Reference intervals for mean platelet volume and immature platelet fraction determined on a sysmex XE5000 hematology analyzer

Context.—Reticulated platelets are platelets recently released from the bone marrow, and they can serve as a noninvasive indicator of recent megakaryopoietic activity. Widespread clinical use has been hampered by laborious methods and lack of standardization. Recently, a fully automated method was released on the Abbott CELL-DYN Sapphire hematology analyzer. Objective.—To establish reference ranges for reticulated platelets. Secondary aims were to investigate associations between reticulated platelets and other platelet parameters like mean platelet volume, plateletcrit, and platelet distribution width. Design.—Reticulated platelets and other platelet parameters were measured in an unselected cohort of 8089 subjects visiting a primary health care laboratory. The reticulated platelet data were analyzed using the Bhattacharya technique. In addition, a nonparametric method was used in selected subjects with normal platelet counts for providing reference ranges. Results.—Reticulated platelets ranged from 0.4% to 6.0% or from 1 to 18 × 103/μL. Reticulated platelets increased significantly with the subjects' age. Statistically, males had slightly higher values than females, but the differences were negligible. Reticulated platelets were positively correlated with platelet count and negatively with mean platelet volume. Conclusions.—Reference ranges have been established for reticulated platelets as measured on the CELL-DYN Sapphire hematology analyzer. There were no relevant differences between the sexes, but there was a clear effect of age. An individual's reticulated platelets are associated with the platelet count as well as mean platelet volume.

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Studies on Reference Intervals for Platelet Counts in Three Cities in China and one in Japan

Thrombosis and Haemostasis ◽

10.1055/s-0037-1613162 ◽

2002 ◽

Vol 88 (07) ◽

pp. 111-114 ◽

Cited By ~ 3

Author(s):

Changgeng Ruan ◽

Yun Wu ◽

Tokuhiro Okada ◽

Shigemi Motoi ◽

Tamiaki Kondo ◽

...

Keyword(s):

Platelet Count ◽

Mean Platelet Volume ◽

Healthy Subjects ◽

Hematological Parameters ◽

Reference Interval ◽

Reference Intervals ◽

Sichuan Province ◽

Jiangsu Province ◽

Platelet Volume ◽

Platelet Counts

SummaryHematological parameters including platelet counts, etc. were determined in 1,140 healthy subjects living in four cities: Suzhou (Jiangsu Province), Chengdu (Sichuan Province) and Harbin (Heilongjang Province) in China, and Kobe in Japan. Then, the reference intervals for platelet counts were calculated and compared. The reference interval for platelet count of subjects aged between 18 and 60 years was 60-259 × 109/L in Suzhou and 52–202 × 109/L in Chengdu, and subjects with platelet counts of 100 × 109/L or less accounted for about 30% of the subjects examined in these cities. The reference intervals in Harbin and Kobe were within the range of 150–350 × 109/L, and no subject having a platelets count of 100 × 109/L or less was detected. Mean platelet volume (MPV) determined concurrently was negatively correlated with platelet count, and the reference intervals for MPV in Chengdu and Suzhou were higher than those in Harbin and Kobe.

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False-positive flag of WBC and change of mean platelet volume (MPV) caused by K3-EDTA on the DxH 900 hematology analyzer

Scandinavian Journal of Clinical and Laboratory Investigation ◽

10.1080/00365513.2020.1824298 ◽

2020 ◽

Vol 80 (8) ◽

pp. 644-648

Author(s):

Sholhui Park ◽

Jungwon Huh ◽

Tae-Dong Jeong

Keyword(s):

False Positive ◽

Mean Platelet Volume ◽

Platelet Volume ◽

Hematology Analyzer

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Evaluation of Total Error and Validation of Reference Value on Hematology Analyzer

Jurnal Medikes (Media Informasi Kesehatan) ◽

10.36743/medikes.v8i1.270 ◽

2021 ◽

Vol 8 (1) ◽

pp. 37-46

Author(s):

Zulfikar Husni Faruq

Keyword(s):

Mean Platelet Volume ◽

Total Error ◽

Reference Value ◽

Small Sample ◽

Platelet Volume ◽

Hematology Analyzer

Pemeriksaan darah lengkap merupakan pemeriksaan screening atau merupakan uji saring sebagai pintu awal diagnosis sehingga dibutuhkan peralatan yang baik dan berkulitas. Penelitian ini bertujuan untuk Mengevaluasi Total Error dan Nilai Rujukan Hematology Analyzer. Pengukuran Total Eror (TE) dilakukan dengan menggunakan bahan kontrol komersial yang sudah diketahui konsentrasinya dan dilakukan pengambilan data sebanyak 20 data dengan menggunakan metode day to day kemudian dihitung menggunakan rumus Total Error (TE). Evaluasi nilai rujukan dilakukan menggunakan metode validasi nilai rujukan dengan sampel kecil (small sample) menggunakan sampel darah K2EDTA sebanyak 22 sampel laki laki dewasa dan 22 Wanita Dewasa sehat dan tidak dalam keadaan hamil. Dari hasil penelitian ini diketahui bahwa nilai Total Error (TE) memiliki nilai terbesar adalah pada parameter trombosit dan Mean Platelet Volume (MPV). Untuk hasil evaluasi nilai rujukan pada alat untuk laki laki dewasa yang belum disetujui adalah pada parameter WBC, HCT, MCV, RDW, RDW-SD dan MPV. Sedangkan parameter yang belum disetujui untuk nilai rujukan wanita dewasa adalah MCH, RDW-SD dan MPV.

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Clinical utility of mean platelet volume and immature platelet fraction in acute coronary syndrome

Biomedical Journal ◽

10.1016/j.bj.2018.12.005 ◽

2019 ◽

Vol 42 (2) ◽

pp. 107-115 ◽

Cited By ~ 5

Author(s):

Hsien-Li Huang ◽

Chih-Hung Chen ◽

Chia-Te Kung ◽

Yi-Chen Li ◽

Pei-Hsun Sung ◽

...

Keyword(s):

Acute Coronary Syndrome ◽

Mean Platelet Volume ◽

Clinical Utility ◽

Platelet Volume ◽

Coronary Syndrome ◽

Immature Platelet Fraction

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Reference interval for immature platelet fraction on Sysmex XN hematology analyzer: a comparison study with Sysmex XE-2100

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2014-0839 ◽

2015 ◽

Vol 53 (7) ◽

Cited By ~ 13

Author(s):

Young Jin Ko ◽

Mina Hur ◽

Hanah Kim ◽

Sang Gyeu Choi ◽

Hee-Won Moon ◽

...

Keyword(s):

Large Scale ◽

Reference Interval ◽

Reference Intervals ◽

Modular System ◽

Future Research ◽

Healthy Individuals ◽

Platelet Counts ◽

Immature Platelet Fraction ◽

Hematology Analyzer ◽

Non Parametric

AbstractRecently introduced hematology analyzer, the Sysmex XN modular system (Sysmex, Kobe, Japan), has newly adopted a florescent channel to detect platelets and immature platelet fraction (IPF). This study aimed to establish new reference intervals for %-IPF and absolute number of IPF (A-IPF) on Sysmex XN. Platelet counts, %-IPF, and A-IPF were also compared between Sysmex XN and XE-2100 systems (Sysmex).Except outliers, blood samples from 2104 healthy individuals and 140 umbilical cord blood were analyzed using both Sysmex XN and XE-2100. The results of two systems were compared using Bland-Altman plot. The reference intervals for %-IPF and A-IPF were defined using non-parametric percentile methods according to the Clinical and Laboratory Standard Institute guideline (C28-A3).The platelet counts, %-IPF, and A-IPF showed non-parametric distributions. The mean difference between Sysmex XN and XE-2100 in healthy individuals revealed a positive bias in platelets (+8.0×10This large-scale study demonstrates a clear difference of platelet counts and IPF between Sysmex XN and XE-2100. The new reference intervals for IPF on Sysmex XN would provide fundamental data for clinical practice and future research.

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CORRELATIONS BETWEEN MEAN PLATELET VOLUME AND IMMATURE PLATELET FRACTION TO HEMOGLOBIN A1C IN PATIENTS WITH TYPE 2 DIABETES MELLITUS

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY ◽

10.24293/ijcpml.v24i1.1148 ◽

2018 ◽

Vol 24 (1) ◽

pp. 6

Author(s):

Dian W Astuti ◽

Sony Wibisono ◽

Arifoel Hajat ◽

Sidarti Soehita

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Mean Platelet Volume ◽

Hemoglobin A1c ◽

Platelet Volume ◽

Immature Platelet Fraction ◽

Diabetes Melitus

Pasien diabetes melitus tipe 2 berkebahayaan mengalami komplikasi makro dan mikrovaskuler, yang dipengaruhi oleh kendaliglikemik. Reaktivitas trombosit berperan pada timbulnya komplikasi ini, terutama komplikasi kardiovaskuler. Tujuan penelitian iniadalah membandingkan MPV dan IPF di kendali glikemik baik dan buruk dan menentukan adanya kenasaban MPV dan IPF terhadapHbA1c. Penelitian bersifat analitik observasional dengan rancang bangun potong lintang. Sampel darah EDTA dari 43 orang pasienDM tipe 2, dikumpulkan selama Januari-Februari 2016. HbA1c diperiksa dengan Dimension RxL, sedangkan MPV dan IPF diperiksadengan Sysmex XN-1000. Rerata nilai MPV 10,36±0,84 fL, rerata nilai IPF 4,22±2,29%. Uji perbedaan nilai MPV menurut kendaliglikemik didapatkan p=0,494, uji perbedaan IPF didapatkan p=0,462. Uji kenasaban Pearson antara IPF dan MPV didapatkanr=0,877 (p<0,0001), MPV dan HbA1c didapatkan r=0,018 (p=0,907), IPF dan HbA1c didapatkan r=0,128 (p=0,414). Penelitian inimenunjukkan rerata MPV berada dalam rentang normal, sedangkan rerata IPF meningkat, namun tak terdapat perbedaan bermaknanilai MPV dan IPF di kendali glikemik baik dan buruk. MPV dan IPF pada penelitian ini tak bernasab dengan HbA1c.

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