggs is beneficial to in vitro fertilization and preimplantation development outcomes in several mammalian species. In the mouse, this contribution has a negligible effect on the fertilization rate; however, it is not yet clear whether it has positive effects on preimplantation development. Here, we compared the rates of in vitro fertilization and preimplantation development of ovulated B6C3F1 CC-enclosed vs. CC-free eggs, the latter obtained either after a 5 min treatment in M2 medium containing hyaluronidase or after 5–25 min in M2 medium supplemented with 34.2 mM EDTA (M2-EDTA). We found that, although the maintenance of CCs around ovulated eggs does not increment their developmental rate to blastocyst, the quality of the latter is significantly enhanced. Most importantly, for the first time, we describe a further quantitative and qualitative improvement, on preimplantation development, when CC-enclosed eggs are isolated from the oviducts in M2-EDTA and left in this medium for a total of 5 min prior to sperm insemination. Altogether, our results establish an important advancement in mouse IVF procedures that would be now interesting to test on other mammalian species.

In vitro fertilization of goat oocytes during the non-breeding season

1999 ◽  
Vol 35 (1) ◽  
pp. 49-54 ◽  
Author(s):  
S Samaké ◽  
E.A Amoah ◽  
S Mobini ◽  
O Gazal ◽  
S Gelaye
Keyword(s):  
In Vitro Fertilization ◽  
Breeding Season ◽  
Vitro Fertilization

Unstimulated in vitro fertilization revisited

1996 ◽  
Vol 5 (2) ◽  
pp. 129-138 ◽  
Author(s):  
Bradford Alan Kolb ◽  
Richard J Paulson
Keyword(s):  
In Vitro Fertilization ◽  
Luteinizing Hormone ◽  
Controlled Ovarian Hyperstimulation ◽  
Human Menopausal Gonadotropin ◽  
Ovarian Hyperstimulation ◽  
Vitro Fertilization ◽  
Fertilization Rates ◽  
Oocyte Yield ◽  
Follicle Aspiration

The first attempts at in vitro fertilization (IVF) of human oocytes were performed during cycles utilizing human menopausal gonadotropin (hMG) and human chorionic gonadotropin (hCG). These early cycles resulted in a successful conception, which unfortunately ended as a tubal gestation. The birth of Louise Brown in 1978, the first successful IVF birth, was actually achieved following fertilization during a spontaneous cycle in which ovulation was triggered with endogenous luteinizing hormone (LH).However, due to the greater margin for error afforded by larger numbers of follicles, the practice of IVF rapidly evolved towards the use of controlled ovarian hyperstimulation (COH) to achieve higher pregnancy rates. It is easy to understand why this approach evolved. Oocyte harvesting was accomplished primarily by laparoscopy. Since oocyte yield per follicle was less than 100% and fertilization rates were limited, the relatively traumatic follicle aspiration process was more likely to result in embryo transfer if a greater number of follicles was present.

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