Effect of toxic gases on human erythrocytes: Spectrophotometric analysis.

Objective: This study was carried out to analyze the influence of various toxic gases on human erythrocytes. Study Design: Experimental study. Setting: Department of Chemistry, Hazara University, Mansehra. Period: January 2017 to June 2017. Material & Methods: Erythrocytes were isolated and prepared by the standard method from blood samples. The standard absorption spectra were recorded in the range of 200-800nm using double‐beam UV‐visible spectrophotometer. Gases were prepared in laboratory as per given protocol. Erythrocyte samples were treated with prepared gases. Absorbance of the samples was recorded. The spectrum of these treated samples was compared with the standard spectra of erythrocytes to check for any changes that might have occurred. Results: It was observed that erythrocytes exhibiteded altered behavior with each gas which was confirmed by shift in peaks in UV/Visible spectra. Absorption maxima were shifted from higher to lower wavelengths and this shift was more pronounced in case of Chlorine, Carbon Monoxide and Carbon Dioxide as compared to that observed in case of Hydrogen Sulfide, Sulfur dioxide, and Nitrogen Dioxide. Conclusion: This study showed that erythrocytes exhibit different behavior with different gases as evident by the shift of peaks in UV/Visible spectra. The absorption maxima were shifted towards lower wavelengths suggesting that these gases do exert an effect on human erythrocytes. The lipid site of erythrocytes, being sensitive for absorbing various atmospheric gases, caused deformation of original erythrocyte structure and altered their activity. This type of interaction of red blood cells with such external species, particularly the donor-acceptor case, caused damage to erythrocytes and in turn, decreased their number.

Download Full-text

UV SPECTROPHOTOMETRIC ANALYSIS AND VALIDATION OF OSELTAMIVIRPHOSPHATE IN PURE AND PHARMACEUTICAL FORMULATION

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2020v12i2.37505 ◽

2020 ◽

pp. 111-114 ◽

Cited By ~ 1

Author(s):

SACHIN A. YANJANE ◽

SHRISHAIL M. GHURGHURE ◽

VINOD K. MATOLE

Keyword(s):

Spectrophotometric Method ◽

Spectrophotometric Analysis ◽

Pharmaceutical Formulation ◽

Ich Guidelines ◽

Highly Sensitive ◽

Double Beam ◽

Recovery Study ◽

Uv Visible ◽

Oseltamivir Phosphate ◽

Simple Economic

Objective: A new, simple, economical, precise, sensitive, linear, accurate, rapid UV spectrophotometric method has been developed for the estimation of Oseltamivir Phosphate in pure form and pharmaceutical formulation. Methods: This UV method was developed using Methanol as a solvent. In the present method, the wavelength selected for analysis was 218 nm. UV-Visible double beam spectrophotometer (Systronic 2201) was used to carry out spectral analysis. The ICH guidelines were used to validate the method. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was found in the range of 10-50µg/ml. Accuracy was performed by using a recovery study. The amount of drug recovered was found to be in the range of 99.01-100.1%. The % RSD value was found to be less than 2. Conclusion: The developed UV spectrophotometric method was found to be simple, economic, sensitive, easy, accurate, linear, specific and highly sensitive and can be used for routine estimation of Oseltamivir Phosphate.

Download Full-text

UV Spectrophotometric Analysis and Validation of Dapsone in Semisolid Dosage Form

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00872 ◽

2021 ◽

pp. 5007-5009

Author(s):

Gajanand Nangare ◽

Varsha Tegeli ◽

Suyash Ingle ◽

Vinod Matole ◽

Avinash Birajdar ◽

...

Keyword(s):

Dosage Form ◽

Quantitative Estimation ◽

Spectroscopic Method ◽

Spectrophotometric Analysis ◽

Ich Guidelines ◽

Double Beam ◽

Recovery Study ◽

Uv Visible ◽

Routine Assay ◽

Stable Linear

Objectives: A new, economical, sensitive, simple, rapid UV spectrophotometric method has been developed for the estimation of Dapsone in pure form and Semisolid Dosage form. Method: This UV method was developed using methanol: RO water (75:25) as a solvent. In the present method the wavelength selected for analysis was 260nm. UV-Visible double beam spectrophotometer (Systronic 2201) was used to carry out spectral analysis. The ICH guidelines were used to validate the method. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was found in the range of 3-18µg/ml. Accuracy was performed by using recovery study. The amount of drug recovered was found to be in the range of 100.1-100.5 %. The %RSD value was found to be less than 2. Conclusion: The proposed UV spectroscopic method was found to be accurate, precise, stable, linear, specific, and simple for quantitative estimation of Dapsone in bulk and Semisolid dosage form. Hence the present UV spectroscopic method is suitable for routine assay of Dapsone in bulk and Semisolid dosage form.

Download Full-text

Infrared and UV-visible spectra of layer semiconductors Gas, GaSe and GaTe

Journal de Physique ◽

10.1051/jphys:0197700380100129300 ◽

1977 ◽

Vol 38 (10) ◽

pp. 1293-1299 ◽

Cited By ~ 18

Author(s):

U. Giorgianni ◽

G. Mondio ◽

P. Perillo ◽

G. Saitta ◽

G. Vermiglio

Keyword(s):

Visible Spectra ◽

Uv Visible

Download Full-text

Effect of NO2 substitution and solvent on UV-visible spectra, redox potentials and electron transfer mechanisms of copper β-nitrotriarylcorroles. Proposed electrogeneration of a Cu(I) oxidation state

Journal of Porphyrins and Phthalocyanines ◽

10.1142/s1088424616500899 ◽

2016 ◽

Vol 20 (07) ◽

pp. 753-765 ◽

Cited By ~ 2

Author(s):

Lina Ye ◽

Zhongping Ou ◽

Yuanyuan Fang ◽

Yang Song ◽

Bihong Li ◽

...

Keyword(s):

Absorption Spectra ◽

Soret Band ◽

Redox Potentials ◽

Nonaqueous Media ◽

Initial Assignment ◽

Visible Spectra ◽

Phenyl Rings ◽

Uv Visible ◽

Electron Reduction ◽

Transfer Mechanisms

Three copper triarylcorroles containing a [Formula: see text]-pyrrole nitro substituent were synthesized and characterized as to their spectral and electrochemical properties in nonaqueous media. The examined compounds are represented as [Formula: see text]-NO2(YPh)3CorCu, where Cor is the trianion of a triphenylcorrole and Y is a Cl, H or CH3 substituent at the para-position of the three meso-phenyl rings of the compound. The data from absorption spectra, electrochemistry and thin-layer spectroelectrochemistry are consistent with an initial assignment of Cu[Formula: see text]-Cor[Formula: see text] in CH2Cl2, DMF and pyridine and electrogeneration of a formal Cu(II) corrole with an unreduced macrocycle, represented as Cu[Formula: see text]-Cor[Formula: see text], after the first one-electron reduction in these solvents. The doubly reduced [Formula: see text]-nitrocorrole has a sharp Soret band at 439 nm and a well-defined Q-band at 611 nm in CH2Cl2. Similar absorption spectra are seen for the three examined doubly reduced nitrocorroles in DMF and pyridine, suggesting formation of a Cu(I) species with an unreduced macrocycle which is represented as Cu[Formula: see text]-Cor[Formula: see text]. Changes in redox potentials and absorption spectra of the nitrocorroles are examined as a function of solvent and substituents on the meso-phenyl rings of the compounds and comparisons are made between spectral and electrochemical data of the newly synthesized corroles and that of structurally related tetraarylcorroles lacking a [Formula: see text]-nitro group.

Download Full-text

Synthesis, Characterization, and Biological Studies of a Piperidinyl Appended Dipicolylamine Ligand and Its Rhenium Tricarbonyl Complex as Potential Therapeutic Agents for Human Breast Cancer

Bioinorganic Chemistry and Applications ◽

10.1155/2016/2675937 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 4

Author(s):

Amali Subasinghe ◽

Inoka C. Perera ◽

Svetlana Pakhomova ◽

Theshini Perera

Keyword(s):

Breast Cancer ◽

Anticancer Agents ◽

Human Breast Cancer ◽

Human Breast ◽

Tricarbonyl Complex ◽

Nmr Spectrum ◽

H Nmr ◽

Biological Studies ◽

Visible Spectra ◽

Uv Visible

A novel ligand bearing a central piperidinyl sulfonamide group, N(SO2pip)dpa, and its corresponding Re tricarbonyl complex, [Re(CO)3(N(SO2pip)dpa)]+, have been synthesized in good yield. The methylene CH2signal seen as a singlet (4.54 ppm) in a1H NMR spectrum of the ligand in DMSO-d6appears as two doublets (5.39, 5.01 ppm) in a spectrum of the [Re(CO)3(N(SO2pip)dpa)]+complex and confirms the presence of magnetically nonequivalent protons upon coordination to Re. Structural results revealed that the Re–N bond lengths fall within the normal range establishing coordination of ligand to metal. The presence of intraligandπ→π⁎andn→π⁎transitions is indicated by the absorption peaks around 200–250 nm in UV-visible spectra. Absorption peaks in UV-visible spectra around 300 nm for metal complexes were identified as MLCT transitions. The S–N stretch observed as a strong peak at 923 cm−1for N(SO2pip)dpa appeared at a shorter frequency, at 830 cm−1in an FTIR spectrum of the [Re(CO)3(N(SO2pip)dpa)]+. The intense fluorescence displayed by the N(SO2pip)dpa ligand has quenched upon coordination to Re. Relatively low IC50values given by human breast cancer cells, MCF-7, (N(SO2pip)dpa = 139 μM, [Re(CO)3(N(SO2pip)dpa)]+= 360 μM) indicate that N(SO2pip)dpa and [Re(CO)3(N(SO2pip)dpa)]+are promising novel compounds that can be further investigated on their usage as potential anticancer agents.

Download Full-text

UV-visible spectra and thermodynamic studies of 2′,5′-dideoxynucleosidylcobalamins

Journal of Inorganic Biochemistry ◽

10.1016/0162-0134(93)85027-6 ◽

1993 ◽

Vol 52 (2) ◽

pp. 109-120 ◽

Cited By ~ 3

Author(s):

Huilan Chen ◽

Hong Yan ◽

Wenxia Tang

Keyword(s):

Thermodynamic Studies ◽

Visible Spectra ◽

Uv Visible

Download Full-text

X-Ray, IR, NMR, UV-visible spectra and DFT analysis of 5-aryloxy-(1H)-tetrazoles, structure, conformation and tautomerism

Current Chemistry Letters ◽

10.5267/j.ccl.2014.1.001 ◽

2014 ◽

Vol 3 (2) ◽

pp. 85-96 ◽

Cited By ~ 1

Author(s):

Nader Noroozi Pesyan ◽

Sadeghali Bavafa ◽

Mohammad Samim Enayati ◽

Sajedin Hoseinpour ◽

Narges Ostadhosseini ◽

...

Keyword(s):

X Ray ◽

Visible Spectra ◽

Uv Visible

Download Full-text

DEVELOPMENT AND VALIDATION OF UV-SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF HYDROQUINONE IN BULK, MARKETED CREAM AND PREAPARED NLC FORMULATION

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2017v9i5.20467 ◽

2017 ◽

Vol 9 (5) ◽

pp. 102

Author(s):

Sukhjinder Kaur ◽

Taranjit Kaur ◽

Gurdeep Kaur ◽

Shivani Verma

Keyword(s):

Spectrophotometric Method ◽

Limit Of Detection ◽

Pharmaceutical Formulations ◽

Pure Form ◽

Nanostructured Lipid Carrier ◽

Limit Of Quantification ◽

Double Beam ◽

Uv Visible ◽

Development And Validation

Objective: The aim of the present work was to develop a simple, rapid, accurate and economical UV-visible spectrophotometric method for the determination of hydroquinone (HQ) in its pure form, marketed formulation as well as in the prepared nanostructured lipid carrier (NLC) systems and to validate the developed method.Methods: HQ was estimated at UV maxima of 289.6 nm in pH 5.5 phosphate buffer using UV-Visible double beam spectrophotometer. Following the guidelines of the International Conference on Harmonization (ICH), the method was validated for various analytical parameters like linearity, precision, and accuracy robustness, ruggedness, limit of detection, quantification limit, and formulation analysis.Results: The obtained results of the analysis were validated statistically. Recovery studies were performed to confirm the accuracy of the proposed method. In the developed method, linearity over the concentration range of 5-40 μg/ml of HQ was observed with the correlation coefficient of 0.998 and found in good agreement with Beer Lambert’s law. The precision (intra-day and inter-day) of the method was found within official RCD limits (RSD<2%).Conclusion: The sensitivity of the method was assessed by determining the limit of detection and limit of quantification. It could be concluded from the results obtained that the purposed method for estimation of HQ in pure form, in the marketed ointment and in the prepared NLC-formulation was simple, rapid, accurate, precise and economical. It can be used successfully in the quality control of pharmaceutical formulations and for the routine laboratory analysis.

Download Full-text