scholarly journals Genogeography of norway spruce (Picea abies (L.) Karst.) according to the analysis of cytoplasmic DNA

2021 ◽  
Vol 65 (4) ◽  
pp. 439-447
Author(s):  
V. E. Padutov ◽  
D. I. Kagan ◽  
S. I. Ivanovskaya ◽  
O. Yu. Baranov ◽  
T. S. Markevich
Keyword(s):  
Mitochondrial Dna ◽  
Picea Abies ◽  
Norway Spruce ◽  
Continuous Distribution ◽  
Distribution Area ◽  
Spruce Forest ◽  
The South ◽  
Allelic Variants ◽  
Regional Features ◽  
Ssr Loci

Norway spruce (Picea abies (L.) Karst.) is one of the main forest-forming species in Belarus. It plays important economic, ecological and social roles. The spruce forest of the region is characterized by a complex history of the formation of its population genetic structure. The aim of this study was the genogeographic analysis of P. abies populations and the description of regional features of its gene pool in Belarus. Molecular genetic analysis of microsatellite (SSR) loci of chloroplast DNA and mt15-D02 locus of mitochondrial DNA of Norway spruce was carried out for samples from 57 naturally originated forest stands. We identified 19 allelic variants of the Pt63718, Pt26081, Pt71936 cpDNA loci and two allelic variants of mt15-D02 mtDNA. The geographical distribution of the alleles has been described and the regional features of the genogeographic differentiation of the spruce forests have been considered. The southern border of the continuous distribution area of P. abies and its island localities lie in the south of Belarus (Brest and Gomel regions). The frequency of occurrence of individual allelic variants of cpDNA SSR loci has the most pronounced deviations from the average values for the whole country in those regions. Analysis of cpDNA showed the presence of certain regional features of the genogeographic structure of the spruce forest in the “south-north” and “west-east” directions. According to mitochondrial DNA analysis higher concentration of P. abies trees of southern (Carpathian) origin is observed in the southwest of Belarus. On the contrary northern (Boreal) origin dominates in the rest of the country. The results of performed genogeographic analysis of Norway spruce populations can serve as a basis for improving the forest seed zoning of the tree species.

scholarly journals Features of Picea abies (L.) Karst. population structure formation on the territory of Belarus

2021 ◽  
pp. 78-91
Author(s):  
Vladimir E. Padutov
Keyword(s):  
Mitochondrial Dna ◽  
Spatial Distribution ◽  
Picea Abies ◽  
Chloroplast Dna ◽  
Dna Analysis ◽  
Nuclear Dna ◽  
Spruce Forest ◽  
Allelic Variants ◽  
Regional Features ◽  
Migration Flows

One of the main forest forming tree species in Belarus is the European spruce (Picea abies (L.) Karst.). The formation of European spruce forest population genetic structure took place under the influence of migration flows from different refugia during the postglacial period. For the genogeographic study of P. abies 25 isozyme genes (Aat-1, Aat-2, Adh, Gdh, Idh-1, Idh-2, Mdh-1, Mdh-2, Mdh-3, Skdh, 6-Pgd-1, 6-Pgd-2, 6-Pgd-3, Lap-1, Lap-2, Sdh, Gpi, Hk, Me, Dia-1, Dia-2, Dia-4, Pgm-1, Pgm-2, Fl-Est) of nuclear DNA (analysis was carried out in 10 populations), 3 microsatellite loci (Pt63718, Pt26081, Pt71936) of chloroplast DNA (57 populations were considered) and 1 microsatellite locus (mt15-D02) of mitochondrial DNA (56 populations were studied) were used. As a result, 82 allelic variants of isozyme genes, 19 allelic variants of chloroplast DNA loci and 2 allelic variants of mitochondrial DNA locus were found. The spatial distribution of the alleles was defined and the regional features of the genogeographic differentiation of the spruce forest were considered. The presence of two migration flows representatives (southern and northern) in Belarus was confirmed. It was shown that the highest concentration of P. abies trees with southern (Carpathian) origin is observed in the southwest of the country. Clinal variability was revealed for a number of markers in the directions from south to north and from west to east. In general the data obtained are consistent with the results of studies based on the analysis of the spatial distribution of the cone scales traits.

Characterization of a mosaic minisatellite locus in the mitochondrial DNA of Norway spruce [Picea abies (L.) Karst.]

2003 ◽  
Vol 107 (3) ◽  
pp. 574-580 ◽  
Author(s):  
D. Bastien ◽  
J. M. Favre ◽  
A. M. Collignon ◽  
C. Sperisen ◽  
S. Jeandroz
Keyword(s):  
Mitochondrial Dna ◽  
Picea Abies ◽  
Norway Spruce ◽  
Minisatellite Locus

Differentiation among Austrian populations of Norway spruce [Picea abies (L.) Karst.] assayed by mitochondrial DNA markers

2006 ◽  
Vol 3 (3) ◽  
pp. 199-206 ◽  
Author(s):  
Fatemeh Maghuly ◽  
Franziska Nittinger ◽  
Wilhelm Pinsker ◽  
Werner Praznik ◽  
Silvia Fluch
Keyword(s):  
Mitochondrial Dna ◽  
Picea Abies ◽  
Norway Spruce ◽  
Dna Markers

scholarly journals Eradication of endophytes from needles, their inoculation with Lophodermium piceae and persistence of this fungus in needles of Norway spruce

2021 ◽  
Vol 20 (8) ◽  
pp. 981-992
Author(s):  
Michael M. Müller ◽  
Leena Hamberg
Keyword(s):  
Heat Treatment ◽  
Picea Abies ◽  
Norway Spruce ◽  
Insect Herbivory ◽  
Host Tree ◽  
Distribution Area ◽  
Needle Age ◽  
New Approach ◽  
Spruce Needles

AbstractLophodermium piceae is the most common endophyte of Norway spruce (Picea abies) needles, and it probably occurs in the total distribution area of Norway spruce. Its significance to the host tree is still largely unknown mainly because no methodology is known for generating endophyte-free saplings nor for inoculation of intact needles with this fungus. We report here how a daily heat treatment at 40 °C for 8 h eradicates endophytes of Norway spruce needles within a few days without causing visible harm to the plants. We compared various methods for inoculation of endophyte-free saplings. In order to obtain effective inocula, we investigated what factors activate dormant L. piceae infections in the needle tissue resulting in ascomata formation. Best inoculation success was achieved by suspending naturally shed, highly infected needles above the saplings. Infection frequencies of up to 70% of needles were achieved with repeated inoculations in the course of 3 years. Once established in needles, individual infections by L. piceae can persist for at least 5 years. Individual infections remain small in young needles, but at a needle age of 6 years, they start to grow and spread resulting in presence all over the needle from base to tip at a needle age of 7 years, but still without causing visible symptoms on the needles. The presented methodology for generating both endophyte-free and endophyte-infected saplings of Norway spruce provides a new approach for testing the significance of infections by L. piceae to insect herbivory and diseases of Norway spruce needles.

scholarly journals Short Note: Development of Mitochondrial Markers for Population Genetics of Norway Spruce [Picea abies (L.) Karst.]

2008 ◽  
Vol 57 (1-6) ◽  
pp. 41-44 ◽  
Author(s):  
F. Maghuly ◽  
K. Burg ◽  
W. Pinsker ◽  
F. Nittinger ◽  
W. Praznik ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Population Genetics ◽  
Mitochondrial Dna ◽  
Picea Abies ◽  
Norway Spruce ◽  
Population Genetic ◽  
Maternal Inheritance ◽  
Short Note ◽  
Genetic Studies ◽  
Mitochondrial Markers

AbstractNorway spruce is an important commercial tree species in northern and central Europe. Pure mitochondrial DNA isolated from tissue culture materials grown in the dark were used to construct a partial mitochondrial library. 100 clones were randomly selected and 19 markers were isolated. Three of these markers proved to be polymorphic and two showed maternal inheritance in controlled crosses. These markers will be useful for population genetic studies in P. abies.

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