scholarly journals The Effect of Melinjo Peel Extract in Activity of Lipoprotein Lipase Enzym of The Rats Fed a Hypercholesterolemia Diet

2018 ◽  
Vol 7 (2) ◽  
pp. 133
Author(s):  
Athira Demitri
Keyword(s):  
Enzyme Activity ◽  
Statistical Analysis ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Diet Group ◽  
Lipoprotein Lipase Activity ◽  
Oral Gavage ◽  
Hypercholesterolemic Diet ◽  
Before And After ◽  
Peel Extract

The intake of saturated fat and cholesterol that comes from food digested in the intestine resulting in free fatty acids, triglycerides, phospholipids, and cholesterol. Triglyceride levels increased can be caused by presence of impaired lipoprotein lipase (LPL) enzyme activity. LPL activity can be increased by flavonoid in plant, like Melinjo peel. The purpose of this study is to measure LPL enzyme activity before and after being given extract of Melinjo peel treatment. This research use true experimental, with study design of pretest posttest control group design. Hypercholesterolemic diet given to rats by oral gavage as much as 1,8 grams for 14 days. Melinjo peel extract were given by oral gavage for 14 days after the hypercholesterolemic diet is given. Statistical analysis used Paired Sample T-Test to compare lipoprotein lipase activity before and after treatment. Then, used MANOVA (Multivariate Analyses of Variance) to see the difference of lipoprotein lipase activity in each group after treatment.Based on statistical analysis showed that there were differences of lipoprotein lipase activity in the hypercholesterolemic diet group, HD+54.15, HD+108.30, and HD+216.60 before and after treatment significant (p. < 0.05). The activity of lipoprotein lipase in the normal diet group compared with the hypercholesterolemic and HD+216.60 group showed a significant difference (p. < 0.05). Melinjo peel extract can increased activity of lipoprotein lipase enzym after treatment, those can be due to the flavonoid in Melinjo peel extract.

Refeeding and insulin increase lipoprotein lipase activity in rat brown adipose tissue

1989 ◽  
Vol 256 (5) ◽  
pp. E645-E650 ◽  
Author(s):  
C. M. Carneheim ◽  
S. E. Alexson
Keyword(s):  
Enzyme Activity ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Starvation Period ◽  
Lipoprotein Lipase Activity ◽  
Mrna Synthesis ◽  
Beta Adrenergic ◽  
Brown Adipose

Induction of lipoprotein lipase activity in brown adipose tissue (BAT) in response to cold stress has earlier been shown to be regulated by a beta-adrenergic mechanism and to be dependent on mRNA synthesis. In the present study, we have investigated the acute effects of refeeding after a short starvation period and the hormonal mechanism underlying the observed effects. Refeeding was found to rapidly increase tissue wet weight and lipoprotein lipase activity. The increase in enzyme activity could be blocked by the RNA synthesis inhibitor actinomycin D, indicating a gene activation. beta-Adrenergic blockade had no effect on this elevation of enzyme activity, but the increase could be mimicked by insulin injection. The results suggest that BAT contains two different pathways for regulation of lipoprotein lipase activity, both involving mRNA synthesis.

Plasma Lipoprotein Lipase in Children with Idiopathic Nephrotic Syndrome

PEDIATRICS ◽  
1969 ◽  
Vol 44 (6) ◽  
pp. 1021-1024
Author(s):  
Lawrence R. Hyman ◽  
Paul W. K. Wong ◽  
Aaron Grossman
Keyword(s):  
Enzyme Activity ◽  
Nephrotic Syndrome ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Idiopathic Nephrotic Syndrome ◽  
Plasma Lipoprotein ◽  
Lipoprotein Lipase Activity ◽  
The Mean

Plasma lipoprotein lipase activity was determined in six children with idiopathic nephrotic syndrome and in six controls. The mean lipoprotein lipase activity was significantly lower in the symptomatic nephrotic patients. This decreased enzyme activity may contribute to the hyperlipemia usually observed in the nephrotic syndrome. See table in the PDF file

scholarly journals Changes in the lipoprotein lipase (clearing-factor lipase) activity of white adipose tissue during development of the rat

1978 ◽  
Vol 172 (2) ◽  
pp. 319-325 ◽  
Author(s):  
A Cryer ◽  
H M Jones
Keyword(s):  
Enzyme Activity ◽  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
White Adipose Tissue ◽  
Lipase Activity ◽  
Plasma Insulin ◽  
Serum Concentrations ◽  
Lipoprotein Lipase Activity ◽  
Activity Change ◽  
Adipose Tissue Lipoprotein Lipase

The lipoprotein lipase (clearing-factor lipase) activity of the white adipose tissue from rats aged between 1 and 145 days was determined. Five adipose-tissue sites (epididymal, uterine, subcutaneous, perirenal and intramuscular) together with serum concentrations of triacylglycerol, cholesterol and glucose were studied. The pattern of enzyme-activity change was remarkably similar in all the sites studied, although the growth of the tissues proceeded non-uniformly. After a peak of activity early in suckling, lipoprotein lipase activity fell to low values by 20 days of age. At weaning (21 days) the activity increased sharply and within 5 days high values were regained. The serum triacylglycerol and cholesterol concentrations were low at birth and reached peaks of concentration coincidentally with the minima of white-adipose-tissue lipoprotein lipase activities, seen late in suckling. The changes in enzyme activity were related to other metabolic changes in adipose tissue and with the known changes in plasma insulin concentrations occurring during development.

scholarly journals Lipoprotein lipase activity in the tissues of guinea pigs exposed to different dietary fats from conception to three months of age

1978 ◽  
Vol 170 (1) ◽  
pp. 169-172 ◽  
Author(s):  
A Cryer ◽  
J Kirtland ◽  
H M Jones ◽  
M I Gurr
Keyword(s):  
Skeletal Muscle ◽  
Enzyme Activity ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Guinea Pigs ◽  
Dietary Fats ◽  
Lipoprotein Lipase Activity ◽  
Adipose Tissue Lipoprotein Lipase ◽  
Maize Oil

Long-term exposure of guinea pigs to a diet rich in maize oil caused an increase in adipose-tissue lipoprotein lipase activity. A similar diet rich in beef tallow had no such effect, and neither diet affected the enzyme activity in heart, lung, diaphragm or skeletal muscle.

scholarly journals Regulation of lipoprotein lipase activity and mRNA in the mammary gland of the lactating mouse

1994 ◽  
Vol 298 (2) ◽  
pp. 321-327 ◽  
Author(s):  
D R Jensen ◽  
S Gavigan ◽  
V Sawicki ◽  
D L Witsell ◽  
R H Eckel ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Adipose Tissue ◽  
Mammary Gland ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Physiological State ◽  
Mouse Mammary Gland ◽  
Reproductive Stage ◽  
Lipoprotein Lipase Activity ◽  
Translational Level

We examined the effects of reproductive stage and fasting on lipoprotein lipase (LPL) activity and mRNA in the mouse mammary gland. Heparin-releasable and cell-associated LPL activity rose immediately after birth, followed 1-2 days later by an increase in LPL mRNA. Fasting decreased LPL activity in the mammary gland at all reproductive stages. During lactation, both milk and heparin-releasable LPL were substantially decreased by an overnight fast, whereas cell-associated LPL was less affected and LPL mRNA did not change. These studies indicate that the extracellular, heparin-releasable, fraction of mammary LPL activity responds most rapidly to alterations in physiological state, usually accompanied by smaller changes in cellular enzyme activity. Changes in the level of LPL mRNA were seen only during the transition from pregnancy to lactation, and these tended to follow, rather than precede, changes in enzyme activity. We conclude that in the mammary gland as in adipose tissue, LPL is regulated primarily at the translational and post-translational level.

Comparative effects of insulin and isoproterenol on lipoprotein lipase in rat adipose cells

1996 ◽  
Vol 270 (5) ◽  
pp. C1461-C1467 ◽  
Author(s):  
G. E. Chiappe de Cingalani ◽  
J. W. Goers ◽  
M. Giannotti ◽  
C. I. Caldiz
Keyword(s):  
Enzyme Activity ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Incubation Medium ◽  
Cell Membranes ◽  
Lipoprotein Lipase Activity ◽  
Rat Adipocytes ◽  
Enzyme Degradation ◽  
Cell Incubation ◽  
Adipose Cells

The effects of insulin and isoproterenol on lipoprotein lipase mass and enzyme activity were investigated in rat adipocytes. Cells were pulse labeled for 1 h with [35S]methionine to measure immunoprecipitable lipoprotein lipase. The results showed that 80% of the newly synthesized enzyme was membrane associated and 20% was secreted into the cell incubation medium. Enzyme activity was mainly associated with lipoprotein lipase secreted into the medium. A 10-min incubation with 10(-7) M insulin stimulated the secretion of lipoprotein lipase activity and the activity associated with adipocyte membranes. Conversely, 10(-6) M isoproterenol decreased the activity in all fractions. In addition, insulin increased lipoprotein lipase mass associated with cell membranes and decreased that in the incubation medium, whereas isoproterenol induced a decrease in both cell membranes and medium. Insulin and isoproterenol stimulated phosphorylation of lipoprotein lipase. These findings suggest that insulin stimulates the secretion of active lipoprotein lipase and a reuptake of inactive secreted enzyme, and isoproterenol decreases the activity by enzyme degradation. Moreover, because both agents stimulate phosphorylation of lipoprotein lipase, phosphorylation may play a role in the effect of insulin increasing enzyme activity, in secretion or reuptake, and in the effect of isoproterenol inducing degradation of lipoprotein lipase.

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