scholarly journals Changes in the Phospholipid Asymmetry of Buffalo Sperm Plasma Membrane in Relation to Cryopreservation Technology

2019 ◽  
Vol 39 (02) ◽  
pp. 289-292
Author(s):  
MG Ivanova
1991 ◽  
Vol 1061 (2) ◽  
pp. 185-196 ◽  
Author(s):  
Ajay P.S. Rana ◽  
Gopal C. Majumder ◽  
Suniti Misra ◽  
Amitabha Ghosh

1996 ◽  
Vol 270 (6) ◽  
pp. C1709-C1714 ◽  
Author(s):  
C. Foresta ◽  
M. Rossato ◽  
P. Chiozzi ◽  
F. Di Virgilio

We have identified the mechanism whereby extracellular ATP (ATPe) triggers the acrosome reaction in human spermatozoa. This nucleotide opens a ligand-gated ion channel expressed on the sperm plasma membrane. ATPe threshold and 50% effective concentration calculated on the total added ATPe are 0.1 and 2 mM, respectively, corresponding to a free ATP concentration (ATP4-) of 3 and 200 microM, respectively. The ATPe-gated channel is selective for monovalent cations (Na+, choline, and methylglucamine), whereas on the contrary, permeability to Ca2+ is negligible. Isosmolar replacement of extracellular Na+ with sucrose fully blocked ATPe-dependent sperm activation, thus suggesting a mandatory role for Na+ influx. These results show that human sperm express an ATPe-gated Na+ channel that might have an important role in sperm activation before egg fertilization.


2006 ◽  
Vol 120 (1) ◽  
pp. 33-44 ◽  
Author(s):  
P. C. N. Chiu ◽  
M.-K. Chung ◽  
R. Koistinen ◽  
H. Koistinen ◽  
M. Seppala ◽  
...  

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