scholarly journals DNA Damage in Peripheral Blood Lymphocytes of Thyroid Cancer Patients After Radioiodine Therapy

2015 ◽  
Vol 57 (2) ◽  
pp. 173-179 ◽  
Author(s):  
U. Eberlein ◽  
H. Scherthan ◽  
C. Bluemel ◽  
M. Peper ◽  
C. Lapa ◽  
...  
Keyword(s):  
Dna Damage ◽  
Thyroid Cancer ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Radioiodine Therapy ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes

scholarly journals The prognostic value of DNA damage level in peripheral blood lymphocytes in germ cell cancer patients

2018 ◽  
Vol 29 ◽  
pp. viii325
Author(s):  
M. Mego ◽  
Z. Sestakova ◽  
K. Kalavska ◽  
L. Hurbanova ◽  
D. Jurkovicova ◽  
...  
Keyword(s):  
Dna Damage ◽  
Germ Cell ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Prognostic Value ◽  
Cell Cancer ◽  
Peripheral Blood Lymphocytes ◽  
Germ Cell Cancer ◽  
Damage Level ◽  
Blood Lymphocytes

Expression of critical genes used as prognostic biomarkers and DNA damage in lymphocytes from breast cancer patients

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 10641-10641 ◽  
Author(s):  
F. Franke ◽  
M. Agnoletto ◽  
J. Saffi ◽  
T. Guecheva
Keyword(s):  
Breast Cancer ◽  
Dna Damage ◽  
Dna Repair ◽  
Comet Assay ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Breast Cancer Patients ◽  
Dna Damage And Repair ◽  
Blood Lymphocytes

10641 Breast cancer is the most common malignancy among women and its rate of mortality is still high. The increase knowledge of breast cancer biology is heaving great impact on determining the clinical prognosis and response to treatment. Impaired DNA repair may elevate the risk of malignant transformation of breast cells due to the accumulation of spontaneous mutations in target genes and increasing susceptibility to exogenous carcinogens. The present study was designed to evaluate the relationship between DNA damage and expression of some critical genes including TP53, c-ERBB2, ER (Estrogen Receptor) and PR (Progesterone Receptor) in breast cancer. Blood samples were obtained from female patients with diagnosed breast cancer before chemotherapy as well as from healthy individuals, and were processed in 24 hours. To evaluate the role of DNA repair in breast cancer we determined the level of DNA damage and the capacity to remove DNA damage induced by hydrogen peroxide in the peripheral blood lymphocytes. For this purpose the alkaline version of the comet assay, which provides a sensitive tool to investigate DNA damage and repair, was applied. The level of basal DNA damage was higher in breast cancer patients compared to the control group. Considerable inter-individual variations of DNA damage and repair in breast cancer patients were observed both before and after the treatment. The correlation between DNA damage in peripheral blood and expression of p53, c-erbB-2, PR and ER was analyzed. This preliminary study indicates that the DNA damage accumulation, observed in peripheral blood lymphocytes of breast cancer patients in early stages, could be attributed to impaired DNA repair. Our results suggest that DNA damage, as evaluated by the comet assay, seems to be useful molecular biomarker for monitoring ongoing exposures to DNA damaging agents. Such a research on the mutagen sensitivity and efficacy of DNA repair could impact on the development of new diagnostic and screening strategies. Work Supported by FAPERGS and GENOTOX (UFRGS). No significant financial relationships to disclose.

scholarly journals Correlation between Micronuclei Frequency in Peripheral Blood Lymphocytes and Retention of 131-I in Thyroid Cancer Patients

2013 ◽  
Vol 229 (2) ◽  
pp. 115-124 ◽  
Author(s):  
Olgica B. Vrndic ◽  
Olivera M. Milo^|^scaron;evic-Djordjevic ◽  
Ljiljana C. Mijatovic Teodorovic ◽  
Marija Z. Jeremic ◽  
Ivana M. Sto^|^scaron;ic ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes

scholarly journals Blood cells in thyroid cancer patients: a possible influence of apoptosis

Open Medicine ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. 87-92
Author(s):  
Olgica B. Vrndic ◽  
Predrag M. Djurdjevic ◽  
Danijela D. Jovanovic ◽  
Ljiljana C. Mijatovic Teodorovic ◽  
Irena R. Kostic ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Blood Cell ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Lymphocytes ◽  
Color Flow ◽  
Blood Lymphocytes ◽  
Cell Counts ◽  
Blood Cell Counts

AbstractThe side effects of radioactive iodine (131-I) treatment of differentiated thyroid cancer (DTC) patients include reduction of peripheral blood cell counts. The aim of this study was to analyze some potential changes in blood cell counts of DTC patients after 131-I therapy, especially CD3-positive, CD19-positive, and CD56-positive peripheral blood lymphocytes (PBL), as well as the possible role of apoptosis in selected lymphocyte populations. The study group included 24 thyroid cancer patients and 24 control subjects. Peripheral blood samples from patients and controls were analyzed using 5-color flow cytometry. Apoptotic cells were detected using an Annexin V-FITC/7-AAD kit. There was a statistically significant decrease of all blood cells after the 131-I therapy. The CD19+ B lymphocyte population was the most affected (5.82 ± 3.21% before therapy vs. 3.93 ± 2.60% after therapy, p = 0.008). This decrease was correlated with the degree of apoptosis of peripheral blood lymphocytes (Spearman’s r = 0.563, p =0.013). We concluded that 131-I therapy of DTC patients led to a decrease of all peripheral blood cells, especially CD19+ B lymphocytes. This directly correlated with apoptosis of PBLs, indicating that radiation damage to B cells leads to subsequent elimination by apoptosis.

Sign in / Sign up

Export Citation Format

Share Document