scholarly journals Attenuation of Dictyocaulus filaria larvae by visible Laser radiation : 2- In vivo study

2000 ◽  
Vol 24 (2) ◽  
pp. 24-34
Author(s):  
S. A. G AL-S amarrae ◽  
F. J. K. AL- Mishhadani

Attenuated 3rd stage larvae of Dictyocaulus filaria, by continuos emission of visible Helium- Neon laser of 1mW and a wave length of 632.8 nm for 2.5,5,10 and 15 minutes, were dosed twice orally to four groups of five kids each. The first dose contained 1000 larvae and after five weeks a second dose of 2000 larvae was given . A 5 th group of five kids was left as control.  Results showed that attenuation at 2.5 min. exposure was insufficient as indicated by the increase in respiratory rate, eosinophilia and shading of larvae in the feaces. The results indicated that attenuation of Dictyocaulus filaria larvae at (5, 10 , and 15) min. exposure to laser radiation were efficient and safe to be used as attenuated vaccine .

1996 ◽  
Vol 14 (4) ◽  
pp. 175-177 ◽  
Author(s):  
G. BARBERIS ◽  
S. GAMRON ◽  
G. ACEVEDO ◽  
I. CADILE ◽  
H. JURI ◽  
...  

Author(s):  
Hugo Juri ◽  
Jose Palma ◽  
Frank Frank ◽  
Ron Lapin ◽  
Jose Lillo ◽  
...  

2018 ◽  
Vol 11 (1) ◽  
pp. 46-50
Author(s):  
Nikolay A Sergeev ◽  
Maxim S Shestakov ◽  
Elisaveta D Fomina

The influence of three types of low-intensive laser radiation to healing of lower extremities venous trophic ulcers in 82 patients was estimated (class VI, CEAP classification): the continuous radiation helium-neon laser (subgroup I), the modulated infrared laser radiation (subgroup II) and the combined laser radiation (pulse infrared radiation and continuous radiation of visible range) (subgroup III). The therapeutic laser devices such as "AFDL-1" (wavelength – 0,63 microns), "Scalar-1/40" (wavelength – 0,89 microns) and "Ulan-BL-20" (lengths of waves: 0,44; 0,52; 0,57; 0,64 and 0,89 microns) were used for conservative treatment of trophic ulcers.  The planimetric methods such as evaluation of the ulcer defects area and speed of its epithelization in dynamics were used for objective comparison of different types of laser radiation efficiency. Due to this treatment in all patients the complete healing of trophic ulcers was achieved. In patients of subgroup I the average speed of epithelization was 0,26 ± 0,05 cm²/days, in patients of subgroup II – 0,17 ± 0,06 cm²/days, in patients of subgroup III – 0,33 ± 0,05 cm²/days. The advantages of laser radiation in the visible range in isolated (helium-neon laser) and combined options are revealed. The best results are received in case of combined laser radiation generated by portable devices which provide distribution of low-intensive laser radiation within all effected tissues. The combination of traditional medical methods with combined laser radiation is expedient in conservative treatment of lower extremities venous trophic ulcers.


1990 ◽  
Vol 110 (5) ◽  
pp. 1561-1564
Author(s):  
E. Ya. Gatkin ◽  
A. L. Vladimirtseva ◽  
E. K. Balandina ◽  
A. P. �ttinger

1974 ◽  
Vol 20 (3) ◽  
pp. 294-296
Author(s):  
E. Yu. Andreeva ◽  
D. K. Terekhin ◽  
S. A. Fridrikhov

2012 ◽  
Vol 28 (3) ◽  
pp. 693-700 ◽  
Author(s):  
Bin Shu ◽  
Guo-Xin Ni ◽  
Lian-Yang Zhang ◽  
Xiang-Ping Li ◽  
Wan-Ling Jiang ◽  
...  

1991 ◽  
Vol 261 (1) ◽  
pp. H15-H21 ◽  
Author(s):  
H. Nishimura ◽  
W. I. Rosenblum ◽  
G. H. Nelson ◽  
S. Boynton

In the presence of circulating Evans blue a helium-neon laser injures the endothelium of brain surface arterioles in situ. The injury is known to selectively eliminate endothelium-dependent responses. The present study documents in mice the fact that such endothelial sites become selectively attractive to passing platelets that have been activated as a result of more severe injury upstream. We then test the hypothesis that the “capture” of platelets at the downstream site is due to loss of “classical” endothelial-dependent relaxing factor of acetylcholine (EDRFACh) at that site. EDRFACh is known to inhibit platelet adhesion/aggregation and is synthesized from L-arginine. We show that agents that would either enhance or reduce the synthesis of local EDRFACh reduce or increase the incidence of capture as predicted by the hypothesis. Thus NG-monomethyl-L-arginine (L-NMMA) and arginase that would reduce synthesis of EDRFACh enhanced platelet capture. L-Arginine, which would enhance synthesis of EDRFACh, inhibited platelet capture. In addition, L-NMMA and arginase enhanced platelet aggregation over the more severely damaged site upstream from the site of capture. Ex vivo studies of platelets harvested from treated mice showed that the platelets themselves were unaffected by the treatments that, except for arginase given intravenously, all involved topical application of the tested drugs.


2021 ◽  
Vol 25 (1) ◽  
pp. 91-104
Author(s):  
AL- Mashhadani, F.J. K. ◽  
AL-Samarrae, S.A. G

A comparative trial between the effect of 3rd stage Dictyocaulus filaria larval vaccine attenuated by continuous emission of visible Helium – Neon Laser of ImW and wave length of ( 632.8 ) nm of (5) minutes exposure that of gamma attenuated larvae at 0.5 k. gray ( Co as radiation Source ) was designed. Each of the two attenuated larval vaccines was given to a group of ( 5 ) kids each . Double immunization doses at ( 35 ) days interval from each vaccine were given orally. The first dose contained ( 1000 ) larvae and the second one ( 2000 ) for each . A challenge dose of ( 100 ) non- irradiated larvae /kg body weight was given after ( 5 ) week to the vaccinated groups and a control non- vaccinated (5 kids ) 3rd group. All animals were slaughtered after 6 weeks post challenge.  Results showed that both vaccines revealed 80.7% and 78.1% protection respectively for worm burden as compared to control. Laser attenuated vaccine exhibited a statistically significant inhibition in the fecundity of female worms and larvae secretion in faeces in comparison with the gamma irradiated vaccine. 


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