scholarly journals EFFECT OF CELL PROTECTANTS ON MASS MULTIPLICATION OF AZOTOBACTER

Keyword(s):  
2020 ◽  
Vol 5 (01) ◽  
pp. 138-144
Author(s):  
Ankush Taliyan ◽  
Ajay Kumar ◽  
Rajendra Singh ◽  
Rohit Rana ◽  
Shekhar Rana

Entomopathogenic fungi (EPF) Metarhizium anisopliae are fungal species that is the frequently occurring and destructive pest management to the pathogenic soil and insects. The effect of different substrates for the mass production of Metarhizium anisopliae spore/ml was significantly higher recorded The results revealed that all the treatments were significantly producing spore per ml and thus increasing the yield significantly as compared to other substrates. The results revealed that all the treatments were significantly higher effective in producing spore/ml as compared to other substrates overall finding showed that substrate tested, for Metarhizium anisopliae spore/ml production was significantly higher recorded 240.53 and 195.26 spore/ml were recorded on substrate irrespective of the temperature.


2010 ◽  
Vol 32 (3) ◽  
pp. 445-449 ◽  
Author(s):  
Aline Vieira Santos ◽  
Maria Fátima Arrigoni-Blank ◽  
Arie Fitzgerald Blank ◽  
Fernanda Ferreira Tavares ◽  
Roberta Pereira Miranda Fernandes ◽  
...  

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Shastri P. Shukla ◽  
P. B. Khare

This study was undertaken to identify methods of mass multiplication for five ornamental, economically important ferns (Nephrolepis biserrata (Sw.) Schott., N. cordifolia cv. ‘‘duffii’’ (L.) Presl., N. exaltata cv. bostoniensis (L.) Schott., Pteris vittata L., and Cyclosorus dentatus Link.,) and three threatened ferns, namely, Cyathea spinulosa Wall. ex. Hook, Pityrogramma calomelanos (L.) Link., and Microsorum punctatum (L.) Schott., through in vitro techniques. Collections were made from different biodiversity zones of India including Northeast Himalayas, Kumaon Himalayas, and Western Ghat and successfully introduced and grown in a fern-house. Aseptic cultures were raised at the morphogenic level of callus, axillary shoot, multiple shoot, and rooted plants. An optimized medium is described for each fern species. Plantlets were also produced from spore culture of Cyathea spinulosa and successfully hardened under fern house conditions.


2016 ◽  
Vol 25 (2) ◽  
pp. 181-191
Author(s):  
Bakul Bhattacharjee ◽  
SM Shahinul Islam ◽  
Sreeramanan Subramaniam

A rapid and an efficient protocol for germination of immature seeds of Vanda tessellata, PLBs development and plant regeneration system have been developed. The results indicate that immature capsules performed better in terms of germination (100.0%) and development of PLBs (90.0%). Around 45 - 60 days old PLBs were transferred to half strength of MS supplemented with different concentrations of auxins and cytokinins. The highest weight of PLBs (5.53 g), the best plant regeneration (16.50) and shoot length (3.25 cm) were recorded when the medium was supplemented with BAP (1.0 mg/l) and NAA (1.5 mg/l). The highest number (5.0) and length (3.22 cm) of roots, the maximum number of plantlets (3.75) were obtained in the medium supplemented with 1.5 mg/l BAP and 1.0 mg/l IAA. The maximum numbers of leaves (3.33) were recorded in the medium fortified with 1.5 mg/l BAP + 1.00 mg/l IAA; the highest length of leaves (2.59 cm) was observed in the medium combining with of BAP and IAA (2.0 mg/l). Well shooted - and rooted plants were acclimated in pots that contained two types of compost. Here, type-II showed better performance on plant development.Plant Tissue Cult. & Biotech. 25(2): 181-191, 2015 (December)


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