scholarly journals SEED QUALITY EVALUATION IN THE COLLECTION OF CYAMOPSIS TETRAGONOLOBA AFTER LONG¬TERM STORAGE UNDER UNCONTROLLED CONDITIONS

2020 ◽  
Vol 180 (4) ◽  
pp. 48-58
Author(s):  
N. V. Rakovskaya ◽  
O. N. Zabegayeva ◽  
E. A. Dzyubenko
Keyword(s):  
Seed Quality ◽  
Seed Viability ◽  
Growing Season ◽  
Cyamopsis Tetragonoloba ◽  
Monthly Temperature ◽  
Germination Energy ◽  
Long Term Storage ◽  
Seed Hardness ◽  
Term Storage

Background. Guar or clusterbean (Cyamopsis tetragonoloba (L.) Taub.) is an annual leguminous plant cultivated for feed, food and industrial purposes. Its seed endosperm is used to extract guar gum, so the guar genetic resources stored in the VIR collection are of strategic importance for the import substitution policy in Russia in the context of their prospective introduction into cultivation in the country’s southern areas. Most of the guar accessions had been preserved by VIR for 40 years or more, so the task was to assess their germination rates and restore their viability. In the process of screening, seed quality parameters of the accessions were examined and analyzed.Materials and methods. VIR’s collection of C. tetragonoloba, initiated by N. I. Vavilov, now contains 111 accessions. Percentage seed viability, germination energy and seed hardness were evaluated in the Long-Term Storage Laboratory for 50 accessions reproduced in 2018 and for 263 older reproductions of 89 guar accessions. In total, 313 seed samples reproduced across 18 different years were analyzed. Statistica 10 software was used to perform a single-factor analysis of variance and find out how germination energy, seed viability and seed hardness correlated with the age of seeds, mean monthly temperature and mean rainfall during the growing season.Results and discussion. After more than 40 years of storage, the guar accessions did not entirely lost their viability under laboratory and field conditions. Very high positive correlations were found for their percentage viability and germination energy with the mean monthly temperature in the year of reproduction. A weak positive correlation was observed between seed hardness and the year of reproduction. Correlations between seed viability and rainfall during the growing season were weak, with a weak negative correlation between seed hardness and rainfall. The viability level of 30-year-old seeds was 50%, so guar may be regarded as belonging to the group of macrobiotic plants.

scholarly journals Barley Seeds miRNome Stability during Long-Term Storage and Aging

2021 ◽  
Vol 22 (9) ◽  
pp. 4315
Author(s):  
Marta Puchta ◽  
Jolanta Groszyk ◽  
Magdalena Małecka ◽  
Marek D. Koter ◽  
Maciej Niedzielski ◽  
...  
Keyword(s):  
Seed Viability ◽  
Small Rna Sequencing ◽  
The Novel ◽  
Seed Aging ◽  
Long Term Storage ◽  
Novel Mirna ◽  
Complex Biological Process ◽  
Almost All ◽  
Term Storage

Seed aging is a complex biological process that has been attracting scientists’ attention for many years. High-throughput small RNA sequencing was applied to examine microRNAs contribution in barley seeds senescence. Unique samples of seeds that, despite having the same genetic makeup, differed in viability after over 45 years of storage in a dry state were investigated. In total, 61 known and 81 novel miRNA were identified in dry seeds. The highest level of expression was found in four conserved miRNA families, i.e., miR159, miR156, miR166, and miR168. However, the most astonishing result was the lack of significant differences in the level of almost all miRNAs in seed samples with significantly different viability. This result reveals that miRNAs in dry seeds are extremely stable. This is also the first identified RNA fraction that is not deteriorating along with the loss of seed viability. Moreover, the novel miRNA hvu-new41, with higher expression in seeds with the lowest viability as detected by RT-qPCR, has the potential to become an indicator of the decreasing viability of seeds during storage in a dry state.

scholarly journals Development of cryopreservation methods of seed of Nepeta cataria

2021 ◽  
Vol 102 (2) ◽  
pp. 37-42
Author(s):  
Margarita Ishmuratova ◽  
◽  
Damirzhan Baigarayev ◽  
Saltanat Tleukenova ◽  
Elena Gavrilkova ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Room Temperature ◽  
Seed Viability ◽  
Positive Temperature ◽  
Seed Rate ◽  
Nepeta Cataria ◽  
Long Term Storage ◽  
Medical Plant ◽  
Term Storage

This article presents the summarized data on cryopreservation of seeds of the medical plant Nepeta cataria. Cryopreservation is a highly promising method for saving of seed materials, allowing to organize long-term storage without viability loss. The purpose of present work is to optimize conditions of cryopreservation of seed materials of Nepeta cataria. Assessment of seed survival rate in the storage showed a linear decrease in seed viability and energy of germination. After 30 months of storage at the low positive temperature (+5 ºC) in paper pack seed rate decreased to 12.0 % and energy of germination to 11.2 %; after 4 years of storage seeds lost viability. During conduction of research the type of container, condition of thawing, optimal moisture of seeds and cryoprotectants are optimized. The optimal container for cryopreservation in liquid nitrogen was plastic cryo tubes; defrosting at room temperature. The best seed rate is found at moisture 3 %; the best cryoprotectant was glucose, the optimal concentration was 15 %. The result of the research is used for creation of the long-term storage medicinal cultures’ seed bank in the liquid nitrogen.

The effect of prolonged storage and periodic reproduction on the germination of red beet seeds

2020 ◽  
pp. 35-37
Author(s):  
В.Д. Богданова ◽  
М.В. Воробьев
Keyword(s):  
Shelf Life ◽  
Cultivated Plants ◽  
Seed Formation ◽  
Germination Energy ◽  
Long Term Storage ◽  
Red Beet ◽  
Duration Of Storage ◽  
Field Germination ◽  
Term Storage

Площади под свеклой в Российской Федерации колеблются в пределах 65-68 тыс. га, что составляет 7% от общей площади, занятой овощными культурами. Вопросы, связанные с продолжительностью хранения и периодического воспроизведения на всхожесть семян остаются актуальными. В работе исследованы изменения таких важнейших посевных качеств, как энергия прорастания и всхожесть семян столовой свеклы на примере сорта Двусемянная ТСХА после различных сроков хранения. Благодаря селекционной работе можно значительно увеличить сроки хозяйственной годности семян и продолжительность их биологической жизнеспособности. Цель исследований: оценка влияния сроков хранения семян столовой свеклы сорта Двусемянная ТСХА на их посевные качества (всхожесть и энергия прорастания). Для этого определяли всхожесть и энергию прорастания семян столовой свеклы различных семей сорта Двусемянная ТСХА в условиях длительного хранения, изучили полевую всхожесть семян в условиях длительного хранения, установили оптимальные сроки хранения. Полевая всхожесть отдельных семей существенно отличалась. В некоторых случаях, особенно после продолжительного хранения, разница достигала 70%. Наглядно показано, что энергия прорастания и всхожесть семян слабо изменяются в течение четырех лет при отсутствии различных обработок. Выявлено влияние погодных условий в год выращивания семян и генотипических различий семей, что рекомендуется учитывать в селекции и при производстве элитных семян. Результаты исследования свидетельствуют о высокой биологической долговечности семян свеклы (более 16 лет), даже при хранении в комнатных условиях. Это важно при репродукции, так как в результате потепления климата изменяется направленность отбора в сторону более позднеспелых форм, менее перерастающих и устойчивых к растрескиванию и деформации корнеплодов. Высокое качество семенного материала культурных растений зависит от условий формирования семян. The area under red beet in the Russian Federation ranges from 65-68 thousand ha, which is 7% of the total area occupied by vegetables. Questions related to the duration of storage and periodic reproduction for seed germination are relevant. The work investigated the changes in such important sowing qualities as the germination energy and germination of red beet seeds using the example of the Dvusemyannaya TSKHA variety, after different storage periods. Thanks to the conduct of breeding work, it is possible to significantly increase the terms of economic shelf life of seeds and the duration of their biological viability. The aim of the study was to assess the effect of the shelf life of seeds of red beet variety Dvusemyannaya TSKHA on their sowing qualities (germination and germination energy). To do this, we determined the germination and germination energy of seeds of red beet from different families of the variety Dvusemyannaya TSKHA under conditions of long-term storage, studied the field germination of seeds under conditions of long-term storage, and established the optimal storage periods. Field germination of individual families differed significantly. In some cases, especially as a result of long-term storage, the difference reached 70%. It is clearly shown that the energy of germination and germination of seeds change little over four years in the absence of various treatments. The influence of weather conditions in the year of seed cultivation and genotypic differences of families was revealed. It is recommended to take these factors into account in breeding and in the production of elite seeds. The research results presented by the author indicate a high biological longevity of beet seeds, even when stored in room conditions, which is more than 16 years. This is important for reproduction, since because of climate warming, the direction of selection changes towards later-maturing forms that are less outgrowing and resistant to cracking and deformation of root crops. The high quality of the seed material of cultivated plants depends on the conditions of seed formation.

scholarly journals Localization and Dynamics of the Methionine Sulfoxide Reductases MsrB1 and MsrB2 in Beech Seeds

2021 ◽  
Vol 22 (1) ◽  
pp. 402
Author(s):  
Natalia Wojciechowska ◽  
Agnieszka Bagniewska-Zadworna ◽  
Julia Minicka ◽  
Kornel M. Michalak ◽  
Ewa M. Kalemba
Keyword(s):  
Stress Responses ◽  
Management Practices ◽  
Seed Viability ◽  
Methionine Sulfoxide ◽  
Embryonic Axes ◽  
Long Term Storage ◽  
Protein Storage Vacuoles ◽  
Methionine Sulfoxide Reductases ◽  
Term Storage

Beech seeds are produced irregularly, and there is a need for long-term storage of these seeds for forest management practices. Accumulated reactive oxygen species broadly oxidize molecules, including amino acids, such as methionine, thereby contributing to decreased seed viability. Methionine oxidation can be reversed by the activity of methionine sulfoxide reductases (Msrs), which are enzymes involved in the regulation of many developmental processes and stress responses. Two types of Msrs, MsrB1 and MsrB2, were investigated in beech seeds to determine their abundance and localization. MsrB1 and MsrB2 were detected in the cortical cells and the outer area of the vascular cylinder of the embryonic axes as well as in the epidermis and parenchyma cells of cotyledons. The abundances of MsrB1 and MsrB2 decreased during long-term storage. Ultrastructural analyses have demonstrated the accumulation of these proteins in protein storage vacuoles and in the cytoplasm, especially in close proximity to the cell membrane. In silico predictions of possible Msr interactions supported our findings. In this study, we investigate the contribution of MsrB1 and MsrB2 locations in the regulation of seed viability and suggest that MsrB2 is linked with the longevity of beech seeds via association with proper utilization of storage material.

Response of Longleaf Pine Seeds to Storage Conditions and Pregermination Treatments

1993 ◽  
Vol 17 (4) ◽  
pp. 174-179 ◽  
Author(s):  
James P. Barnett ◽  
John P. Jones
Keyword(s):  
Seed Quality ◽  
Longleaf Pine ◽  
Pinus Palustris ◽  
Storage Conditions ◽  
Operational Conditions ◽  
Long Term Storage ◽  
Processing And Storage ◽  
And Storage ◽  
Term Storage

Abstract Although longleaf pine (Pinus palustris Mill.) seeds are considered the most susceptible of the southern pines to damage during collection, processing, and storage, results of these studies show that high seed quality can be assured for periods up to 20 yr through proper handling and storing techniques. Recommendations for long-term storage include drying seeds to moisture contents of 10% or less and storing at subfreezing temperatures, preferably near 0°F. Reevaluation of stratification treatments applied under operational conditions indicates that the soaking in water that is necessary for seed imbibition reduces total germination in an amount proportional to the length of the soak. Stratification is not recommended except under very controlled conditions. South. J. Appl. For. 17(4):174-179.

scholarly journals Chemical treatment of papaya seeds aiming at long-term storage and control of damping off

Revista CERES ◽  
2014 ◽  
Vol 61 (3) ◽  
pp. 384-391
Author(s):  
Silvia de Carvalho Campos Botelho ◽  
Silvaldo Felipe da Silveira ◽  
Roberto Ferreira da Silva ◽  
Alexandre Pio Viana
Keyword(s):  
Seed Quality ◽  
Seedling Emergence ◽  
Seed Vigor ◽  
Control Group ◽  
Damping Off ◽  
Effective Measure ◽  
Long Term Storage ◽  
Papaya Seeds ◽  
Term Storage

Damping off is a nursery disease of great economic importance in papaya and seed treatment may be an effective measure to control. The aim of this work was to evaluate the quality of papaya seeds treated with fungicides and stored under two environmental and packaging conditions. Additionally, the efficiency of fungicide treatments in the control of damping-off caused by Rhizoctonia solani was evaluated. Papaya seeds were treated with the fungicides Captan, Tolylfluanid and the mixture Tolylfluanid + Captan (all commercial wettable powder formulations). Seeds of the control group were not treated. The seeds were stored for nine months in two conditions: packed in aluminum coated paper and kept at 7 ± 1ºC and in permeable kraft paper and kept in non-controlled environment. At the beginning of the storage and every three months the seed quality (germination and vigor tests), emergence rate index, height, dry mass and damping of plants in pre and post-emergence (in contaminated substrate and mycelia-free substrate) were analyzed. Both storage conditions as well as the fungicide treatments preserved the germination and seed vigor. In the infested substrate, seedling emergence was favored by fungicides, but in post-emergence, fungicides alone did not control the damping off caused by R. solani. Symptoms of damping off were not observed in the clean substrate. The results showed that the fungicide treatments may be used to pretreat papaya seed for long-term storage and commercialization.

Sign in / Sign up

Export Citation Format

Share Document