scholarly journals Investigation of Ram Sperm Acrosome Integrity in relation with Seminal Plasma Homocysteine and Nesfatin-1 Levels

2021 ◽  
Author(s):  
Caner OZTURK ◽  
Şükrü GÜNGÖR ◽  
Muhammed İNANÇ ◽  
Neşe Hayat AKSOY
Keyword(s):  
Seminal Plasma ◽  
Plasma Homocysteine ◽  
Acrosome Integrity ◽  
Ram Sperm ◽  
Sperm Acrosome

scholarly journals Seminal Plasma Proteins Revert the Cold-Shock Damage on Ram Sperm Membrane1

2000 ◽  
Vol 63 (5) ◽  
pp. 1531-1537 ◽  
Author(s):  
Beatriz Barrios ◽  
Rosaura Pérez-Pé ◽  
Margarita Gallego ◽  
Agustín Tato ◽  
Jesús Osada ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Plasma Proteins ◽  
Cold Shock ◽  
Seminal Plasma Proteins ◽  
Ram Sperm

Assessment of post-thawed ram sperm viability after incubation with seminal plasma

2012 ◽  
Vol 14 (2) ◽  
pp. 333-339 ◽  
Author(s):  
Mariana Rovegno ◽  
Weber Beringui Feitosa ◽  
Andre Monteiro Rocha ◽  
Camilla Mota Mendes ◽  
Jose Antonio Visintin ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Viability ◽  
Ram Sperm

scholarly journals Adición de proteínas del plasma seminal ovino durante la congelación del espermatozoide y efectos sobre su motilidad y viabilidad

2009 ◽  
Vol 10 (1) ◽  
pp. 51 ◽  
Author(s):  
Jaime Antonio Cardozo ◽  
Patricia Grasa ◽  
María Teresa Muiño B. ◽  
José Álvaro Cebrián P.
Keyword(s):  
Membrane Protein ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Gel Electrophoresis ◽  
Plasma Proteins ◽  
Two Dimensional ◽  
Plasma Séminal ◽  
Sperm Membrane ◽  
Seminal Plasma Proteins ◽  
Ram Sperm

<p>Este estudio se adelantó para evaluar el efecto de la adición de proteínas del plasma seminal de cordero en la criopreservación sobre la motilidad e integridad de la membrana espermática, y los cambios en el perfil electroforético de las proteínas de la membrana espermática inducidos por la criopreservación. Se usaron eyaculados de ocho corderos adultos de la raza rasa aragonesa, se les determinó su viabilidad y motilidad espermáticas y posteriormente se sometieron a un procedimiento de congelación. Las proteínas se separaron por el método de electroforesis en geles de acrilamida en dos dimensiones. Se obtuvo un mejoramiento significativo (<em>p </em>&lt; 0,05) en la calidad del semen congelado, cuando se adicionaron proteínas del plasma seminal. El análisis bidimensional comparativo entre el semen fresco y el congelado evidenció la pérdida de 8 puntos de proteína en el espermatozoide descongelado. La concentración de un punto de proteína de membrana espermática, de bajo peso molecular (punto 2), fue más alta (<em>p </em>&lt; 0,05) en el espermatozoide descongelado al que se adicionaron proteínas del plasma seminal. Se encontraron correlaciones entre algunos puntos de proteína y la motilidad y viabilidad espermáticas, lo cual sugiere que pueden jugar papeles importantes en el mantenimiento de la integridad y funcionalidad del espermatozoide. Se puede concluir que la adición de proteínas del plasma seminal en la congelación mejora la integridad del espermatozoide descongelado, y que la criopreservación del semen de cordero produce variaciones en la composición de las proteínas de membrana.  </p><p> </p><p><strong>Effect of seminal plasma proteins at freezing on ram sperm motility and viability</strong>  </p><p>The aim of the study was to evaluate the cryoprotective effect of seminal plasma proteins on ram sperm motility, membrane integrity and the changes in the profile of ram sperm membrane proteins induced by cryopreservation. Fresh ejaculates from 8 mature Rasa aragonesa rams were used. Sperm motility and cell viability was assessed. The freezing procedure was based on the method described by Fiser <em>et al</em>. (1987). Proteins extracted from fresh and frozen-thawed semen were subjected to the Two-dimensional polyacrilamide gel electrophoresis. A significant improvement in the quality of frozenthawed sperm was obtained after addition of seminal plasma proteins (<em>p </em>&lt; 0.05). Comparative two-dimensional polyacrilamide gel electrophoresis analysis between fresh and frozen semen, either with or without seminal plasma proteins in the cryopreservation medium, revealed that eight protein spots were lost in frozen-thawed sperm. The concentration of one sperm membrane protein spot of low Mr (spot 2) was higher (<em>p </em>&lt; 0.05) in proteinadded frozen sperm. Correlations found between certain protein spots sperm motility and viability suggests that these proteins could play important roles in the maintenance of sperm integrity and functionality. In conclusion, the addition of seminal plasma proteins to freezing extender improved frozen-thawed ram sperm integrity quality and cryopreservation of ram semen produced variations in the sperm membrane protein composition. </p>

Involvement of progesterone and estrogen receptors in the ram sperm acrosome reaction

2021 ◽  
Vol 74 ◽  
pp. 106527
Author(s):  
S. Gimeno-Martos ◽  
M. Santorromán-Nuez ◽  
J.A. Cebrián-Pérez ◽  
T. Muiño-Blanco ◽  
R. Pérez-Pé ◽  
...  
Keyword(s):  
Estrogen Receptors ◽  
Acrosome Reaction ◽  
Ram Sperm ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

scholarly journals Cholesterol-loaded cyclodextrin plus trehalose improves quality of frozen-thawed ram sperm

2019 ◽  
Vol 64 (No. 03) ◽  
pp. 118-124 ◽  
Author(s):  
ME Inanc ◽  
S Gungor ◽  
C Ozturk ◽  
F Korkmaz ◽  
I Bastan ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Mitochondrial Membrane ◽  
Improve Quality ◽  
Progressive Motility ◽  
Computer Aided ◽  
Acrosome Integrity ◽  
Ram Sperm ◽  
Additive Control ◽  
Artificial Vagina

The objective of this study was to determine effects of supplementing Tris-based semen extenders with either cholesterol-loaded cyclodextrin (CLC) or 7-dehydrocholesterol loaded cyclodextrin (7-DCLC) plus trehalose (T) for cryopreservation of ram semen. Semen was collected with an artificial vagina from five Merino rams (2–3 years of age) during the non-breeding season. Ejaculates were pooled, divided into eight equal portions, diluted with a standard Tris-based extender containing: no additive (control); T (50 mM); or T (50 mM) + 1.5, 2.5 or 3.5 mg of either 7-DCLC or CLC. Semen was chilled from 37°C to 4°C, placed in 0.25 ml French straws, held 5 cm above liquid nitrogen for 12 minutes, then plunged into liquid nitrogen. After thawing, a computer-aided semen analyzer system (CASA) was used to assess motility, whereas plasma membrane and acrosome integrity (PMAI) and high mitochondrial membrane potential (HMMP) were assessed with flow cytometry. Sperm supplemented with 2.5 mg and 3.5 mg CLC + T had the highest (P &lt; 0.05) total and progressive motility (65.2 ± 4.7 and 19.0 ± 1.0% respectively, mean ± SEM), albeit with no significant differences from sperm with 1.5 or 3.5 mg CLC + T. Sperm with 2.5 mg CLC + T had the highest (P &lt; 0.05) PMAI (59.3%; not different from 3.5 mg CLC + T) and highest (P &lt; 0.05) HMMP (64.6%; not different from 1.5 or 3.5 mg CLC + T). The lowest ALH value, 2.8 ± 0.3 µm was in the 2.5 mg 7-DCLC + T group; otherwise, there were no significant differences among groups for any other CASA end point. In conclusion, adding CLC + T to a tris-based extender optimized quality of frozen-thawed ram semen. Therefore, extenders including CLC + T have potential to improve quality of frozen-thawed ram sperm.

Boar seminal plasma inhibits cryo-capacitation of frozen-thawed ram sperm and improves fertility following intracervical insemination

2018 ◽  
Vol 105 ◽  
pp. 84-89 ◽  
Author(s):  
Yi Fang ◽  
Rongzhen Zhong ◽  
Xiaosheng Zhang ◽  
Jinlong Zhang ◽  
Daowei Zhou
Keyword(s):  
Seminal Plasma ◽  
Ram Sperm

Effect of zinc-binding proteins of seminal plasma on motility and acrosome integrity of canine spermatozoa stored at 5°C

2013 ◽  
Vol 13 ◽  
pp. 26-27
Author(s):  
M. Mogielnicka-Brzozowska ◽  
L. Fraser ◽  
A. Dziekońska ◽  
K. Tołścik ◽  
R. Strzeżek ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Binding Proteins ◽  
Zinc Binding ◽  
Acrosome Integrity

scholarly journals Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days

2016 ◽  
Vol 13 (1) ◽  
pp. 36-41 ◽  
Author(s):  
J. Api ◽  
I. Stani ◽  
S. Vakanjac ◽  
I. Radovi ◽  
A. Milovanovi ◽  
...  
Keyword(s):  
Protein Content ◽  
Seminal Plasma ◽  
Acrosome Integrity

scholarly journals Positive effects of trehalose and cysteine on ram sperm parameters

2017 ◽  
Vol 62 (No. 5) ◽  
pp. 245-252 ◽  
Author(s):  
S. Gungor ◽  
C. Ozturk ◽  
AD Omur
Keyword(s):  
Sperm Motility ◽  
Sperm Parameters ◽  
Control Group ◽  
Mitochondrial Activity ◽  
Sperm Viability ◽  
Significant Protection ◽  
Positive Effects ◽  
Liquid Storage ◽  
Acrosome Integrity ◽  
Ram Sperm

The aim of this study was to determine the effects of trehalose and cysteine on sperm motility, viability, mitochondrial activity and acrosome integrity during liquid storage of Merino ram semen. Ejaculates were collected using artificial vaginas from five Merino rams, microscopically evaluated and pooled at 37 °C. The pooled semen samples were diluted in a Tris-based extender, including cysteine (2 mM and 4 mM), trehalose (10 mM and 25 mM) and no antioxidant (control). Diluted semen samples were kept in tubes and cooled from 37 to 5 °C in a cold cabinet, and maintained at 5 °C. Cooled samples were evaluated for sperm motility, viability, mitochondrial activity and acrosome integrity at 0, 24, 48, 72 and 96 h. Extender supplemented with trehalose (10 and 25 mM) and cysteine (2 and 4 mM) led to higher motility in comparison to the control at 24, 48, 72 and 96 h of liquid storage (P &lt; 0.05). Trehalose at the doses of 10 mM, 25 mM and 2 mM cysteine led to higher viability between 24–48–72 h and at 96 h of liquid storage (P &lt; 0.05). Further, 4 mM of cysteine improved sperm viability rates at 24 and 48 h of storage compared to the control group (P &lt; 0.05), and resulted in improved acrosome integrity rates compared to the control group at 72 and 96 h of storage (P &lt; 0.05). Extender supplemented with 10 and 25 mM trehalose at 24 and 72 h and 4 mM cysteine at 24 and 96 h of storage led to higher sperm mitochondrial activity rates when compared to the control group (P &lt; 0.05). In conclusion, the findings of this study show that trehalose and cysteine provided significant protection to ram sperm parameters during liquid storage.

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