DNA-MARKER STUDY OF THE GENETIC POTENTIAL OF DOWNY MILDEW RESISTANCE IN HYBRID FORMS FROM VITIS AMURENSIS

DNA-marker analysis of grape genotypes for the presence of downy mildew resistance genes Rpv3 and Rpv12 was performed. Table and seedless grapes varieties and forms carring these resistance genes were identified according to the DNA-marker evaluation.

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Overexpression of a thaumatin-like protein gene from Vitis amurensis improves downy mildew resistance in Vitis vinifera grapevine

PROTOPLASMA ◽

10.1007/s00709-016-1047-y ◽

2016 ◽

Vol 254 (4) ◽

pp. 1579-1589 ◽

Cited By ~ 17

Author(s):

Rongrong He ◽

Jiao Wu ◽

Yali Zhang ◽

Cecilia B. Agüero ◽

Xinlong Li ◽

...

Keyword(s):

Vitis Vinifera ◽

Downy Mildew ◽

Protein Gene ◽

Vitis Amurensis ◽

Downy Mildew Resistance ◽

Mildew Resistance

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Identification of downy mildew resistance genes Rpv10 and Rpv3 by DNA-marker analysis in a Russian grapevine germplasm collection

Acta Horticulturae ◽

10.17660/actahortic.2019.1248.19 ◽

2019 ◽

pp. 129-134 ◽

Cited By ~ 1

Author(s):

Е. Ilnitskaya ◽

S. Tokmakov ◽

M. Makarkina ◽

I. Suprun

Keyword(s):

Downy Mildew ◽

Resistance Genes ◽

Dna Marker ◽

Germplasm Collection ◽

Downy Mildew Resistance ◽

Mildew Resistance ◽

Marker Analysis

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DNA marker identification of Rpv3 downy mildew resistance gene in seedless grape varieties

PLANT BIOTECHNOLOGY AND BREEDING ◽

10.30901/2658-6266-2019-3-o4 ◽

2020 ◽

Vol 2 (3) ◽

pp. 15-19

Author(s):

E. T. Ilnitskaya ◽

M. V. Makarkina ◽

S. V. Tokmakov

Keyword(s):

Downy Mildew ◽

Resistance Gene ◽

Resistance Genes ◽

North American ◽

Dna Marker ◽

North American Species ◽

Downy Mildew Resistance ◽

Mildew Resistance ◽

Seedless Grape ◽

Grape Varieties

Table grapes are a valuable dietary product. Seedless grapes are in high demand among consumers. For this reason, the breeding of seedless varieties is one of the popular trends in modern viticulture, along with the production of environmentally friendly products. Downy mildew (Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni) is one of the most common fungal diseases of the grapevine. Most downy mildew resistant grape accessions belong to North American species like Vitis aestivalis Michx., V. berlandieri Planch., V. cinerea (Engelm. ex A. Gray) Engelm. ex Millard, V. riparia Michx., V. rupestris Scheele, etc. The search for donors of resistance genes is an urgent task. Rpv3 is one of the most significant resistance genes from a number of North American grape varieties. The aim of this work is to identify the downy mildew resistance gene Rpv3 in seedless grape varieties by means of DNA-marker analysis. The grape varieties with rudimentary development of seed in berries and with North American species in the pedigree were chosen as the object of the study. The varieties “Dunavski lazur” and “Seyve Villard 12-375” with reference alleles were used as the positive control, while V. vinifera L. was used as the negative control. UDV305 and UDV737 DNA-markers were used in this study to identify the allelic type of the Rpv3 gene. The work was performed using the polymerase chain reaction. The reaction products were separated by capillary electrophoresis using the ABI Prism 3130 automatic genetic analyzer. Evaluation of the results was done using the GeneMapper and PeakScanner software. Functional alleles of the downy mildew resistance gene Rpv3 were revealed in grape varieties “Kishmish zaporozhskiy”, “Lady Patricia”, “Remaily seedless”, “Pamyati Smirnova” and “Shayan”. Rpv3299-279, one of the seven known haplotypes, was identified in all the varieties. The pedigree analysis of the studied varieties indicated that the parental forms – “Seyve Villard” and “Seibel” hybrids – are presumably the donors of the gene. Grape accessions with the identified Rpv3 gene can be used in seedless varieties breeding as donors of resistance to downy mildew.

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SCREENING TECHNIQUES FOR DOWNY MILDEW RESISTANCE IN GHERKIN CUCUMBERS

International Journal of Geomate ◽

10.21660/2017.40.2603 ◽

2017 ◽

Vol 13 (40) ◽

Author(s):

Piyavadee Charoenwattana

Keyword(s):

Downy Mildew ◽

Downy Mildew Resistance ◽

Mildew Resistance ◽

Screening Techniques

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High resolution mapping and candidate gene identification of downy mildew race 16 resistance in spinach

BMC Genomics ◽

10.1186/s12864-021-07788-8 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Gehendra Bhattarai ◽

Wei Yang ◽

Ainong Shi ◽

Chunda Feng ◽

Braham Dhillon ◽

...

Keyword(s):

Candidate Genes ◽

Downy Mildew ◽

Association Analysis ◽

Spinacia Oleracea ◽

Genotyping By Sequencing ◽

Significant Snps ◽

Snp Markers ◽

Resistance Locus ◽

Downy Mildew Resistance ◽

Mildew Resistance

Abstract Background Downy mildew, the most devastating disease of spinach (Spinacia oleracea L.), is caused by the oomycete Peronospora effusa [=P. farinosa f. sp. spinaciae]. The P. effusa shows race specificities to the resistant host and comprises 19 reported races and many novel isolates. Sixteen new P. effusa races were identified during the past three decades, and the new pathogen races are continually overcoming the genetic resistances used in commercial cultivars. A spinach breeding population derived from the cross between cultivars Whale and Lazio was inoculated with P. effusa race 16 in an environment-controlled facility; disease response was recorded and genotyped using genotyping by sequencing (GBS). The main objective of this study was to identify resistance-associated single nucleotide polymorphism (SNP) markers from the cultivar Whale against the P. effusa race 16. Results Association analysis conducted using GBS markers identified six significant SNPs (S3_658,306, S3_692697, S3_1050601, S3_1227787, S3_1227802, S3_1231197). The downy mildew resistance locus from cultivar Whale was mapped to a 0.57 Mb region on chromosome 3, including four disease resistance candidate genes (Spo12736, Spo12784, Spo12908, and Spo12821) within 2.69–11.28 Kb of the peak SNP. Conclusions Genomewide association analysis approach was used to map the P. effusa race 16 resistance loci and identify associated SNP markers and the candidate genes. The results from this study could be valuable in understanding the genetic basis of downy mildew resistance, and the SNP marker will be useful in spinach breeding to select resistant lines.

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Mutants of downy mildew resistance in Lactuca sativa (lettuce).

Genetics ◽

10.1093/genetics/137.3.867 ◽

1994 ◽

Vol 137 (3) ◽

pp. 867-874

Author(s):

P A Okubara ◽

P A Anderson ◽

O E Ochoa ◽

R W Michelmore

Keyword(s):

Molecular Marker ◽

Downy Mildew ◽

Genomic Dna ◽

Spontaneous Mutation ◽

Multigene Families ◽

Downy Mildew Resistance ◽

Bremia Lactucae ◽

Wild Type ◽

Mildew Resistance ◽

Recessive Mutations

Abstract As part of our investigation of disease resistance in lettuce, we generated mutants that have lost resistance to Bremia lactucae, the casual fungus of downy mildew. Using a rapid and reliable screen, we identified 16 distinct mutants of Latuca sativa that have lost activity of one of four different downy mildew resistance genes (Dm). In all mutants, only a single Dm specificity was affected. Genetic analysis indicated that the lesions segregated as single, recessive mutations at the Dm loci. Dm3 was inactivated in nine of the mutants. One of five Dm 1 mutants was selected from a population of untreated seeds and therefore carried a spontaneous mutation. All other Dm1, Dm3, Dm5/8 and Dm7 mutants were derived from gamma- or fast neutron-irradiated seed. In two separate Dm 1 mutants and in each of the eight Dm3 mutants analyzed, at least one closely linked molecular marker was absent. Also, high molecular weight genomic DNA fragments that hybridized to a tightly linked molecular marker in wild type were either missing entirely or were truncated in two of the Dm3 mutants, providing additional evidence that deletions had occurred in these mutants. Absence of mutations at loci epistatic to the Dm genes suggested that such loci were either members of multigene families, were critical for plant survival, or encoded components of duplicated pathways for resistance; alternatively, the genes determining downy mildew resistance might be limited to the Dm loci.

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