scholarly journals Enrichment of organic complex compounds of coconut water and mungbean extract in chrysanthemum (Chrysanthemum morfolium L.) tissue culture media

2020 ◽  
Vol 486 ◽  
pp. 012123
Author(s):  
A Mollah ◽  
Kaimuddin ◽  
E Hamdi ◽  
F Haring ◽  
F Ulfa ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Complex Compounds ◽  
Coconut Water ◽  
Organic Complex ◽  
Tissue Culture Media

scholarly journals Pengaruh Komposisi Media dalam Perbanyakan Protocorm Like Bodies, Pertumbuhan Planlet, dan Aklimatisasi Phalaenopsis amabilis

2015 ◽  
Vol 4 (3) ◽  
pp. 131 ◽  
Author(s):  
Erick Raynalta ◽  
Dewi Sukma
Keyword(s):  
Tissue Culture ◽  
Survival Rate ◽  
Culture Medium ◽  
Culture Media ◽  
Coconut Water ◽  
Basic Medium ◽  
Water Medium ◽  
Media Composition ◽  
Fresh Weight ◽  
Tissue Culture Media

<p>ABSTRACT</p><p>Phalaenopsis  amabilis  orchid  is one   of the most  popular  species  of orchids and  was inaugurated as one of the  national  flower ' Puspa  Pesona'. The purpose  of this study  was to determine the effects of   various  tissue culture   media composition on  Protocorm Like Bodies (PLBs)  multiplication,  growth  of plantlets,  and  acclimatization  of  Phalaenopsis  amabilis. Basic medium  used was  a half strengh of  Murashige and  Skoog (1/2MS)  and  Hyponex with additional  coconut  water,  benzyladeninepurine,  and  chitosan.  In  PLBs multiplication experiments ,   ½  MS  +  15%  coconut  water  medium produces  the  highest   percentage  of survival  rate.  ½  MS  +  15%  coconut water  medium  and  Hyponex  2  g  L-1+  15%  coconut water  +  2.5 ppm chitosan  medium  gave  the best  effect on  the fresh   weight  of  plantlets. The composition of  the culture medium   significantly affect   the fresh   weight and length of   root   on 8  WAP  (week after planting)   in the   acclimatization stage.</p><p>Key words: benzyladeninepurine, chitosan, coconut water, Hyponex,  tissue culture</p><p>ABSTRAK</p><p>Phalaenopsis amabilis  yang dikenal dengan  nama anggrek bulan merupakan salah satu jenisanggrek  yang  paling  populer  dan  dinobatkan  sebagai  salah satu  bunga  nasional  dengan  sebutan ‘Puspa Pesona’.  Tujuan penelitian ini untuk  mengetahui pengaruh berbagai komposisi media kultur jaringan dalam perbanyakan  Protocorm Like Bodies  (PLBs),  pertumbuhan  planlet, dan aklimatisasi Phalaenopsis amabilis. Media dasar yang digunakan adalah setengah konsentrasi media  Murashige dan  Skoog  (1/2MS)  dan  Hyponex dengan  tambahan  air  kelapa,  benziladeninpurin  (BAP),  dan kitosan. Pada percobaan perbanyakan PLBs, media ½ MS + 15% air kelapa menghasilkan persentase hidup tertinggi.  Media ½ MS + 15% air kelapa dan Hyponex  2 g L-1+ 15% AK + 2.5 ppm  kitosanmemberikan  respon  terbaik  terhadap  bobot planlet.  Komposisi  media  kultur  berpengaruh  nyata terhadap bobot segar dan panjang akar pada 8-MST (minggu setelah tanam) dalam tahap aklimatisasi.</p><p>Kata kunci: benzylaminopurin, kitosan, air kelapa, Hyponex, kultur jaringan</p>

scholarly journals PEMANFAATAN LIMBAH BUAH PISANG DAN AIR KELAPA SEBAGAI BAHAN MEDIA KULTUR JARINGAN ANGGREK BULAN (Phalaenopsis amabilis) TIPE 229

2016 ◽  
Vol 11 (3) ◽  
pp. 373
Author(s):  
Ira Djajanegara
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Organic Wastes ◽  
Coconut Water ◽  
Adventitious Shoot Formation ◽  
Tissue Culture Media ◽  
Optimum Height ◽  
Subculture Period

Coconut water and rotten banana fruits are commonly found in traditional markets as organic wastes. One way to overcome the problems caused by these organic wastes is to convert these unuseful matter into an important and economically useful matter by using them as components of tissue culture media. One important commodity that is usually propagated by tissue culture is Phalaenopsis orchid type 229 (Phalaenopsis amabilis). Therefore, it would be more benefit to substitute the expensive chemicals with organic wastes such as coconut water and banana puree.In this experiment, addition of coconut water and banana pure to the minimum media containing commercial fertilizer red Polyhyponex, sucrose and commercial agar did not show any inhibition of Phalaenopsis orchid plantlet growth. This probably caused by sufficient macro and micro nutrients provided by those organic matter and Polyhyponexfertilizer. Moreover, addition of 100 mL/L of coconut water and 100 mg/L banana puree gave the optimum leaf and adventitious shoot formation. On the other hand, addition of 150 mL/L coconut water gave the optimum height and root formation. In this case,growing Phalaenopsis orchid plantlet should be done in 2 subculture period. The first subculture is to obtain maximum amount of leaf and shhot formation while the second subculture is to obtain optimum height and root formation.Key words : Waste, coconut water, banana puree, tissue culture, Phalaenopsis orchi

Fractional Factorial Design for Optimization of the SELDI Protocol for Human Adipose Tissue Culture Media

2007 ◽  
Vol 23 (1) ◽  
pp. 217-224 ◽  
Author(s):  
E. Szalowska ◽  
S.A.F.T. vanHijum ◽  
H. Roelofsen ◽  
A. Hoek ◽  
R.J. Vonk ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Adipose Tissue ◽  
Factorial Design ◽  
Fractional Factorial Design ◽  
Culture Media ◽  
Human Adipose Tissue ◽  
Fractional Factorial ◽  
Tissue Culture Media

scholarly journals Reversible oxidant-induced increases in albumin transfer across cultured endothelium: alterations in cell shape and calcium homeostasis

Blood ◽  
1985 ◽  
Vol 65 (3) ◽  
pp. 605-614
Author(s):  
DM Shasby ◽  
SE Lind ◽  
SS Shasby ◽  
JC Goldsmith ◽  
GW Hunninghake
Keyword(s):  
Tissue Culture ◽  
Xanthine Oxidase ◽  
Calcium Homeostasis ◽  
Actin Filaments ◽  
Cell Shape ◽  
Culture Media ◽  
Extracellular Calcium ◽  
Lanthanum Chloride ◽  
Tissue Culture Media ◽  
Induced Changes

To determine whether reactive oxygen molecules could directly and reversibly increase the transfer of albumin across an endothelial barrier, we measured albumin transfer across monolayers of endothelium cultured on micropore filters before and after exposure to xanthine and xanthine oxidase. Xanthine and xanthine oxidase increased endothelial albumin transfer in a dose-dependent fashion. Parallel phase contrast and fluorescence microscopy demonstrated retraction of adjacent cells from one another and disruption of the actin filaments. The oxidant- induced increases in albumin transfer and changes in cell shape were reversed by removing xanthine oxidase and then incubating the monolayers for 3 1/2 hours in tissue culture media enriched with fetal bovine serum. However, incubation in tissue culture media without serum resulted in progressive injury and cell death. Hence, the brief exposure to oxidants initiated a progressive injury process that was reversed by incubation in serum. Because intracellular and extracellular calcium are important determinants of cell shape, and because some oxidized membrane lipids act as calcium ionophores, we asked whether oxidants altered endothelial calcium homeostasis. Xanthine-xanthine oxidase increased release of 45Ca++ from preloaded cells. The calcium antagonist lanthanum chloride prevented xanthine- xanthine oxidase increases in endothelial albumin transfer and prevented the changes in cell shape; chelation of extracellular calcium inhibited lysis of endothelium by xanthine-xanthine oxidase; and the calcium ionophore A23187 increased endothelial albumin transfer and mimicked the oxidant-induced changes in cell shape. Lanthanum chloride inhibited these effects of A23187. These data suggest that oxygen radicals can reversibly increase endothelial permeability to macromolecules, that this is associated with reversible changes in endothelial cell shape and actin filaments, and that the changes in cell shape are related to oxidant-induced changes in endothelial calcium homeostasis.

Retinoic acid increases the sensitivity of the rat embryo fibroblast transformation assay

1988 ◽  
Vol 8 (4) ◽  
pp. 1845-1848
Author(s):  
T D Halazonetis ◽  
C Daugherty ◽  
P Leder
Keyword(s):  
Tissue Culture ◽  
Retinoic Acid ◽  
Culture Media ◽  
Rat Embryo ◽  
Transformation Assay ◽  
Tissue Culture Media ◽  
Focus Assay ◽  
Fibroblast Transformation

The rat embryo fibroblast focus assay is used to evaluate the transforming potential of several oncogenes. The sensitivity of this assay increased fivefold when retinoic acid was added to tissue culture media. Retinoic acid probably acts by selectively inhibiting the proliferation of nontransformed cells.

scholarly journals Usefulness of serology for the evaluation of Trypanosoma cruzi transmission in endemic areas of Chagas' disease

1989 ◽  
Vol 22 (3) ◽  
pp. 119-124 ◽  
Author(s):  
Roberto Chuit ◽  
Elisabet Subias ◽  
Analia C. Pérez ◽  
Irene Paulone ◽  
Cristina Wisnivesky-Colli ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Rural Areas ◽  
Culture Media ◽  
Previous Treatment ◽  
Insecticide Treatment ◽  
Tissue Culture Media ◽  
Indirect Hemagglutination ◽  
History Of

Thirteen communities from 7 Argentinian provinces were selected for the evaluation of serology as an indicator of transmission of Chagas disease. Of the communities appraised, 6 did not have a history of previous treatment with insecticides and 7 had received sporadic or continuous insecticide treatment. The inhabitants of 20% of the houses of each locality were studied by serology. The samples were obtained byfinger pricking and 50 fil of blood were mixed with 150μl of 50% glycerine solution in tissue culture media to be assayed by Indirect Hemagglutination and Indirect Immunofluorescence tests. In untreated areas, the prevalence of infection in infants 0-4 years old was 17.5%, reaching to over 22% for the 5-9 year old group, and to 33.3% in 10-14 year old individuals. The prevalence in treated and surveyed areas was 2.6% in 0-4 year old children, 5.4% in 5-9 year old and 6,2% in 10-14 year old youngsters. The differences between both areas were statistically significant (p < 0.005). This study favors serology as a valid indicator for the evaluation of transmission of Chagas disease in rural areas.

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