scholarly journals ACTIVITIES OF AMYLASE, PROTEASE and LIPASE FROM HONEY Trigona sp, Apis mellifera and Apis dorsata

2018 ◽  
Vol 16 (1) ◽  
pp. 27
Author(s):  
Hendric Surya Putra ◽  
Winni Astuti ◽  
Rudi Kartika
Keyword(s):  
Salicylic Acid ◽  
Apis Mellifera ◽  
Lipase Activity ◽  
Protein Concentration ◽  
Coconut Oil ◽  
Apis Dorsata ◽  
Acid Base ◽  
Acid Base Titration ◽  
Bradford Method

Activities of amylase, protease and lipase from honey Trigona sp, Apis mellifera and Apis dorsata, determination protein concentration and the activity protease done with Bradford method, the determination of the glucose standard and activity amylase done with 3,5-dinitro salicylic acid (DNS) method and activity lipase done with acid-base titration with coconut oil substrate. The honey from Trigona sp has value of the activity amylase and lipase respectively by 0,0136 U / mg and 0,359 U/mg, whereas honey Apis mellifera has activity of protease, amylase and lipase of each 1,22 x 10-6 U/mg; 0,944 U/mg and 0,304 U/mg and then honey Apis dorsata has amylase and lipase activity of each of 0,0645 U/mg and 0,287 U/mg.

Determination Methods of Dialdehyde Celluloses from Bacterial Cellulose

2007 ◽  
Vol 361-363 ◽  
pp. 507-510
Author(s):  
Yu Hong Feng ◽  
Jia Cheng Li ◽  
Qiang Lin ◽  
Su Juan Pang ◽  
Meng Ying Zhang ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Amino Nitrogen ◽  
Aldehyde Group ◽  
Acid Base ◽  
Titration Method ◽  
Cannizzaro Reaction ◽  
Acid Base Titration ◽  
Determination Methods ◽  
Oxidation System

This paper addresses the determination of aldehyde group (% of dialdehyde units) in dialdehyde cellulose (DACs) oxidized from bacterial cellulose (BC). With 4 determination methods, the oxidation degrees of DACs were studied: Automatic potential titration method through Schiff base reaction, Acid-base titration in Cannizzaro reaction, Metaperiodate consumption determination, and Measurement of amino nitrogen content in DAC derivatives. By comparing determination results of these methods, we found out the proper way suitable for this metaperiodate oxidation system of BC to measure the dialdehyde unit content of the DACs.

scholarly journals Management of Achatina fulica (Bowdich, 1822) (Pulmonata: Achatinidae) in lettuce (Lactuca sativa L.)

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Lindinalva Santos ◽  
Carla Barbosa Negrisoli ◽  
Maciel Bispo Santos ◽  
Aldomario Negrisoli Junior
Keyword(s):  
Enzymatic Activity ◽  
Lipase Activity ◽  
Protein Concentration ◽  
Lethal Dose ◽  
Alcoholic Extract ◽  
Achatina Fulica ◽  
Before And After ◽  
Important Pest ◽  
Lethal Time

ABSTRACT: The giant African snail Achatina fulica was introduced in Brazil and since then has become an important pest, because of its resistance to abiotic conditions, hermaphroditism, polyphagia, and absence of natural predators. This study aims to evaluate the control of A. fulica in lettuce, in Alagoas, Brazil. Bioassays for the determination of lethal dose and lethal time to adults of A. fulica and the egg mortality were performed in the laboratory by applying commercial synthetic products, commercial and non-commercial alcoholic botanical extracts on mollusk adults. Additionally, the protein concentration, lipase activity and enzyme acetylcholinesterase (AChE), and butyrylcholinesterase (BuChE), in the stomach, intestine, nervous ganglion and liver were determined. The alcoholic extract of Capsicum frutescens caused higher mortality of A. fulica, and the alcoholic extract of C. frutescens and Piper tuberculatum oil can prevent the hatching of A. fulica. The lipase activity was present and in greater quantities in tissues, stomach, intestine, liver and ganglia of A. fulica, before and after exposure of the alcoholic extract of C. frutescens. The enzymatic activity of BuChE was present in the ganglia and liver of A. fulica, prior to exposure of the alcoholic extract of C. frutescens. The enzymatic activity of AChE was present only in the ganglion and absent in liver of A. fulica, prior to exposure of the alcoholic extract of C. frutescens. The concentration of 10% of the alcoholic extract of C. frutescens caused 84% mortality of adult A. fulica in lettuce in field conditions.

scholarly journals Electrometric Determination Of Binary Mixtures In Food

2021 ◽  
Vol 03 (06) ◽  
pp. 8-13
Author(s):  
Narbek Sapaevich Palvanov ◽  
◽  
Mukhiddin Sultanovich Mekhmanov ◽  
Keyword(s):  
Aqueous Solutions ◽  
Binary Mixtures ◽  
Dicarboxylic Acids ◽  
Potentiometric Determination ◽  
Acid Base ◽  
Acid Base Titration

In this paper, the possibility of acid-base titration of binary mixtures of dicarboxylic acids in mixed and non-aqueous solutions is investigated. The possibility of titrimetric potentiometric determination of binary mixtures of dicarboxylic acids is shown.

scholarly journals USING POTENTIOMETRIC ACID-BASE TITRATION TO DETERMINE PKA FROM MANGOSTEEN PERICARPS EXTRACT

2019 ◽  
Vol 16 (32) ◽  
pp. 768-773
Author(s):  
L. TANIA ◽  
C. DIAWATI ◽  
M. SETYARINI ◽  
N. KADARITNA ◽  
A. SAPUTRA
Keyword(s):  
Oxalic Acid ◽  
Potentiometric Titration ◽  
Accurate Determination ◽  
Acidity Constant ◽  
Equivalence Point ◽  
Acid Base ◽  
Titrant Volume ◽  
Acid Base Titration ◽  
Ph Range

One of the uses of acid-base indicators is to show the end point of the titration, so the accurate determination of acidity constant and pH range of indicators needs to be done. This study aims to determine the acidity constant (pKa) of mangosteen pericarp extract and its accuracy as an indicator of acid-base titration. Determination of pKa was done by a simple potentiometric titration method. The titration data were plotted in three graphs, i.e., pH, ΔpH/ΔV (the (the first derivative), and Δ2pH/ΔV2 (the second derivative) versus titrant volume to determine the equivalence point of the titration. The accuracy test was carried out by comparing the volume of oxalic acid used to titrate NaOH solution using the indicator of mangosteen pericarp extract and phenolphthalein indicator. The result showed that the equivalence point was found on the titrant volume of 8.6 mL and a measured pH of 9.84. so the pKa value of mangosteen pericarp extract was 7.20, and the pH range was 6.20 to 8.20. the average volume of oxalic acid used to titrate 5 mL of NaOH using phenolphthalein as the indicator was 5.2 mL while the titration used mangosteen pericarp extract was 5.23 mL. The accuracy of mangosteen pericarp extract was 99.42%. By the result, it can be concluded that potentiometric titration can be used as a simple way to determine the acidity constant of mangosteen pericarp extract. Moreover, the mangosteen pericarp extract can be used as an alternative acid-base titration indicator to substitute the common acid-base titration in the laboratory.

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