Investigation of the effect of diazinon on CatSper 1 gene expression, sperm motility and germinal epithelium thickness in adult male mice

Arsenic is carcinogenic and teratogenic. In addition, it is also a developmental neurotoxicant. Little is known however about the effect of arsenic exposure during brain development on social behavior. This study aimed to detect the effect of developmental arsenic exposure on social behavior and related gene expression in C3H adult male mice. Pregnant C3H mice were exposed to sodium arsenite (NaAsO2, 85 ppm in the drinking water) from gestational day (GD) 8 to 18. The F1 generation male pups from different mothers were taken and social behavior tasks were examined. Social behavioral-related gene expression in the prefrontal cortex was determined by the real-time RT-PCR method. The mice with developmental arsenic exposure showed poor sociability and poor social novelty preference. Glutamate receptor expression (NMDA and AMPA receptor subunits) showed no significant difference, but gene expressions of serotonin receptor 5B (5-HT 5B) and brain-derived neurotrophic factor (BDNF) were significantly decreased (p < 0.05) in the arsenic-exposed group compared to control group. The heme oxygenase-1 (HO-1) and cyclooxygenase-2 (COX-2) gene expressions were not significantly different. Our findings indicate that developmental arsenic exposure might affect social behavior by modulating serotonin receptors and reducing BDNF. Some oxidative stress markers and inflammatory markers were not affected.

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Global Gene Expression Associated with Hepatocarcinogenesis in Adult Male Mice Induced by in Utero Arsenic Exposure

Environmental Health Perspectives ◽

10.1289/ehp.8534 ◽

2006 ◽

Vol 114 (3) ◽

pp. 404-411 ◽

Cited By ~ 50

Author(s):

Jie Liu ◽

Yaxiong Xie ◽

Danica M.K. Ducharme ◽

Jun Shen ◽

Bhalchandra A. Diwan ◽

...

Keyword(s):

Gene Expression ◽

Adult Male ◽

Arsenic Exposure ◽

Global Gene Expression ◽

In Utero ◽

Male Mice

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Ketogenic Diet Decreases Alcohol Intake in Adult Male Mice

Nutrients ◽

10.3390/nu13072167 ◽

2021 ◽

Vol 13 (7) ◽

pp. 2167

Author(s):

María del Carmen Blanco-Gandía ◽

Francisco Ródenas-González ◽

María Pascual ◽

Marina Daiana Reguilón ◽

Consuelo Guerri ◽

...

Keyword(s):

Gene Expression ◽

Ketogenic Diet ◽

Adult Male ◽

Ketone Bodies ◽

Standard Diet ◽

Male Mice ◽

Self Administration ◽

Nutritional Interventions ◽

Glucose Levels ◽

Gene Expression Analyses

The classic ketogenic diet is a diet high in fat, low in carbohydrates, and well-adjusted proteins. The reduction in glucose levels induces changes in the body’s metabolism, since the main energy source happens to be ketone bodies. Recent studies have suggested that nutritional interventions may modulate drug addiction. The present work aimed to study the potential effects of a classic ketogenic diet in modulating alcohol consumption and its rewarding effects. Two groups of adult male mice were employed in this study, one exposed to a standard diet (SD, n = 15) and the other to a ketogenic diet (KD, n = 16). When a ketotic state was stable for 7 days, animals were exposed to the oral self-administration paradigm to evaluate the reinforcing and motivating effects of ethanol. Rt-PCR analyses were performed evaluating dopamine, adenosine, CB1, and Oprm gene expression. Our results showed that animals in a ketotic state displayed an overall decrease in ethanol consumption without changes in their motivation to drink. Gene expression analyses point to several alterations in the dopamine, adenosine, and cannabinoid systems. Our results suggest that nutritional interventions may be a useful complementary tool in treating alcohol-use disorders.

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172-OR: Perinatal Exposure to a Western Diet Alters Hypothalamic Gene Expression and Predisposes to Diabetes in Adult Male Mice

Diabetes ◽

10.2337/db21-172-or ◽

2021 ◽

Vol 70 (Supplement 1) ◽

pp. 172-OR

Author(s):

MONA ELGAZZAZ ◽

ERIC D. LAZARTIGUES ◽

FRANCK MAUVAIS-JARVIS

Keyword(s):

Gene Expression ◽

Adult Male ◽

Western Diet ◽

Perinatal Exposure ◽

Male Mice

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Gene expression profiling in prepubertal and adult male mice using cDNA and oligonucleotide microarrays

10.33915/etd.1844 ◽

2003 ◽

Author(s):

Lisa Marie Tomascik-Cheeseman

Keyword(s):

Gene Expression ◽

Gene Expression Profiling ◽

Adult Male ◽

Expression Profiling ◽

Oligonucleotide Microarrays ◽

Male Mice

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Activation of Kiss1 neurons in the preoptic hypothalamus stimulates testosterone synthesis in adult male mice

10.1101/2020.12.03.410878 ◽

2020 ◽

Author(s):

Elisenda Sanz ◽

Jonathan C. Bean ◽

Richard D. Palmiter ◽

Albert Quintana ◽

G. Stanley McKnight

Keyword(s):

Gene Expression ◽

Adult Male ◽

Third Ventricle ◽

Serum Testosterone ◽

Mouse Genetics ◽

Specific Gene ◽

Male Mice ◽

Female Reproduction ◽

Steroid Synthesis ◽

Cell Type Specific

ABSTRACTKisspeptin-expressing neurons in the rostral periventricular region of the third ventricle (RP3V) play an essential role in female reproduction. However, adult male mice were reported to have very few Kisspeptin-expressing neurons in the RP3V compared to females. This led to the hypothesis that Kiss1RP3V neurons are responsible for the ability of females, but not males, to generate a surge of LH, triggering ovulation and steroid synthesis in the female. Using mouse genetics and cell type-specific gene expression analysis, we show that male mice harbor almost as many Kiss1RP3V neurons as the female and that gene expression in these neurons is very similar. Specific activation of male Kiss1RP3V neurons expressing viral-encoded hM3Dq caused a surge in serum testosterone levels. These results demonstrate that Kiss1RP3V neurons are present in the adult male and fully capable of regulating the hypothalamic/pituitary/gonadal axis. We suggest that these neurons may continue to play a role in reproductive behavior in adult male mice.

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