scholarly journals Detection of H. pylori in Pediatric Patients’ Stool Sample by Multiplex Urease PCR

2022 ◽  
Vol 13 (1) ◽  
Author(s):  
Sinem ÖKTEM OKULLU ◽  
Oğuz Can IŞIK ◽  
Sesın KOCAGÖZ ◽  
Işın AKYAR
Keyword(s):  
Stool Sample ◽  
Pediatric Patients ◽  
H Pylori

scholarly journals Antibiotic Resistance of Helicobacter pylori Strains Isolated From Pediatric Patients in Southwest China

2021 ◽  
Vol 11 ◽  
Author(s):  
Juan Li ◽  
Jianjun Deng ◽  
Zhiling Wang ◽  
Hong Li ◽  
Chaomin Wan
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Pediatric Patients ◽  
Southwest China ◽  
Pediatric Population ◽  
Primary Resistance ◽  
Secondary Resistance ◽  
Treatment Naïve ◽  
H Pylori ◽  
Resistance Rates

The number of antibiotics that are appropriate for Helicobacter pylori eradication in children is limited. Profiling regional or population-specific antibiotic resistance is essential in guiding the H. pylori eradication treatment in children. The aim of this study was to evaluate the antibiotic resistance in H. pylori strains isolated from children and adolescents in Southwest China. Gastric biopsies from 157 pediatric patients with or without previous H. pylori eradication treatment were collected for H. pylori culture. Susceptibility to amoxicillin (AML), clarithromycin (CLR), metronidazole (MTZ), levofloxacin (LEV), tetracycline (TET), furazolidone (FZD), and rifampicin (RIF) was determined by E-test or a disk diffusion assay. A total of 87 patients from three ethnic groups (Han/Tibetan/Yi) were H. pylori culture positive (55.4%). The overall resistance rates were 55.2% for CLR, 71.3% for MTZ, 60.9% for RIF, and 18.4% for LEV. No isolate was found to be resistant to AML, TET, and FZD. Among the 53 treatment-naïve pediatric patients, primary resistance rates to clarithromycin, metronidazole, levofloxacin, and rifampicin were 45.3, 73.6, 15.1, and 60.4%, respectively. Among the 34 treatment-experienced patients, secondary resistance rates to clarithromycin, metronidazole, levofloxacin, and rifampicin were 70.6, 67.6, 23.5, and 61.8%, respectively. Isolates exhibiting simultaneous resistance to clarithromycin and metronidazole were 28.3 and 52.9% among the treatment-naïve and treatment-experienced patients, respectively. In conclusion, among pediatric patients in Southwest China, resistance rates were high for clarithromycin, metronidazole, levofloxacin, and rifampicin, whereas nil resistance was found to amoxicillin, tetracycline, and furazolidone. Our data suggest that the standard clarithromycin-based triple therapy should be abandoned as empiric therapy, whereas the bismuth quadruple therapy (bismuth/PPI/amoxicillin/tetracycline) would be suitable as first-line empiric treatment regimen for this pediatric population. Tetracycline and furazolidone may be considered for treating refractory H. pylori infections in adolescent patients.

scholarly journals Efficacy of Helicobacter pylori Eradication Therapy on Platelet Recovery in Pediatric Immune Thrombocytopenic Purpura-Case Series and a Systematic Review

2020 ◽  
Vol 8 (10) ◽  
pp. 1457
Author(s):  
Tamaki Ikuse ◽  
Masanori Toda ◽  
Kosuke Kashiwagi ◽  
Kimiko Maruyama ◽  
Masumi Nagata ◽  
...  
Keyword(s):  
Systematic Review ◽  
Helicobacter Pylori ◽  
Immune Thrombocytopenic Purpura ◽  
Pediatric Patients ◽  
Eradication Therapy ◽  
Platelet Response ◽  
Thrombocytopenic Purpura ◽  
Helicobacter Pylori Eradication ◽  
H Pylori ◽  
Helicobacter Pylori Eradication Therapy

Evidence relating to the efficacy of Helicobacter pylori eradication therapy for chronic immune thrombocytopenic purpura (cITP) in childhood is inadequate. The aim of this retrospective study was to determine the efficacy of H. pylori eradication therapy for platelet response in pediatric patients with cITP in our hospital, and to perform a systematic review of previous reports about pediatric patients with cITP who were positive for H. pylori infection and were treated with H. pylori eradication therapy. Analysis of the data of pediatric patients with cITP in our hospital and a systematic review of digital literature databases of studies in pediatric patients with cITP were performed. Data of 33 pediatric patients with cITP from our hospital records showed that the prevalence of H. pylori infection and the rate of response to platelet therapy were 15% and 33.3%, respectively. Data of 706 pediatric patients from 18 previous reports showed that the prevalence of H. pylori infection and rate of platelet response were 23% and 43.8%, respectively. Eradication therapy for H. pylori infection in pediatric cITP patients can be expected to result in a response equivalent to that in the adult population, with fewer adverse effects than other treatments for cITP.

scholarly journals Two Enzyme Immunoassays and PCR for Detection ofHelicobacter pylori in Stool Specimens from Pediatric Patients before and after Eradication Therapy

2000 ◽  
Vol 38 (10) ◽  
pp. 3710-3714 ◽  
Author(s):  
Athanasios Makristathis ◽  
Wolfgang Barousch ◽  
Eva Pasching ◽  
Christa Binder ◽  
Christa Kuderna ◽  
...  
Keyword(s):  
Pediatric Patients ◽  
Eradication Therapy ◽  
Test Series ◽  
Diagnostic Tools ◽  
The Novel ◽  
Enzyme Immunoassays ◽  
End Of Treatment ◽  
Before And After ◽  
Stool Samples ◽  
H Pylori

This study of pediatric patients was intended to determine the suitability of stool PCR and two antigen enzyme immunoassays (EIAs; Premier Platinum HpSA and the novel FemtoLab H. pylori), which detectHelicobacter pylori antigens in feces, as pretreatment diagnostic tools and especially as posttreatment control. Forty-nineH. pylori-infected children with dyspepsia received eradication therapy. Successful treatment was determined by a negative [13C]urea breath test 4 and 12 weeks after discontinuation of therapy. Fecal specimens were collected prior to eradication therapy as well as 4 weeks after the end of treatment. Successfully treated children delivered stool samples at 6, 8, and 12 weeks posttreatment also. Specimens were examined by seminested PCR and Premier Platinum HpSA and were reexamined by both EIAs as soon as FemtoLab H. pylori was available. In the first test series, the overall sensitivities of PCR and Premier Platinum HpSA were 93.0 and 91.1%, respectively. With specimens collected at 4 weeks after treatment, the respective specificities were 68.8 and 79.3%. After longer follow-up periods, however, they gradually increased to 100 and 96.9%, respectively. In the new test series, Premier Platinum HpSA delivered a considerably lower number of false-positive results (4 versus 18), indicating intertest variations. The overall test sensitivity was 94.6%, and the overall specificity was 97.5%. FemtoLab H. pylori showed an excellent performance with an overall sensitivity and specificity of 98.2 and 98.1%, respectively. Thus, in contrast to PCR, both EIAs were shown to be suitable for early posttreatment control.

Evaluation of serological assays for H pylori, CagA and VacA antibodies in pediatric patients with abdominal pain

1998 ◽  
Vol 114 ◽  
pp. A92-A93
Author(s):  
SKF Chong ◽  
TV Nowak ◽  
JA Nowak ◽  
S Mohindra ◽  
N Iglitzen ◽  
...  
Keyword(s):  
Abdominal Pain ◽  
Pediatric Patients ◽  
H Pylori

scholarly journals Antimicrobial susceptibility of H. pylori isolated from suspected patient using stool sample at Malali Hospital and Maternity Clinic Kaduna, Kaduna State – Nigeria

2021 ◽  
Vol 14 (3) ◽  
pp. 122-128
Author(s):  
Rabilu Sani
Keyword(s):  
Helicobacter Pylori ◽  
Stool Sample ◽  
Antimicrobial Susceptibility ◽  
Atmospheric Oxygen ◽  
Bacterial Cells ◽  
Selective Media ◽  
H Pylori ◽  
Phosphate Buffered Saline ◽  
Maternity Clinic ◽  
Environment Condition

The antimicrobial susceptibility of Helicobacter pylori isolated from suspected patient using stool sample at Beijing greatest college of health sciences and technology, Kaduna, demonstration clinic. The analyses were carried out to further understand the intestinal environment condition of the sample sites. As it has been noted that: it is difficult of growing Helicobacter pylori in a broth, hence, no specific enrichment has been proposed. Note: As everything present in the stomach would be found in the stools, hence there is no doubt that Helicobacter pylori can be eliminated via this route and successful culturing of Helicobacter pylori from the stool sample had been achieved. [4]. The stool was diluted to a 20% w/v solution of in phosphate buffered saline (PBS) and the suspension was sieved through a 250 um strainer before plating onto the selective media. The sample was passed through a series of dilution techniques and centrifugation before finally plating on to the deoxycholate citrate agar (selective) and incubates at 37oC under the microacrobic atmospheric oxygen concentration of 7 – 12% (O2) for four (4) days. Across the cultured, the cultured plate there was marked abundant of bacterial cells with some colonies having the characteristics of Helicobacter pylori which later sub-cultured and isolated for antimicrobial test.

EVALUATION OF SEROLOGICAL ASSAYS FOR H PYLORI, CagA AND VacA ANTIBODIES IN PEDIATRIC PATIENTS WITH ABDOMINAL PAIN.

1998 ◽  
Vol 26 (5) ◽  
pp. 539
Author(s):  
S. K.F. Chong ◽  
T. V. Nowak ◽  
J. A. Nowak ◽  
S. Mohindra ◽  
N. Iglitzen ◽  
...  
Keyword(s):  
Abdominal Pain ◽  
Pediatric Patients ◽  
H Pylori

scholarly journals Helicobacter pylori Infection: Clinical, Endoscopic, and Histological Findings in Lebanese Pediatric Patients

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
F. AL Kirdy ◽  
M. Rajab ◽  
N. El-Rifai
Keyword(s):  
Helicobacter Pylori ◽  
Gastrointestinal Endoscopy ◽  
Pediatric Patients ◽  
Epigastric Pain ◽  
Demographic Data ◽  
Upper Gastrointestinal Endoscopy ◽  
Histopathological Findings ◽  
Increased Risk ◽  
H Pylori ◽  
Upper Gastrointestinal

Background. Helicobacter pylori (H. pylori) is a common and universally distributed bacterial infection. However, in children, active gastritis and ulcer are rarely seen. Objectives. The aims of this study were to establish the prevalence of H. pylori infection and to compare the clinical, endoscopic, and histopathological findings between infected and noninfected pediatric patients at Makassed General Hospital. Methods. Patients aged between 1 month and 17 years who underwent upper gastrointestinal endoscopy from January 2011 to January 2017 were included. The diagnosis of H. pylori was confirmed by a CLO test and/or its presence on biopsy specimens. Demographic data, clinical characteristics, endoscopic and histopathological findings, and gastritis score were recorded retrospectively. Results. During the study period, 651 children underwent upper gastrointestinal endoscopy. The main indication was abdominal pain (61%). The prevalence of H. pylori infection was 16.5%. The infection was most commonly seen among children aged between 6 and 10 years (43%). A large number of family members were associated with increased risk of infection (4.8±1.5 versus 5.2±1.8; p<0.05). Epigastric pain was more associated with H. pylori (61.3% versus 14.6% in noninfected patients; p<0.05). Nodular gastritis was commonly seen in infected patients (41.5% vs. 7.9%; p<0.05). Mild and moderate gastritis was seen more in infected versus noninfected patients (mild: 53.8% vs. 14%; moderate: 27.4% vs. 2.4%, respectively). Conclusion. Although epigastric pain was associated with H. pylori, other diagnoses should be considered since the infection are rarely symptomatic in children. Antral nodularity was associated with H. pylori infection; however, its absence does not preclude the diagnosis.

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