scholarly journals FEATURES OF THE IMMUNOGENETIC STRUCTURE OF PIGS OF DOMESTIC BREEDS, SUITABLE FOR XENOTRANSPLANTATION

2020 ◽  
Vol 59 ◽  
pp. 105-114
Author(s):  
O. I. Metlytska ◽  
T. N. Ryk ◽  
V. I. Rossokha ◽  
A. A. Saenko
Keyword(s):  
Blood Group ◽  
Molecular Genetic ◽  
Statistical Processing ◽  
Molecular Genetic Analysis ◽  
Blood Groups ◽  
Marker Allele ◽  
Allele Distribution ◽  
Specialized Line ◽  
Selection Of ◽  
Immunogenetic Analysis

The aim of the work was to determine the immunogenetic characteristics of pigs of two Ukrainian breeds by their adaptability, resistance, reproduction, genetic homogeneity, the presence of alleles that determine the potential suitability for use in xenotransplantation and other biomedical purposes. At present, there is no breed or specialized line of pigs in Ukraine to address the urgent problems of humane medicine. However, there is a favorable situation for the creation of such a breed or the withdrawal of a specialized line because of the demand of pharmacological concerns for model biological objects to study the mechanisms of action of modern medical preparations, to develop methods of conducting bloodless surgical operations, modeling of the clinic and epidemiology of infectious diseases of different diseases. immune response, etc. In this context, there was a compelling reason to preserve the indigenous breeds of pigs of Ukraine, especially Ukrainian meat and Мyrgorod. Standard methods of immunogenetic analysis of pigs were used. The erythrocyte antigens of 9 blood group systems were determined using specific immune sera and the involvement of a bank of immunodiagnostics meeting international requirements. Blood groups were determined by the reaction of agglutination, an indirect Coombs test, and a hemolytic test. Statistical processing of the research results was performed using mathematical statistics using the GenAlex-6.0 computer program. As a result of the immunogenetic analysis of Ukrainian beef and myrrh-pig breeds, it was determined that each of them is characterized by a specific immunogenic profile, which is related to both breed characteristics and differences, and methods of their breeding. The difference between the distribution of the overwhelming number of alleles of blood groups was statistically significant. Pigs of Ukrainian meat and Мyrgorod breeds had the largest differences in allele distribution by B, E, F, K, L blood group systems with the presence of the Ladhjk marker allele in the latter (p < 0.05). The specificity of immunogenic profiles was evaluated, depending on the history of creation and the direction of the animal's performance. Species of Myrhorod and Ukrainian meat breeds with the presence of genotypes A - / - and Ebdgkmp / bdgkmp were found to determine the suitability of animals for xenotransplantation. Of the 80 animals tested for immunogenetic markers, only 24 individuals met the established selection criteria. The range of determined indicators of actual homozygosity for selected genotype animals ranged from 55.56% to the maximum value – 88.89%, mainly in sows from the Smorodinа, Rusalkа, Sorokа and Soyka families. According to molecular genetic analysis, you have been informed that the established criteria of the biomedical model meet only 13 individuals of Ukrainian meat breed. Among animals with the highest index of actual homozygosity, representatives of Cererа and Cіlina were identified, with Celina 4092 being homozygous for all blood group systems studied. The selection of pigs for blood alleles, desirable for xenotransplantation, with their transfer to the homozygous state: A -/- and Ebdgkmp / bdgkmp will impair the adaptive, first of all, reproductive qualities of the animals and create significant problems for the cultivation of such individuals. The possibility of the occurrence of reproductive and resistance disorders of pigs in the selection of blood groups genotypes modeled for xenotransplantation and the search for ways to overcome them are being considered.

scholarly journals Molecular Genetic Analysis of the Human Lewis Histo-blood Group System

1996 ◽  
Vol 271 (16) ◽  
pp. 9830-9837 ◽  
Author(s):  
Takashi Kudo ◽  
Hiroko Iwasaki ◽  
Shoko Nishihara ◽  
Naoko Shinya ◽  
Takao Ando ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Blood Group ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Blood Group System ◽  
Group System

scholarly journals Identification of Gene Related to Hard Bunch Phenotype in Oil Palm (Elaeis guineensis Jacq.)

2015 ◽  
Vol 43 (2) ◽  
pp. 147
Author(s):  
Roberdi , ◽  
Sobir , ◽  
Sudirman Yahya ◽  
Nurita Toruan-Mathius ◽  
Tony Liwang
Keyword(s):  
Dna Sequences ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Target Sequence ◽  
Polymorphic Markers ◽  
Copia Retrotransposon ◽  
Selection Of

<p>ABSTRACT</p><p>Molecular genetic analysis of hard bunch phenomenon in oil palm was done in order to elucidate the role of genetic factor underlying hard bunch in oil palm plantation. The aim of this study was to identify the AFLP primer combination that co-segregates with hard bunch phenotype related gene in oil palm. Molecular analysis was done by bulk segregant analysis approach. DNA was isolated from leaves of the normal and hard bunch palm. DNA from ten individual palms from each category were pooled and used as a template. A total of 56 AFLP primer combinations were selected for selection of polymorphic primer, and as a result it was found that 22 AFLP primer combinations (39.28%) were polymorphic. A total of 48 individual of palm DNA containing 24 individual for each group were further genotyped by those 22 polymorphic markers. Of these, one AFLP primer combination (E-ACC/M-CTG) was obtained as a co-segregated marker that distinguished the hard bunch DNA from the normal one. Based on the analysis of the target sequence aligned to the oil palm DNA sequences available in database, we found that our sequence has similarity with Ty-1 copia retrotransposon. This sequence distribute in all 16 linkage group of oil palm genome.</p><p>Keywords: abnormal fruits, AFLP, oil palm, Ty-1 copia retrotransposon</p>

scholarly journals MONITORING OF Aegilops L. LOCAL SPECIES GENETIC DIVERSITY OF KAZAKHSTAN’S FLORA

2018 ◽  
Vol 22 (4) ◽  
pp. 484-490 ◽  
Author(s):  
R. Urazaliev ◽  
M. Yessimbekova ◽  
K. Mukin ◽  
A. Chirkin ◽  
G. Ismagulova
Keyword(s):  
Genetic Diversity ◽  
Global Climate ◽  
Molecular Genetic ◽  
Crop Wild Relatives ◽  
Molecular Genetic Analysis ◽  
Ex Situ ◽  
Marker Allele ◽  
New Genes ◽  
Local Species ◽  
Allelic Variations

Cereal Crop Wild Relatives (CWR) are a very  important gene pool for cereal/wheat improvement. New genes for resistance to diseases and pests are urgently needed to avoid using pesticides and to raise adaptivity to the environmental stresses caused by global climate change. In this regard, the study is aimed at ex situ conservation of Aegilops L. genus local ecotypes’ genetic diversity, which is very relevant and promising for breeding. In order to establish breeding utility and form an ex situ collection reflecting the intra- and inter-specific diversity, the phenotypic screening of Kazakhstan’s local populations of Aegilops L. genus (Ae. cylindrica, Ae. tauschii, Ae. triuncialis and Ae. crassa) was conducted on the basis of multiple indicators. For the first time molecular-genetic analysis of 50 representatives of Aegilops L. genus from Kazakhstan’s flora was performed. The microsatellite analysis with the use of 11 EST-SSR markers revealed eight of them to be most effective. For each marker, allele frequency and average heterozygosity was calculated. For the most informative markers the presence of 5 and 6 respective allelic variations was found. A bank of genomic DNA was created and kept in ex situ storage (–70 °С, long-term) in the IMBB of the MES of RK.

scholarly journals Molecular genetic analysis of some North African barley germplasms

2017 ◽  
Vol 109 (2) ◽  
pp. 187
Author(s):  
Reda Gaafar ◽  
Mai Allam ◽  
Rasha Sabry ◽  
Mahmoud Saker
Keyword(s):  
Rapd Markers ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
North African ◽  
Rt Pcr ◽  
Breeding Selection ◽  
High Level ◽  
Barley Genotypes ◽  
Selection Of ◽  
Selection Of Resistance

<p>Isozyme and RAPD markers were used to characterize 29 barley accessions, which were collected from North Africa. In addition, resistance gene sequences were employed to develop molecular markers using RT-PCR approach. High level of polymorphism was found with both RAPD and isozyme markers, where RAPD showed that 60 % of amplified bands were polymorphic. Peroxidase showed three polymorphic loci (7 allelic bands). Isozymes cluster analysis successfully separated the barley accessions into three geographically distinct groups. RAPD investigation demonstrated that Egyptian accessions were grouped into two obvious groups. Moreover, the Tunisian accessions showed no distinct clustering, while high dissimilarities were revealed by the Algerian accessions. In the RT-PCR, from six primer pairs selected, primer pair AF092524P1P2 successfully amplified two specific amplicons of approximately (340 &amp; 220 bp) and (360 &amp; 270 bp), respectively in two Egyptian barley genotypes (El-Awamah and Awlad-Ali). One primer pair DN988165P1P2 gave only one specific amplicon in both barley genotypes of 250 and 270 bp, respectively. The markers developed could be used in improving barley crop by assisting in breeding selection of resistance genotypes.</p>

scholarly journals Molecular genetic analysis of the Kidd blood group system in a Taiwanese family with Jknull phenotype

2017 ◽  
Vol 1 (4) ◽  
pp. 29-35
Author(s):  
Fang-Yeh Chu ◽  
◽  
Lung-Chih Yu ◽  
Chih-Chun Chang ◽  
Marie Lin
Keyword(s):  
Genetic Analysis ◽  
Blood Group ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Blood Group System ◽  
Group System

Molecular genetic analysis of weak B blood group allele in Jinan population reveals distribution of ABw phenotype

2021 ◽  
Vol 5 (1) ◽  
pp. 53-56
Author(s):  
Wu Wen ◽  
Li Xiaojian ◽  
Guo Xingying ◽  
Wu Peng ◽  
Huang Xiangyan
Keyword(s):  
Genetic Analysis ◽  
Blood Group ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Group Allele ◽  
B Blood Group

INFLUENCE OF PARENT SELECTION METHODS BY B ANTIGEN SIMILARITY INDEX IN THE BLOOD GROUPS ON WEIGHT AND LINEAR GROWTH OF BULLS

2021 ◽  
Vol 12 (4) ◽  
Author(s):  
A. M. Ugnivenko ◽  
O. V. Natalych
Keyword(s):  
Blood Group ◽  
Linear Growth ◽  
Average Daily Gain ◽  
Similarity Index ◽  
Blood Groups ◽  
Selection Methods ◽  
Beef Breed ◽  
Antigenic Similarity ◽  
Parent Selection ◽  
Selection Of

In solving the problem of qualitative improvement of beef breeds, it is important to improve the methods of practical use of existing parent couples selection methods, using histocompatibility antigens, polymorphic proteins and blood group systems. The purpose of the thesis is to determine the influence of homogeneous and heterogeneous selection of parent couples by blood group factors on the weight and linear growth of bulls of Ukrainian beef breed. The Ukrainian beef breed was bred using four breeds and is characterized by high variability in polymorphic features. The type of parent selection was determined by the index of B antigen similarity (ras) of cattle blood groups. The formula of D.A. Zhyvotovskyi and A.M. Mashurov was used to calculate the index of antigenic similarity of parents. Selection by ras of parents ≥ 0,268 was considered homogeneous, and by ras ≤ 0,267 heterogeneous. It has been proven that bulls that are descended from their parents with more ras pravail in the test on average daily gains and have a higher live weight. If ras of the parents is over 0.268, animals tend to improve their growth rate up to 8 months of age. After weaning this trend persists. The average daily gain of bulls obtained from parents with ras up to 0.267 is better in the period from 15 to 18 months, which indicates their lower precocity. If the antigenic similarity index of parents is more than 0.268, the animals are better in terms of the severity of meat forms at the age of 15 and 18 months. At the age of 15 months, bulls obtained from homogeneous selection by ras have smaller height measurements, better developed front of the torso in width and depth of the chest, longer torso and buttocks. Homogeneous selection of parental couples according to the B antigen similarity index of blood groups leads to improvement of weight growth and severity of meat forms of bulls of Ukrainian beef breed.

Molecular genetic analysis of ABO blood group variations reveals 29 novel ABO subgroup alleles

Transfusion ◽  
2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
Xiaohong Cai ◽  
Sha Jin ◽  
Xi Liu ◽  
Liangfeng Fan ◽  
Qiong Lu ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Blood Group ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Abo Blood Group

scholarly journals Molecular genetic analysis of variant phenotypes of the ABO blood group system

Blood ◽  
1996 ◽  
Vol 88 (7) ◽  
pp. 2732-2737 ◽  
Author(s):  
K Ogasawara ◽  
R Yabe ◽  
M Uchikawa ◽  
N Saitou ◽  
M Bannai ◽  
...  
Keyword(s):  
Blood Group ◽  
Molecular Genetic ◽  
Nonsynonymous Substitution ◽  
Single Strand Conformation Polymorphism ◽  
Molecular Genetic Analysis ◽  
Nucleotide Sequences ◽  
Activity Level ◽  
Blood Group System ◽  
Nucleotide Substitutions ◽  
Group System

ABO is clinically the most important blood group system in transfusion medicine and includes many variant phenotypes. To understand the molecular genetic basis of this polymorphic system, we have analyzed genomic DNAs obtained from Japanese individuals possessing variant ABO phenotypes including A2, Ax, Ael, cis-AB, Bx, and Bel. By polymerase chain reaction-single-strand conformation polymorphism (SSCP) and nucleotide sequence analyses, we identified 11 different alleles. These alleles had nucleotide sequences different from those of the previously described 13 different alleles responsible for the common ABO phenotypes. Analysis of the nucleotide sequences of the alleles responsible for those variant phenotypes showed that the amino acid residues at position 266 and 268 may be crucial for transferase specificity, whereas those at positions 214, 216, 223, 291, and 352 may be critical for the activity level. Nine of the 11 alleles, responsible for the A2, Ax, Ael, cis-AB, Bx, and Bel phenotypes, were presumed to be generated from common ABO alleles by single nucleotide mutations such as nonsynonymous substitution, deletion, or insertion. Two other alleles, responsible for the A2 and Ael phenotypes, may have originated by recombination, gene conversionlike events or accumulation of nucleotide substitutions. Our data indicate that different alleles could cause the same ABO variant phenotypes, and that these alleles do not necessarily belong to a single evolutionary lineage.

scholarly journals Molecular genetic analysis of a hybrid gene encoding Sta glycophorin of the human erythrocyte membrane

Blood ◽  
1989 ◽  
Vol 74 (2) ◽  
pp. 836-843
Author(s):  
CH Huang ◽  
ML Guizzo ◽  
M Kikuchi ◽  
OO Blumenfeld
Keyword(s):  
Blood Group ◽  
Erythrocyte Membrane ◽  
Molecular Genetic ◽  
Dna Typing ◽  
Molecular Genetic Analysis ◽  
Blood Group System ◽  
Sequence Specificity ◽  
Antigen Specificity ◽  
Hybrid Gene ◽  
Junction Site

Sta is an antigen of the human MNSs blood group system carried by a variant glycophorin residing in the erythrocyte membrane. We examined the structure, organization, and inheritance of Sta gene identified in genomic DNA from an Oriental family. Southern blotting detected a useful genetic marker tightly linked to the Sta gene. Differential hybridization and secondary restriction analyses showed that Sta gene is a fusion hybrid of delta and alpha glycophorin genes. Genomic mapping by extensive use of synthetic oligonucleotides, with overlapping sequence specificity, allowed us to define the delta-alpha junction site and disclose the organization of the variant gene. The junction point of Sta hybrid gene is encompassed by an unexpressed exonlike sequence of the delta gene at the 5′ site, and an expressed sequence of the alpha gene spanning codons 59 through 71, at the 3′ site. Dosage quantification demonstrated the occurrence of Sta gene as a single copy in the genome. Blood group inheritance, evaluated by DNA typing, established the tight linkage of Sta to the alpha M and delta S genes. The data support a single unequal crossing-over event between misaligned delta and alpha genes on the homologous chromosomes as the mechanism for the origin of Sta gene. The Sta gene is similar in overall structure to another delta-alpha hybrid gene, Dantu, but differs from it in junction structure, copy number, gene linkage, and antigen specificity.

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