scholarly journals Localization and Dynamics of the Methionine Sulfoxide Reductases MsrB1 and MsrB2 in Beech Seeds

2021 ◽  
Vol 22 (1) ◽  
pp. 402
Author(s):  
Natalia Wojciechowska ◽  
Agnieszka Bagniewska-Zadworna ◽  
Julia Minicka ◽  
Kornel M. Michalak ◽  
Ewa M. Kalemba
Keyword(s):  
Stress Responses ◽  
Management Practices ◽  
Seed Viability ◽  
Methionine Sulfoxide ◽  
Embryonic Axes ◽  
Long Term Storage ◽  
Protein Storage Vacuoles ◽  
Methionine Sulfoxide Reductases ◽  
Term Storage

Beech seeds are produced irregularly, and there is a need for long-term storage of these seeds for forest management practices. Accumulated reactive oxygen species broadly oxidize molecules, including amino acids, such as methionine, thereby contributing to decreased seed viability. Methionine oxidation can be reversed by the activity of methionine sulfoxide reductases (Msrs), which are enzymes involved in the regulation of many developmental processes and stress responses. Two types of Msrs, MsrB1 and MsrB2, were investigated in beech seeds to determine their abundance and localization. MsrB1 and MsrB2 were detected in the cortical cells and the outer area of the vascular cylinder of the embryonic axes as well as in the epidermis and parenchyma cells of cotyledons. The abundances of MsrB1 and MsrB2 decreased during long-term storage. Ultrastructural analyses have demonstrated the accumulation of these proteins in protein storage vacuoles and in the cytoplasm, especially in close proximity to the cell membrane. In silico predictions of possible Msr interactions supported our findings. In this study, we investigate the contribution of MsrB1 and MsrB2 locations in the regulation of seed viability and suggest that MsrB2 is linked with the longevity of beech seeds via association with proper utilization of storage material.

scholarly journals Barley Seeds miRNome Stability during Long-Term Storage and Aging

2021 ◽  
Vol 22 (9) ◽  
pp. 4315
Author(s):  
Marta Puchta ◽  
Jolanta Groszyk ◽  
Magdalena Małecka ◽  
Marek D. Koter ◽  
Maciej Niedzielski ◽  
...  
Keyword(s):  
Seed Viability ◽  
Small Rna Sequencing ◽  
The Novel ◽  
Seed Aging ◽  
Long Term Storage ◽  
Novel Mirna ◽  
Complex Biological Process ◽  
Almost All ◽  
Term Storage

Seed aging is a complex biological process that has been attracting scientists’ attention for many years. High-throughput small RNA sequencing was applied to examine microRNAs contribution in barley seeds senescence. Unique samples of seeds that, despite having the same genetic makeup, differed in viability after over 45 years of storage in a dry state were investigated. In total, 61 known and 81 novel miRNA were identified in dry seeds. The highest level of expression was found in four conserved miRNA families, i.e., miR159, miR156, miR166, and miR168. However, the most astonishing result was the lack of significant differences in the level of almost all miRNAs in seed samples with significantly different viability. This result reveals that miRNAs in dry seeds are extremely stable. This is also the first identified RNA fraction that is not deteriorating along with the loss of seed viability. Moreover, the novel miRNA hvu-new41, with higher expression in seeds with the lowest viability as detected by RT-qPCR, has the potential to become an indicator of the decreasing viability of seeds during storage in a dry state.

scholarly journals Development of cryopreservation methods of seed of Nepeta cataria

2021 ◽  
Vol 102 (2) ◽  
pp. 37-42
Author(s):  
Margarita Ishmuratova ◽  
◽  
Damirzhan Baigarayev ◽  
Saltanat Tleukenova ◽  
Elena Gavrilkova ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Room Temperature ◽  
Seed Viability ◽  
Positive Temperature ◽  
Seed Rate ◽  
Nepeta Cataria ◽  
Long Term Storage ◽  
Medical Plant ◽  
Term Storage

This article presents the summarized data on cryopreservation of seeds of the medical plant Nepeta cataria. Cryopreservation is a highly promising method for saving of seed materials, allowing to organize long-term storage without viability loss. The purpose of present work is to optimize conditions of cryopreservation of seed materials of Nepeta cataria. Assessment of seed survival rate in the storage showed a linear decrease in seed viability and energy of germination. After 30 months of storage at the low positive temperature (+5 ºC) in paper pack seed rate decreased to 12.0 % and energy of germination to 11.2 %; after 4 years of storage seeds lost viability. During conduction of research the type of container, condition of thawing, optimal moisture of seeds and cryoprotectants are optimized. The optimal container for cryopreservation in liquid nitrogen was plastic cryo tubes; defrosting at room temperature. The best seed rate is found at moisture 3 %; the best cryoprotectant was glucose, the optimal concentration was 15 %. The result of the research is used for creation of the long-term storage medicinal cultures’ seed bank in the liquid nitrogen.

scholarly journals Long term storage and the compensatory growth of white shrimp Litopenaeus vannamei in aquaculture ponds

2017 ◽  
Vol 44 (3) ◽  
pp. 588-594
Author(s):  
Geraldo Fóes ◽  
Dariano Krummenauer ◽  
Gabriele Lara ◽  
Luis Poersch ◽  
Wilson Wasielesky Jr.
Keyword(s):  
Compensatory Growth ◽  
Management Practices ◽  
Specific Growth ◽  
Survival Rates ◽  
White Shrimp ◽  
Growth And Survival ◽  
Conventional Management ◽  
Long Term Storage ◽  
Term Storage

Effects of shrimp confinement in a situation of high density stocking in a long term nursery on their growth performance in grow out ponds. Were analized two nurseries with a density of 2000 shrimp m-2 were stocked at two different times. The first nursery (LTN) lasted 144 days, and the SGR of the animals was 3.0% day-1. The second nursery (STN) lasted 18 days and the specific growth rate (SGR) was 19.9% day-1. On the same day, shrimps were transferred to six lined ponds at a density of 20 shrimp m2 where they remained for 101 days. In the first biometry, the SGR in the LTN treatment, increased to 6.7% day-1 and in the STN it decreased to 5.0% day-1. At the end, shrimps of the LTN and STN treatments reached weights of 8.46 and 6.72 g and had productivities of 1287 and 1015 kg ha-1, respectively. Shrimps reared in nurseries for long periods showed growth and survival rates similar to those obtained using conventional management practices in grow out structures.

scholarly journals SEED QUALITY EVALUATION IN THE COLLECTION OF CYAMOPSIS TETRAGONOLOBA AFTER LONG¬TERM STORAGE UNDER UNCONTROLLED CONDITIONS

2020 ◽  
Vol 180 (4) ◽  
pp. 48-58
Author(s):  
N. V. Rakovskaya ◽  
O. N. Zabegayeva ◽  
E. A. Dzyubenko
Keyword(s):  
Seed Quality ◽  
Seed Viability ◽  
Growing Season ◽  
Cyamopsis Tetragonoloba ◽  
Monthly Temperature ◽  
Germination Energy ◽  
Long Term Storage ◽  
Seed Hardness ◽  
Term Storage

Background. Guar or clusterbean (Cyamopsis tetragonoloba (L.) Taub.) is an annual leguminous plant cultivated for feed, food and industrial purposes. Its seed endosperm is used to extract guar gum, so the guar genetic resources stored in the VIR collection are of strategic importance for the import substitution policy in Russia in the context of their prospective introduction into cultivation in the country’s southern areas. Most of the guar accessions had been preserved by VIR for 40 years or more, so the task was to assess their germination rates and restore their viability. In the process of screening, seed quality parameters of the accessions were examined and analyzed.Materials and methods. VIR’s collection of C. tetragonoloba, initiated by N. I. Vavilov, now contains 111 accessions. Percentage seed viability, germination energy and seed hardness were evaluated in the Long-Term Storage Laboratory for 50 accessions reproduced in 2018 and for 263 older reproductions of 89 guar accessions. In total, 313 seed samples reproduced across 18 different years were analyzed. Statistica 10 software was used to perform a single-factor analysis of variance and find out how germination energy, seed viability and seed hardness correlated with the age of seeds, mean monthly temperature and mean rainfall during the growing season.Results and discussion. After more than 40 years of storage, the guar accessions did not entirely lost their viability under laboratory and field conditions. Very high positive correlations were found for their percentage viability and germination energy with the mean monthly temperature in the year of reproduction. A weak positive correlation was observed between seed hardness and the year of reproduction. Correlations between seed viability and rainfall during the growing season were weak, with a weak negative correlation between seed hardness and rainfall. The viability level of 30-year-old seeds was 50%, so guar may be regarded as belonging to the group of macrobiotic plants.

scholarly journals Does Storage under Gene Bank Conditions Affect Seed Germination and Seedling Growth? The Case of Senecio morisii (Asteraceae), a Vascular Plant Exclusive to Sardinian Water Meadows

Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 581 ◽  
Author(s):  
Alba Cuena-Lombraña ◽  
Martina Sanna ◽  
Marco Porceddu ◽  
Gianluigi Bacchetta
Keyword(s):  
Seedling Growth ◽  
Seedling Survival ◽  
Vascular Plant ◽  
Seed Viability ◽  
Storage Conditions ◽  
Growth And Survival ◽  
Survival Percentage ◽  
Long Term Storage ◽  
Term Storage

Understanding seed viability under long-term storage conditions provides basic and useful information to investigate the effectiveness of seed banking. Besides the germination success, seedling establishment is also an important requirement, and a decisive step to ensure plant propagation. We used comparative data of germination, seedling growth, and survival percentage between fresh and 10-years-stored seeds of Senecio morisii, a narrow endemic and vulnerable species of Sardinia (Italy), in order to evaluate if differences exist in these traits between fresh and 10-years-stored seeds. Stored seeds showed higher germination percentages than fresh ones, whereas seedling growth and survival did not present significant differences between them, except for seedling growth in plants produced from seeds germinated at 25 °C. This study allowed us to assess if seeds of S. morisii were able to germinate under controlled conditions, and if they maintained their viability and germination capacity for at least 10 years of long-term storage in the seed bank. In addition, the high seedling survival detected in both fresh and stored seeds suggests that stored seeds of S. morisii can be used to support reinforcement or reintroduction actions when fresh materials are not available.

scholarly journals Morphophysiology and Cryopreservation of Seeds of Dendrobium Nobile Lindl. (Orchidaceae) at Different Stages of Development

2021 ◽  
Author(s):  
Galina L Kolomeitseva ◽  
Tatiana V Nikishina ◽  
Alexander V Babosha ◽  
Andrey S Ryabchenko ◽  
Olga N Vysotskaya
Keyword(s):  
Seed Viability ◽  
Embryo Sac ◽  
Dendrobium Nobile ◽  
Long Term Storage ◽  
Immature Seeds ◽  
Dendrobium Nobile Lindl ◽  
Term Storage ◽  
Pod Culture

Abstract We studied the seed maturation of Dendrobium nobile Lindl to establish the optimal stage for green pod culture and long-term storage in liquid nitrogen. It was found that the immature seeds can germinate in vitro starting from 3 months after pollination (MAP) but only develop up to the stage of embryo swelling or protocorm without rhizoids. The maximum staining of embryos with vital dyes FDA and TTC occurs at 4 and 5 MAP that corresponds to the release of suspensor beyond the embryo sac. Embryo staining does not correlate with germination capacity and seed viability in cryogenic storage. The immature seeds acquire resistance to freezing and drying in airflow at the age of 6 MAP. The time of technical maturity for green pod culture and cryopreservation may be reduced from 12 MAP to 6–7 MAP.

Cryopreservation of Fagus crenata seeds: estimation of optimum moisture content for maintenance of seed viability by Bayesian modeling

2018 ◽  
Vol 48 (2) ◽  
pp. 192-196 ◽  
Author(s):  
Keita Endoh ◽  
Michinari Matsushita ◽  
Megumi K. Kimura ◽  
So Hanaoka ◽  
Yuko Kurita ◽  
...  
Keyword(s):  
Moisture Content ◽  
Seed Viability ◽  
Fagus Crenata ◽  
Natural Forests ◽  
Fagus Crenata Blume ◽  
Long Term Storage ◽  
High Viability ◽  
Term Storage ◽  
Seed Cryopreservation

Fagus crenata Blume is an important forestry tree species that plays a key biological role in natural forests. Because F. crenata forests are declining under global warming, the conservation of seed resources is necessary to maintain sustainable regeneration. We examined the capability of seed cryopreservation to achieve semi-permanent preservation of the F. crenata genetic resource. Seeds at 7%–26% moisture content (MC) exhibited greater than 64% viability, with developing radicles growing at 22 °C in 16 h photoperiods following chilling at 2 °C for 2 months. These seeds were then stored cryogenically at –170 °C for 6 months; cryopreservation at 26% MC reduced seed viability to 12%, whereas dry seeds (7%–14% MC) maintained viability and were comparable with seeds that had neither been dried nor cryopreserved. Drying seeds to 6% MC reduced viability to 47%; following cryopreservation, the viability of these seeds with such a low MC was 36%. Using a Bayesian model, we then determined that the optimal range of MC for the cryopreservation of F. crenata seeds was 9%–12%. We conclude that cryopreservation is feasible for the long-term storage of F. crenata seeds, drying of the seeds to the range of optimal MC retains high viability during cryogenic storage.

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