scholarly journals TRICHINELLOSIS OF THE STONE MARTEN (MARTES FOINA) IN CENTRAL RUSSIA

2021 ◽  
pp. 56-60
Author(s):  
Andreyanov

Over the past 5 years, research has been carried out in natural biocenosis on the stone marten (Martes foina) infected with the pathogen of trichinellosis in Central Russia. Biological material for research was collected in hunting farms, reserves and "green zones" of the Central Region of Russia (the Vladimir, Moscow, Nizhny Novgorod, Tula, Ryazan, Oryol and Bryansk regions) during the opening of amateur sports hunting for fur animals from 2017 to 2021. Diagnostics and isolation of helminth larvae from animal muscle tissue samples were performed by the compressor trichinelloscopy and digestion in artificial gastric juice. The degree of host invasion with helminth and invasion intensity were determined. A total of 35 animals were studied. As a result of studies, the infection rate of helminthozoonosis in animals was from 8.3 to 40.0%. The intensity of invasion was recorded between 2 and 39 larvae in 1 g of diaphragm muscle tissue. Trichinella invasion among martens was observed equally in both males and females. The largest percentage of the invasive form of the helminthiasis pathogen occurred in animals older than 2 years. The adverse situation of helminthozoonosis among fur animals was noted in the Ryazan region.

2020 ◽  
pp. 21-27
Author(s):  
Andreyanov

The spread of alariosis of fishing animals of Central Russia was clarified and a circle of host agents among mammals was identified. The material for the study was the arcasses of trade animals. The period of work was 2018–2020. Animals were studied according to the method of complete helminthological study on K.I. Skryabin (1928). The tissue form of alariosis was examined by trichinelloscopic methods. During the reporting period, 107 heads of carnivorous animals (of which 27 heads (25.2%) are considered stray domestic) extracted in the territory of the Ryazan, Vladimir and Moscow regions. Of the Canidae family, 54 heads have undergone helminthological examination (29 foxes, 17 domestic dogs, 7 raccoon dogs, and 1 wolf). Of the cat (Felidae), 11 heads have been studied (domestic cats – 10, lynx – 1). Martens animals (Mustelinae) have been studied 42 heads (forest мartens – 19, stone мartens – 6, river otter – 4, ermines – 7, badgers – 2, аmerican mink – 4). Adult trematodes have been identified in 37 carnivorous animals (34.6%) in the thin part of the intestine. Canidae animals representing trade species (fox, raccoon dog, wolf) are 100% infected with the agent of alariosis. The intensity invasion of fox was 4–250 copies per head, raccoon dogs – 11–284, wolf – 84. The larval form of the agent was recorded in one fox (3.5%), 2 forest мartens (10.5%) and one аmerican mink (25.0%). Mesocercaries of trematodes were localized in a transparent capsule in lung parenchyma, in diaphragm muscle tissue, in esophageal muscle tissue.


Author(s):  
N.S. Konoval

The issue on estimating the time of death coming still remains a challenge that requires a lot of questions to be answered, therefore, the development of new scientific methods aimed at overcoming these difficulties is a top priority in solving the problem of estimating the time of death coming. The purpose of this study was to investigate the structural and biochemical changes in the muscle tissue of the diaphragm in the early post-mortem period. The muscular tissue samples of the diaphragm just below the lungs taken from 30 human corpses in the early post-mortem period (in time intervals of 3-13 hours from the onset of death) were investigated. We studied structural and biochemical markers in the homogenates of the muscles of the diaphragm: the content of glycogen, acid phosphatase, lactate, lactate dehydrogenase, lipofuscin, and cholinesterase. It was found out that in the early post-mortem period structural and biochemical changes in the muscle tissue of the diaphragm are characterized by regular morphological biochemical and biophysical changes. In particular, the post-mortem dynamic changes in glycogen, acid phosphatase and lipofuscin levels is most significant; i.e. within the time interval of 3–9 hours from the time of death coming, the assessment of the “cholinesterase / glycogen or acidic phosphatase / glycogen” ratio is the most diagnostically valuable, then after 9 hours the only “cholinesterase / glycogen” ratio is meaningful. The content of glycogen in the homogenates of the diaphragm muscle tissue depending on the time of death coming ranged from (4.765 ± 0.058) mg / g in 3 hours, to (1.883 ± 0.027) mg / g in 13 hours after the death coming, reliably ( p <0.001) differing in time intervals. The inverse pattern was characterized by the acid phosphatase content: (2.748 ± 0.019) u / g in 3 hours and (4.49 ± 0.032) u / g in 13 hours. These markers are the most significant to demonstrate the patterns and can be used as critically important signs in forensic diagnosis.


2014 ◽  
Vol 10 (2) ◽  
Author(s):  
Kyriaki Kitikidou ◽  
Malamati Papakosta ◽  
Dimitris Bakaloudis ◽  
Christos Vlachos

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 539
Author(s):  
Urška Kuhar ◽  
Diana Žele Vengušt ◽  
Urška Jamnikar-Ciglenečki ◽  
Gorazd Vengušt

Papillomaviruses (PVs) are an extremely large group of viruses that cause skin and mucosal infections in humans and various domestic and wild animals. Nevertheless, there is limited knowledge about PVs in wildlife hosts, including mustelid species. This study describes a case in stone marten (Martes foina) with a clinical manifestation of skin tumor, which is rather atypical for infections with PVs. The result of the papillomavirus PCR performed on the skin tumor sample was positive, and the complete PV genome was determined in the studied sample using next-generation sequencing technology. The analysis of the PV genome revealed infection of the stone marten with a putative new PV type belonging to the Dyonupapillomavirus genus. The proposed new stone marten PV type was named MfoiPV1.


2018 ◽  
Vol 16 ◽  
pp. 163-172
Author(s):  
C. Olmedo ◽  
◽  
P. Refoyo ◽  
D. García ◽  
B. Muñoz ◽  
...  

Mammal Study ◽  
2013 ◽  
Vol 38 (4) ◽  
pp. 293-298 ◽  
Author(s):  
Masumi Hisano ◽  
Evgeniy G. Raichev ◽  
Hiroshi Tsunoda ◽  
Ryuichi Masuda ◽  
Yayoi Kaneko

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Dimitrios E. Bakaloudis ◽  
Christos G. Vlachos ◽  
Malamati A. Papakosta ◽  
Vasileios A. Bontzorlos ◽  
Evangelos N. Chatzinikos

Stone martens (Martes foina) are documented as generalist throughout their distributional range whose diet composition is affected by food availability. We tested if this occurs and what feeding strategies it follows in a typical Mediterranean ecosystem in Central Greece by analysing contents from 106 stomachs, seasonally collected from three different habitats during 2003–2006. Seasonal variation in diet and feeding strategies was evident and linked to seasonal nutritional requirements, but possibly imposed by strong interference competition and intraguild predation. Fleshy fruits and arthropods predominated in the diet, but also mammals and birds were frequently consumed. An overall low dietary niche breadth (BA=0.128) indicated a fruit specialization tendency. A generalised diet occurred in spring with high individual specialisation, whereas more animal-type prey was consumed than fruits. A population specialization towards fruits was indicated during summer and autumn, whereas insects were consumed occasionally by males. In those seasons it switched to more clumped food types such as fruits and insects. In winter it selectively exploited both adult and larvae insects and partially fruits overwinter on plants. The tendency to consume particular prey items seasonally reflected both the population specialist behaviour and the individual flexibility preyed on different food resources.


2011 ◽  
Vol 94 (2) ◽  
pp. 446-452 ◽  
Author(s):  
Donglei Yu ◽  
Nathan Rummel ◽  
Badar Shaikh

Abstract An HPLC method was developed for the determination of albendazole (ABZ) and its metabolites, a sulfoxide (ABZSO), a sulfone (ABZSO2), and albendazole-2-aminosulfone (ABZ-2-NH2SO2), from yellow perch muscle tissue with adhering skin. The muscle tissue samples were made alkaline with potassium carbonate and extracted with ethyl acetate, followed by a series of liquidliquid extraction steps. After solvent evaporation, the residue was reconstituted in the initial mobile phase combination of the gradient. The mobile phase consisted of a buffer, 50 mM ammonium acetate (pH 4.0) in 10 methanolwater, and 100 acetonitrile. The gradient was from 20 acetonitrile to 85 acetonitrile. The analytes were chromatographed on an RP Luna C18(2) column and detected by fluorescence with excitation and emission wavelengths of 290 and 330 nm, respectively. The average recoveries from fortified muscle tissue for ABZ (20100 ppb), ABZ-SO (20200 ppb), ABZSO2 (8100 ppb), and ABZ-2-NH2SO2 (20100 ppb) were 85, 95, 101, and 86, respectively, with corresponding CV values of 9, 3, 6, and 4, respectively. Their LOQ values were 10, 10, 1, and 10 ppb, respectively. The procedure was applied to determine ABZ and its major metabolites in the incurred muscle tissue of yellow perch obtained after orally dosing the fish with ABZ.


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