NUMBER OF BLACK-PIGMENTED ANAEROBIC BACTERIA CONTAINED IN THE GINGIVAL CREVICULAR FLUID (GCF) OF PATIENTS SUFFERING FROM THE CHRONIC PERIODONTITIS AND AGGRESSIVE PERIODONTITIS

Recently discovered interleukin 29 (IL-29) has antiviral properties and its production is induced by herpes viruses. This study was aimed at analyzing the effect of non-surgical periodontal treatment on IL-29 levels in gingival crevicular fluid (GCF) of chronic and aggressive periodontitis patients. A total of 60 participants were divided into healthy group (group 1;n= 20), chronic periodontitis group (group 2;n= 20), and aggressive periodontitis group (group 3;n= 20). GCF samples collected from each subject at baseline and 6–8 weeks after scaling and root planing were quantified for IL-29 levels using ELISA. The mean IL-29 concentration in GCF was found to be highest in group 3 (92.37 pg/μl). The mean IL-29 level in group 1 and group 2 was 36.88 pg/μl and 69.35 pg/μl respectively. After scaling and root planing, the mean concentration of IL-29 in GCF was increased to 85.99 pg/μl in group 2 and to 114.64 pg/μl in group 3. Results of the present study indicate that antiviral IL-29 level was highest in GCF of aggressive periodontitis patients and least in subjects with healthy periodontium, while that of chronic periodontitis lying in between. After non-surgical periodontal therapy, IL-29 levels increased both in chronic and aggressive periodontitis patients and deserve further investigation as a potential therapeutic agent in treating periodontitis.

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IL-17 and IL-11 GCF Levels in Aggressive and Chronic Periodontitis Patients: Relation to PCR Bacterial Detection

Mediators of Inflammation ◽

10.1155/2012/174764 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 30

Author(s):

Olfat G. Shaker ◽

Noha A. Ghallab

Keyword(s):

Chronic Periodontitis ◽

Dental Plaque ◽

Potential Role ◽

Molecular Detection ◽

Gingival Crevicular Fluid ◽

Periodontal Diseases ◽

Aggressive Periodontitis ◽

Enzyme Linked Immunosorbent Assay ◽

Polymerase Chain ◽

Crevicular Fluid

Objectives. This study evaluated IL-17 and IL-11 in gingival crevicular fluid (GCF) of generalized chronic periodontitis (GCP) and generalized aggressive periodontitis (GAgP) patients in relation to periodontopathic bacteria.Subjects and Methods. GCF samples were collected from 65 subjects including 25 CP, 25 GAgP, and 15 controls (C) and analyzed for IL-17 and IL-11 by an enzyme-linked immunosorbent assay. Molecular detection of bacteria in the dental plaque was determined by polymerase chain reaction.Results. The total amount of IL-17 was significantly higher in GAgP group than in GCP and C groups(P<0.001). The IL-11 concentration was significantly higher in C and GCP groups than GAgP group(P<0.001). The IL-11/IL-17 ratio was significantly higher in the C group than in GCP and GAgP groups(P<0.05). Moreover, GAgP group showed lower ratios of IL-11/IL-17 when compared to GCP group. The high positivity ofP. gingivalisin the dental plaque was associated with significantly increased GCF levels of IL-17 in GCP and GAgP patients.Conclusions. The increased IL-17 level in GCF of GAgP suggests a potential role in the aetiopathogenesis. Meanwhile, the decreased ratio of IL-11/IL-17 might reflect an imbalance between the proinflammatory and anti-inflammatory cytokines in different periodontal diseases.

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ESTIMATION OF PENTRAXIN-3 AND TUMOR NECROSIS ALPHA LEVELS IN GINGIVAL CREVICULAR FLUID OF CHRONIC PERIODONTITIS PATIENTS

Egyptian Dental Journal ◽

10.21608/edj.2017.75005 ◽

2017 ◽

Vol 63 (1) ◽

pp. 555-563

Author(s):

Ebtehal Hamdy ◽

Ibtesam Afifi

Keyword(s):

Chronic Periodontitis ◽

Tumor Necrosis ◽

Gingival Crevicular Fluid ◽

Pentraxin 3 ◽

Crevicular Fluid

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Are Proteinase 3 and Cathepsin C Enzymes Related to Pathogenesis of Periodontitis?

BioMed Research International ◽

10.1155/2014/420830 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Oya Türkoğlu ◽

Elif Azarsız ◽

Gülnur Emingil ◽

Necil Kütükçüler ◽

Gül Atilla

Keyword(s):

Gingival Crevicular Fluid ◽

Periodontal Diseases ◽

Study Groups ◽

Aggressive Periodontitis ◽

Control Group ◽

Proteinase 3 ◽

Cathepsin C ◽

Probing Depth ◽

Inflammatory Processes ◽

Crevicular Fluid

Aim. Cathepsin C is the activator of the polymorphonuclear leukocyte-derived proteinase 3, which contributes to inflammatory processes. The aim of the present study was to investigate gingival crevicular fluid (GCF) proteinase 3 and cathepsin C levels in periodontal diseases.Design. Eighteen patients with chronic periodontitis (CP), 20 patients with generalized aggressive periodontitis (G-AgP), 20 patients with gingivitis, and 18 healthy subjects were included in the study. Periodontal parameters including probing depth, clinical attachment level, papilla bleeding index, and plaque index were assessed in all study subjects. GCF proteinase 3 and cathepsin C levels were analyzed by ELISA.Results. GCF proteinase 3 total amount was significantly higher in diseased groups compared to control group, after adjusting ageP<0.05. No differences were found in GCF cathepsin C levels among the study groupsP>0.05. Periodontal parameters of sampling sites were positively correlated with GCF proteinase 3 total amountsP<0.01but not with cathepsin C total amountsP>0.05.Conclusions. Elevated levels of GCF proteinase 3 in CP, G-AgP, and gingivitis might suggest that proteinase 3 plays a role during inflammatory periodontal events in host response. However, cathepsin C in GCF does not seem to have an effect on the pathogenesis of periodontal diseases.

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