EXPRESSION OF MARKERS OF APOPTOSIS AND AUTOPHAGIA IN TRANSPLANTED SARCOMA-45 IN RATS IN CASE OF ADMINISTRATION OF FLAVONOID-CONTAINING EXTRACT OF GRATIOLA OFFICINALIS L.

e23517 Background: The relevance of studying multiple primary malignant tumors (MPMTs) is determined by poor understanding of their pathogenesis. Our purpose was to create an experimental model of synchronous MPMTs with the stimulation of malignant growth of tumors with different histostructure. Methods: The study included 20 male BALB/c Nude mice. The main group included mice with simultaneous subcutaneous inoculation of tumors: murine B16/F10 melanoma (0.5 ml of suspension diluted 1:20 in saline solution) below the left scapula and rat sarcoma 45 (0.5 million tumor cells in 0.5 ml of saline solution) below the right scapula. Control groups included males with melanoma or sarcoma inoculated at the same dosage and volume as in the main group. Results: In the model of synchronous MPMTs, tumors appeared faster than in controls: melanoma–by 3 times, sarcoma–by 2 times; their volume was larger: melanoma–by 2.2 times, sarcoma–by 3.2 times. Melanoma metastasized, in addition to typical sites (the lungs, spleen, liver), into sarcoma 45 to the side adjacent to the chest, under the tumor node. The survival of mice with MPMTs was lower. The morphological structure of melanoma metastasis into sarcoma 45 was represented by large lamellar-rounded epithelium-like cells of melanosarcoma type with transparent cytoplasm and nuclei with a high frequency of pathological mitosis figures. Bundles of elongated spindle-shaped melanocytes with processes of the cytoplasm were determined in some melanoma areas, as well as alveolar and concentric structures. Conclusions: Synchronous subcutaneous inoculation of murine B16/F10 melanoma and rat sarcoma 45 to male BALB/c Nude mice increases their malignant potential due to an exchange of “structural information"; as a result, sarcoma acquires impulses of proliferative activity of melanoma, which, in turn, adapts and “mimics” a tumor into which it is going to metastasize.

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Action of sarcolysin and asaline on incorporation of thymidine-H3 into DNA of sarcoma 45 and tissues of tumor-bearing rats

Bulletin of Experimental Biology and Medicine ◽

10.1007/bf00797148 ◽

1976 ◽

Vol 81 (5) ◽

pp. 735-738

Author(s):

V. A. Struchkov ◽

V. V. Bergol'ts

Keyword(s):

Tumor Bearing ◽

Sarcoma 45

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Levels of the IGF system components in malignant lesions of the lungs with preventive effect of 1,3-diethylbenzimidazolium triiodide in the experiment.

Journal of Clinical Oncology ◽

10.1200/jco.2021.39.15_suppl.e15095 ◽

2021 ◽

Vol 39 (15_suppl) ◽

pp. e15095-e15095

Author(s):

Lidia K. Trepitaki ◽

Irina V. Kaplieva ◽

Elena M. Frantsiyants ◽

Viktoriya L. Volkova ◽

Dmitry A. Kharagezov ◽

...

Keyword(s):

Growth Factors ◽

Antitumor Effect ◽

Process Development ◽

Control Group ◽

Preventive Effect ◽

Igf System ◽

System Components ◽

Malignant Lesions ◽

Sarcoma 45 ◽

Insulin Like Growth Factors

e15095 Background: The system of insulin-like growth factors (IGF) is involved in carcinogenesis, since it promotes proliferation and survival of tumor cells. The purpose of the study was an analysis of the dynamics of the IGF system components in the lungs of rats with antitumor effect of 1,3-diethylbenzimidazolium triiodide. Methods: The main group included male (n=27) and female (n=27) white outbred rats with sarcoma 45 inoculated into the subclavian vein (2×106 cells in 0.5 mL saline) but not developed in the lungs due to administration of 1,3-diethylbenzimidazolium triiodide (intragastrically, 0.4 mg/kg once a day according to the regimen: administration for 5 days with a 2-day interval). Control group included males (n=14) and females (n=14) with sarcoma 45 growing in the lungs without treatment. Intact groups included 5 males and 5 females. After 4, 5 and 8 weeks of the experiment animals were decapitated, and levels of IGFI, IGFII, IGFBP1, IGFBP2 and IGFBP3 were measured by ELISA in 10% lung homogenates (CUSABIO BIOTECH Co., Ltd., China). Results: The sarcoma development in the lung was accompanied by the IGFI increase by 2.4-3.0 times in males and by 4.3 times in females, and the opposite IGFII dynamics: an increase in males (by 4.6 times) and decrease in females (by 4.3 times), together with the IGFBP decline. 1,3-diethylbenzimidazolium triiodide upregulated IGFI levels in the lungs of all rats on average by 1.3 times (p<0.05) and normalized IGFII in males, while increasing it in females by 1.6 times (p<0.05), together with higher (compared to controls) IGFBP levels. Conclusions: Preventive antitumor effect of 1.3-diethylbenzimidazolium triiodide is based on the stabilization of the IGF system grossly altered during the malignant process development in the lung.

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Combined action of SHF hyperthermia and x rays on growth of sarcoma 45

Bulletin of Experimental Biology and Medicine ◽

10.1007/bf00836378 ◽

1981 ◽

Vol 91 (4) ◽

pp. 511-513

Author(s):

S. E. Ul'yanenko ◽

B. P. Surinov ◽

I. A. Rudakov

Keyword(s):

X Rays ◽

Combined Action ◽

Sarcoma 45

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Study of Antioxidant and Membrane Resistant Peculiarities of a New Cyan Containing Lactone in Membranes of Hepatocytes with Sarcoma-45

Journal of Enzymes ◽

10.14302/issn.2690-4829.jen-19-3127 ◽

2020 ◽

Vol 1 (2) ◽

pp. 31-33

Author(s):

G.M. Galoyan ◽

P.A. Ghazaryan

Keyword(s):

Phosphatidic Acid ◽

Adenosine Triphosphatase ◽

Pla2 Activity ◽

Membrane Phospholipids ◽

Experimental Animals ◽

Atpase System ◽

Microsomal Membranes ◽

Unsaturated Lactones ◽

Sarcoma 45 ◽

Qualitative Changes

The antioxidant and membrane resistant peculiarities of a new derivative (2-cyan-3,4,4-trymethil-2-buten-4-olyd - CTBO) of cyan containing unsaturated lactones have been studied in membranes of hepatocytes with Sarcoma-45 1. The results of our previous research 1, 2, 3 showed significant changes of phospholipid (PL) exchange in hepatocytes of microsomal membranes at experimental animals vaccinated with Sarcoma-45 tumor strain. It is manifested in significant changes of quantitative and qualitative contents of membrane phospholipids separate fractions, increase of cytotoxic lysophospholipids (LPCs), phosphatidylinositol (PI) and phosphatidic acid (PA) levels, significant decrease of phosphatitylcholines (PC) and sphingomyeline (SP) contents, statistically significant changes of PL/PL ratio, peroxidation ratio intensity, dramatic increase of phospholipase A2 (PLA2)activity, quantitative and qualitative changes of adenyl nucleotides, as well as disorders of adenosine triphosphatase (ATPase) system activity 3, 4, 5, 6, 7.

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Effect of flavonoid-containing extracts on the growth of transplanted sarcoma 45, peripheral blood and bone marrow condition after oral and intramuscular administration in rats

Russian Open Medical Journal ◽

10.15275/rusomj.2017.0304 ◽

2017 ◽

Vol 6 (3) ◽

pp. e0304 ◽

Cited By ~ 6

Author(s):

Nikita Navolokin ◽

Dmitry Mudrak ◽

Alla Bucharskaya ◽

Olga Matveeva ◽

Sergey Tychina ◽

...

Keyword(s):

Bone Marrow ◽

Peripheral Blood ◽

Intramuscular Administration ◽

Sarcoma 45

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Levels of growth factors in the lungs affected by cancer with preventive effect of 1,3-diethylbenzimidazolium triiodide in the experiment

Research n Practical Medicine Journal ◽

10.17709/2409-2231-2020-7-4-3 ◽

2020 ◽

Vol 7 (4) ◽

pp. 27-35

Author(s):

E. M. Frantsiyants ◽

I. V. Kaplieva ◽

L. K. Trepitaki

Keyword(s):

Growth Factors ◽

Subclavian Vein ◽

Antitumor Effect ◽

Control Group ◽

Intragastric Administration ◽

Vegf Receptors ◽

Malignant Cells ◽

Preventive Effect ◽

Males And Females ◽

Sarcoma 45

Purpose of the study. Analyzing the dynamics of VEGF-А, TGF-β and their receptors in the lung tissues in rats with antitumor effect of 1,3-diethylbenzimidazolium triiodide (Stellanin).Material and methods. The study included white outbred rats weighing 180–220 g. The main group included males (n=27) and females (n=27) with sarcoma 45 (s45) inoculated into the subclavian vein but not developed in the lungs (2×106 cells in 0.5 ml of saline) due to the subsequent intragastric administration of Stellanin (0.4 mg/kg once a day) according to an intermittent scheme: administration for 5 days and a break for 2 days. The control group included males (n=14) and females (n=14) without treatment with growing s45 in the lungs. Intact groups included 5 males and 5 females. After 4, 5 and 8 weeks of the experiment animals were decapitated, and levels of VEGF-A, sVEGF-R1, sVEGF-R2, TGF-β and sTGFβR2 were measured in 10% lung homogenates by ELISA (CUSABIO BIOTECH Co., Ltd., China).Results. Lung tissues of intact females showed 1.4 times (p<0.05) lower VEGF-А and 3.3 times higher sVEGF-R1, compared to males. The development of tumors in all control rats was accompanied by the VEGF-А increase (by 1.6–3.0 times) and the TGF-β reduction (by 3 times). The dynamics of both VEGF receptors differed in males and females. The levels of sVEGF-R1 in males increased by 1.5 times (p<0.05), while in females it decreased by 1.8 times (p<0.05), and as a result, the levels became similar in all animals. The levels of sVEGF-R2 in males decreased by 2 times, and in females it increased by 1.4 times (p<0.05), so the sVEGF-R2 content in females became 2.4 times higher than in males. In two-thirds of rats, Stellanin prevented s45 development in the lungs due to inhibition of VEGF-A growth by more than 2.0 times and an increase in concentrations of sVEGF-R1 by 10.0 times and TGF-β by 6.0 times, together with normalization of sVEGF-R2 and sTGFβR2.Conclusions. Stellanin prevents the development of malignant process in the lungs by inhibiting neoangiogenesis (deficiency of VEGF-A and excess of sVEGF-R1) and suppressing the proliferation of malignant cells (TGF-β growth).

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