INTRODUCTION : Anxiety as a disorder is increasing worldwide due to complexity of daily life in modern society. In India it is reported to be
highest among all psychiatry disorders, thus highlighting the importance of treating anxiety disorders. Benzodiazepines are widely prescribed
drugs for treatment but their clinical uses are limited due to side effects. Citrus Limon is a highly reputed plant and has been widely employed in
complementary and alternative medicine but no signicant work has been carried out on the anxiolytic effects of this plant extract.The present
study was designed to experimentally evaluate the anxiolytic effects of ethanolic extract of leaves of Citrus Limon on wistar rats using three
different doses orally i.e 50,100,200 mg/kg on open eld test..
MATERIAL& METHODS: Atotal of 30 wistar rats of either sex were divided into 5 groups with 6 rats each. Group I(control): received distilled
water. Group II(standard):Diazepam 2mg/kg is given. Group III -50mg/kg of extract is given. Group IV-100mg/kg is given. Group V- 200mg/kg is
given. Data analyzed by one way ANOVA and Post hoc comparisions were performed by Tukey Kramer multiple comparision test.P< 0.05 was
considered signicant.
RESULTS : Ethanolic extract of Citrus Limon in dose of 100mg/kg showed effective anxiolytic activity compared to 50,200 mg/kg dose and
effect is compared to Diazepam.
CONCLUSION:The present study suggest that Citrus Limonmay be developed as a potential anti anxiety agent.
Preclinical evaluation of anxiolytic activity of Enhydra Flactuans leaves on albino Wistar rats
