Chemotherapeutic potiential of Lupeol and its analogue (oxime) on human mammary cancer cell line (MCF-7)

Lupeol, a dietary triterpene, present in many fruits and medicinal plants, has been reported to possess many pharmacological properties including anti-cancer activities both in vitro.The present study is conducted to investigate the anticancer activity and the underlying mechanisms of Lupeol analogue (oxime) on human mammary cancer (MCF-7).MTT assay showed that the lupeol analogue had marked cytotoxic activity in MCF-7 cancer cells.We observed increased levels of Antioxidants (ABTS, DPPH,Superoxide anions radical scavenging activity,Hydroxyl radical scavenging activity) in MCF-7 cell line, because of anti oxidant mechanism of lupeol analogue (oxime) .We observed the higher concentrations ( 20,30,40,50 and 70 µM/mL) of lupeol analogue treatment significantly showed increased cytotoxicity in MCF-7 cells. The IC50 value was found to be 40 µM/mL of lupeol analogue (oxime) could greatly inhibit the cell growth. So, we have chosen at 40 and 320 µM/mL concentration of lupeol analogue (oxime) for further experiments. We observed increased levels of Lipid peroxidation and decreased levels of Antioxidants (SOD,CAT,GPx,GSH) in MCF-7 cell line because of the pro oxidant mechanism of lupeol analogue (oxime) . These observations revealed the cytotoxic potential of lupeol, which could be attributed to their prooxidant property on the MCF-7 cells. It is evident from the observation made in the present study that the lupeol analogue (oxime)has potential anticancer effect compared to Lupeol.