Mass multiplication of Metarhizium anisopliae on different media

2020 ◽  
Vol 5 (01) ◽  
pp. 138-144
Author(s):  
Ankush Taliyan ◽  
Ajay Kumar ◽  
Rajendra Singh ◽  
Rohit Rana ◽  
Shekhar Rana
Keyword(s):  
Pest Management ◽  
Entomopathogenic Fungi ◽  
Mass Production ◽  
Metarhizium Anisopliae ◽  
Fungal Species ◽  
Mass Multiplication

Entomopathogenic fungi (EPF) Metarhizium anisopliae are fungal species that is the frequently occurring and destructive pest management to the pathogenic soil and insects. The effect of different substrates for the mass production of Metarhizium anisopliae spore/ml was significantly higher recorded The results revealed that all the treatments were significantly producing spore per ml and thus increasing the yield significantly as compared to other substrates. The results revealed that all the treatments were significantly higher effective in producing spore/ml as compared to other substrates overall finding showed that substrate tested, for Metarhizium anisopliae spore/ml production was significantly higher recorded 240.53 and 195.26 spore/ml were recorded on substrate irrespective of the temperature.

scholarly journals Biological insect control using Metarhizium anisopliae: morphological, molecular, and ecological aspects

2014 ◽  
Vol 44 (4) ◽  
pp. 645-651 ◽  
Author(s):  
Patricia Vieira Tiago ◽  
Neiva Tinti de Oliveira ◽  
Elza Áurea de Luna Alves Lima
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Microbial Control ◽  
Intraspecific Variability ◽  
Fungal Species ◽  
Insect Control ◽  
Pest Population ◽  
Ecological Aspects ◽  
Population Structures ◽  
Biological Insect Control

Microbial control of insects is based on the rational use of pathogens to maintain environmentally balanced pest population levels, and Metarhizium anisopliae has been the most studied and most utilized fungal species for that purpose. The natural genetic variability of entomopathogenic fungi is considered one of the principal advantages of microbial insect control. The inter- and intraspecific variability and the genetic diversity and population structures of Metarhizium and other entomopathogenic fungi have been examined using ITS-RFLP, ISSR, and ISSP molecular markers. The persistence of M. anisopliae in the soil and its possible effects on the structures of resident microbial communities must be considered when selecting isolates for biological insect control.

scholarly journals Application of Bait Treated with the Entomopathogenic FungusMetarhizium anisopliae(Metsch.) Sorokin for the Control ofMicrocerotermes diversusSilv.

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Amir Cheraghi ◽  
Behzad Habibpour ◽  
Mohammad Saied Mossadegh
Keyword(s):  
Biological Control ◽  
Pest Management ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Fungal Pathogen ◽  
Management Strategy ◽  
Khuzestan Province ◽  
Significant Difference ◽  
Strategy Performance ◽  
Bait System

Microcerotermes diversusSilvestri (Isoptera, Termitidae) is considered to be the most destructive termite in Khuzestan province (Iran), and its control by conventional methods is often difficult. Biological control using entomopathogenic fungi could be an alternative management strategy. Performance of a bait matrix treated with the entomopathogenic fungusMetarhizium anisopliae(Metsch.) Sorokin, Strain Saravan (DEMI 001), againstM. diversuswas evaluated in this paper. The highest rate of mortality occurred at concentrations of 3.7 × 107and 3.5 × 108(conidia per mL). There was no significant difference between treatments, in the rate of feeding on the bait. The fungal pathogen was not repellent to the target termite over the conidial concentrations used. The current results suggest potential of such bait system in controlling termite. However the effectiveness ofM. anisopliaeas a component of integrated pest management forM. diversusstill needs to be proven under field conditions.

scholarly journals Microbial control of white grubs with entomopathogenic fungi and its value in integrated pest management (IPM) in Nepal

1970 ◽  
Vol 24 (1) ◽  
pp. 31-36
Author(s):  
Yubak Dhoj G. C. ◽  
Siegried Keller ◽  
Peter Nagel
Keyword(s):  
Pest Management ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Fungal Pathogens ◽  
Microbial Control ◽  
White Grubs ◽  
First Results ◽  
Crop Disease ◽  
Future Work

With an objective to explore the possibility of biocontrol of white grubs using entomopathogenic fungi, an exploratory tudy was conducted in the Syangja and Parbat districts in nepal in the winter of 2001/2002. In order to explore the occurrence of indigenous fungal pathogens of white grubs, field and laboratory experiments were carried out and information were collected from all available sources. Upon collection of the white grubs the entomopathogenic fungus Metarhizium anisopliae was found to be associated with white grubs in fields with arable crop. Disease prevalence was between ) and 2% depending on host origin and species. Bioassays revealed that the Nepalese isolates of this fungus species were as pathogenic as a Swiss isolate used for comparison purposes. Therefore, Future work will be done exclusively with Nepalese isolates. Analysis of soils from three different regions showed that M. anisopliae is common and was present in about 50% of the samples irrespective of their origin. However, the fungus densities were low. Another entomopathogenic fungus, Beauveria bassiana, was isolated as well from a few soil samples. Based on these first results, the possibilities to develop mycoinsecticides and to integrate them into existing pest management (IMP) systems are considered as very promising. In the meantime a project funded by Helvetas has been initiated at Tribhuvan University (TU), Institute of Agriculture and Animal Sciences (IAAS), Rampur, Chitwan, Nepal. Key words: Biological control, entomopathogenic fungi, Metarhizium anisopliae, Scarabaeidae, white grubs, Nepal.

scholarly journals Mass Production of Entomopathogenic Fungi (Metarhizium anisopliae) using Different Grains as a Substrate

2018 ◽  
Vol 7 (1) ◽  
pp. 2227-2232 ◽  
Author(s):  
S.V. Agale ◽  
S. Gopalakrishnan ◽  
K.G. Ambhure ◽  
Hemkant Chandravanshi ◽  
Rajeev Gupta ◽  
...  
Keyword(s):  
Entomopathogenic Fungi ◽  
Mass Production ◽  
Metarhizium Anisopliae

scholarly journals Pathogenicity of indigenous strains of three entomopathogenic fungi to the sisal weevil, Scyphophorus acupunctatus (Gyllenhal) (Coleoptera: Curculionidae)

2015 ◽  
Vol 8 (2) ◽  
pp. 46-54
Author(s):  
V.T. Gkounti ◽  
D. Markoyiannaki ◽  
D.Ch. Kontodimas
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Fungal Species ◽  
Life Stages ◽  
Biological Control Agents ◽  
Isaria Fumosorosea ◽  
Mean Survival Time ◽  
High Pathogenicity

SummaryThe pathogenicity of indigenous isolates of Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea was evaluated in the laboratory against larvae and adults of the sisal weevil, Scyphophorus acupunctatus. Inoculation was achieved via immersion of individuals into conidia suspensions of different concentrations. All three fungal species proved high pathogenicity against larvae of the weevil, causing 100% mortality in most of the treatments. Beauveria bassiana caused the highest mortality of the adults (86.67±12%), followed by M. anisopliae (46.67±17.8%) and I. fumosorosea (40±17.5%). Mean survival time also differed significantly among treatments and life stages of the weevil. In total, larvae survived significantly fewer days than adults post infection. Results of the present study indicate the potential of indigenous strains of entomopathogenic fungi as biological control agents against the invasive weevil.

Evaluation of entomopathogenic fungi by larval and adult immersion method v1

2021 ◽  
Author(s):  
Lissette Torres-Torres ◽  
Carlos Espinel-Correal ◽  
Adriana Santos-Díaz
Keyword(s):  
Biological Control ◽  
Pest Management ◽  
Entomopathogenic Fungi ◽  
Biological Control Agent ◽  
Control Agent ◽  
Fungal Species ◽  
Immersion Method ◽  
Selection Of

The search for commercially viable entomopathogenic fungi for use in integrated pest management programs involves several steps. Fungal species must first be obtained from diseased insects or the environment and identified. Then, they must be evaluated under laboratory conditions to identify the most promising candidates. Because of that, bioassays must be repeatable and reliable to determine accurate pathogenicity or virulence. Variability in results may be caused by the variation in the components of an assay. However, the availability of a standardized bioassay is limited. Few reports detail the methods used to develop bioassays for a specific purpose and, without these details, it is difficult to develop bioassay methodologies suitable to evaluate the fungus-host relationship. We described a protocol based on the immersion method to evaluate entomopathogenic fungi (larval and adult stages), that can be reproduced to reduce variability. This protocol can be used in several stages of biopesticide development: selection of the biological control agent, characterization of the microorganism, formulation compatibility, and in vitro evaluation of efficacy.

scholarly journals On the virulence of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Ascomycota: Hypocreales), against the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Shehzad ◽  
Muhammad Tariq ◽  
Tariq Mukhtar ◽  
Asim Gulzar
Keyword(s):  
Sex Ratio ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plutella Xylostella ◽  
Metarhizium Anisopliae ◽  
Insect Pest ◽  
Diamondback Moth ◽  
Economic Damage ◽  
Offspring Sex Ratio ◽  
Development Of Resistance

Abstract Background The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a noxious pest of cruciferous crops all over the world causing serious economic damage. Management of insect pest generally depends on chemical control; however, due to development of resistance against all types of insecticides, alternative approaches especially utilization of a microbial agent is inevitable. Results Potential of 2 entomopathogenic fungi (EPF), viz., Beauveria bassiana and Metarhizium anisopliae, was evaluated against 2nd and 3rd larval instars of P. xylostella by adopting leaf dip and direct spraying methods under laboratory conditions. Significant mortality rate was achieved by each fungus under adopted methodologies. However, B. bassiana was found to be more effective in both conditions than M. anisopliae. Highest mean corrected mortality (77.80%) was recorded, when spores of B. bassiana were sprayed on the 2nd instar larvae (LC50=1.78×104/ml) after the 6th day of treatment. Similarly, incase of M. anisopliae LC50 for the 2nd instar at the same methodology was 2.78×104/ml with a mortality percentage of 70.0%. Offspring sex ratio was non-significantly related to treatment concentration and methodology, except for the control. Conclusion Beauveria bassiana and M. anisopliae had potential to suppress P. xylostella infestations when applied appropriately. Present findings suggested that B. bassiana and M. anisopliae when sprayed on immatures of host insect had more effect as compared to leaf dip procedure. Furthermore, no significant effect of concentrations was observed on sex ratio.

scholarly journals Growth of entomopathogenic fungi colonies Metarhizium anisopliae (Metchnikoff) Sorokin enriched with termite juice

2021 ◽  
Vol 667 (1) ◽  
pp. 012084
Author(s):  
N Anggraini ◽  
S Suhartono ◽  
A Alfizar ◽  
H Husni ◽  
S Rusdiana ◽  
...  
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae

scholarly journals Entomopathogenic fungi as the microbial frontline against the alien Eucalyptus pest Gonipterus platensis in Brazil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Carolina Jordan ◽  
Paula Leite dos Santos ◽  
Leiliane Rodrigues dos Santos Oliveira ◽  
Mauricio Magalhães Domingues ◽  
Bianca Cristina Costa Gêa ◽  
...  
Keyword(s):  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Natural Variation ◽  
Fungal Strains ◽  
Conidial Production ◽  
Laboratory Conditions ◽  
Virulent Strains ◽  
Eucalyptus Plantations ◽  
Infected Adult

AbstractThe eucalyptus snout beetle (ESB), Gonipterus platensis, is endemic to Australia but has become a major invasive, destructive pest of Brazilian eucalyptus plantations. Efforts to develop insecticides based on entomopathogenic fungi against ESB are limited by the lack of known virulent strains. We therefore explored the virulence of indigenous Brazilian strains of major entomopathogenic fungi—Beauveria spp. and Metarhizium anisopliae—against ESB adults. We found widely varying virulence and later capacities for conidial production on infected adult cadavers. Two strains stood out, B. bassiana IBCB-240 and M. anisopliae IBCB-364, as especially lethal for ESB adults under laboratory conditions, sporulated abundantly on infected insects, and also outperformed comparable strains used in commercial mycoinsecticides. Notably, B. bassiana IBCB-240 exhibited lower LT50 values at low inoculum levels (≤ 107 conidia mL−1) and smaller LC50 values than M. anisopliae IBCB-364. Taken together, this study emphasizes natural variation in virulence among indigenous Beauveria and Metarhizium strains against ESB adults and identifies fungal strains with superior lethality to existing commercialized strains for managing this eucalyptus pest in Brazil.

Sign in / Sign up

Export Citation Format

Share Document