Chemotherapeutic potiential of Lupeol and its analogue (oxime) on human mammary cancer cell line (MCF-7)

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Jayasri Gunasekaran ◽  
Mirunalini Sankaran
Keyword(s):  
Cell Line ◽  
Mammary Cancer ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Scavenging Activity ◽  
Mammary Cancer Cell ◽  
Underlying Mechanisms ◽  
Mcf 7 ◽  
Human Mammary Cancer

Lupeol, a dietary triterpene, present in many fruits and medicinal plants, has been reported to possess many pharmacological properties including anti-cancer activities both in vitro.The present study is conducted to investigate the anticancer activity and the underlying mechanisms of Lupeol analogue (oxime) on human mammary cancer (MCF-7).MTT assay showed that the lupeol analogue had marked cytotoxic activity in MCF-7 cancer cells.We observed increased levels of Antioxidants (ABTS, DPPH,Superoxide anions radical scavenging activity,Hydroxyl radical scavenging activity) in MCF-7 cell line, because of anti oxidant mechanism of lupeol analogue (oxime) .We observed the higher concentrations ( 20,30,40,50 and 70 µM/mL) of lupeol analogue  treatment significantly showed increased cytotoxicity in MCF-7 cells. The IC50 value was found to be 40 µM/mL of lupeol analogue (oxime) could greatly inhibit the cell growth. So, we have chosen at 40 and 320 µM/mL concentration of lupeol analogue (oxime) for further experiments. We observed increased levels of Lipid peroxidation and decreased levels of Antioxidants (SOD,CAT,GPx,GSH) in MCF-7 cell line  because of the pro oxidant mechanism of lupeol analogue (oxime) . These observations revealed the cytotoxic potential of lupeol, which could be attributed to their prooxidant property on the MCF-7 cells. It is evident from the observation made in the present study that the lupeol analogue (oxime)has potential anticancer effect compared to Lupeol.

scholarly journals Antioxidant and Anticancer Potential of Methanolic Leaf Extract of Moringa concanensis Nimmo Against Human Breast Cancer Cell Line MCF-7

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
S. Vijayakumar ◽  
V. Bhuvaneshwari ◽  
A. Sumathi
Keyword(s):  
Breast Cancer ◽  
Anticancer Activity ◽  
Cell Line ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Plant Sample ◽  
Crude Leaf Extract ◽  
Mcf 7

Breast cancer is one of the major health problems among women in both developed and developing countries. The objective of the present investigation is focused on the DPPH Radical Scavenging Activity and anticancer effect of the methanolic extract of Moringa concanensis Nimmo leaves against Breast cancer (MCF-7) cell line. The study was facilitated by collecting the plant sample and subjected to crude extraction. The anticancer activity of the crude methanolic leaf extract of M. concanensis against MCF- 7 cell line was examined by MTT assay. The present study confirms that the crude leaf extract of M. concanensis has potential anticancer activity against MCF- 7 cell lines while compared to the control. M. concanensis leaves possess remarkable anticancer property which may lead to development of novel compounds as natural phytomedicine.

Hyposmotically-Activated Efflux of L-Carnitine from a Human Mammary Cancer Cell Line

2001 ◽  
Vol 21 (6) ◽  
pp. 779-787
Author(s):  
D.B. Shennan
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Mammary Cancer ◽  
Osmotic Shock ◽  
Cancer Cell Line ◽  
Major Effect ◽  
Cell Swelling ◽  
Mammary Cancer Cell ◽  
Mammary Cancer Cell Line ◽  
Human Mammary Cancer

Cell-swelling, induced by a hyposmotic challenge, stimulated the efflux of L-carnitine from a human mammary cancer cell line, MDA-MB-231. The response was dependent upon the extent of the osmotic shock. Hyposmotically-activated L-carnitine efflux was inhibited by the anion transport blocker diiodosalicylate. The efflux of taurine from MDA-MB-231 cells was also stimulated by a hyposmotic shock via a pathway sensitive to diiodosalicylate. L-carnitine efflux from MDA-MB-231 cells was stimulated by isosmotic swelling in a manner which was inhibited by diiodosalicylate. The results suggest that L-carnitine may exit cells via a volume-sensitive pathway: it is possible that L-carnitine efflux may utilize the same pathway as amino acids. The efflux of L-carnitine via this route could have a major effect on the intracellular concentration of L-carnitine and could facilitate transepithelial L-carnitine transport.

scholarly journals ANTI-PROLIFERATIVE ACTIVITY OF ZIZYPHUS SPINA CHRISTI LEAVES METHANOL EXTRACT AGAINST RHABDOMYOSARCOMA (RD) CELL LINE

2017 ◽  
Vol 9 (2) ◽  
pp. 279 ◽  
Author(s):  
Ahmed R. Abu-raghif ◽  
Ghaith A. Jasim ◽  
Muneer M. Hanoon
Keyword(s):  
Antioxidant Activity ◽  
Cell Line ◽  
Proliferative Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Extraction Yield ◽  
Gas Chromatography Mass Spectrometry ◽  
Scavenging Activity

<p><strong>Objective: </strong>The aim of this study was to investigate the capability of <em>Zizyphus spina christi</em> methanol extract to inhibit cancer cell line proliferation.</p><p><strong>Methods: </strong>The leaves of <em>Zizyphus spina christi</em> were extracted by cold maceration method. The anti-proliferative activity of the methanol extract against rhabdomyosarcoma (RD) cell line was tested by 3-(4, 5 Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The phytochemical constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The antioxidant activity was assessed by measuring free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. <strong></strong></p><p><strong>Results: </strong>The percentage extraction yield for leaves with methanol was 20.64%. The methanol extract showed dose dependent inhibition of RD cell line, the IC<sub>50</sub> was 154.44 µg/ml. GC-MS showed the presence of flavonoid fraction and other compounds with antioxidant activity. The methanol extract demonstrated DPPH scavenging activity with IC<sub>50</sub> of 33.91 mg/ml.</p><p><strong>Conclusion: </strong>Methanol extract showed<strong> </strong>potential anti-proliferative activity against RD cell line,<strong> </strong>which could be due to its antioxidant activity.</p>

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