Clinical Use of Immunohistopathologic Methods for the Diagnosis of Cytomegalovirus Hepatitis in Human Liver Allograft Biopsy Specimens

1994 ◽  
Vol 29 (6) ◽  
pp. 553-560 ◽  
Author(s):  
L. M. Barkholt ◽  
A. Ehrnst ◽  
B. Veress
Keyword(s):  
Human Liver ◽  
Clinical Use ◽  
Liver Allograft ◽  
Biopsy Specimens ◽  
Allograft Biopsy ◽  
Cytomegalovirus Hepatitis

scholarly journals Glomerular Neovascularization in Nondiabetic Renal Allograft Is Associated with Calcineurin Inhibitor Toxicity

Nephron ◽  
2020 ◽  
pp. 1-6
Author(s):  
Anri Sawada ◽  
Masayoshi Okumi ◽  
Shigeru Horita ◽  
Kohei Unagami ◽  
Sekiko Taneda ◽  
...  
Keyword(s):  
Diabetic Kidney Disease ◽  
Renal Allograft ◽  
Calcineurin Inhibitor ◽  
Clinical Information ◽  
Renal Allografts ◽  
Biopsy Specimens ◽  
Allograft Biopsy ◽  
Time Periods ◽  
Medical University ◽  
Trough Levels

<b><i>Introduction:</i></b> Extra efferent arterioles, also known as polar vasculosis (PV), are often observed in the glomerular vascular pole and are associated with glomerular hypertrophy, indicating early recurrent diabetic kidney disease (DKD) in renal allografts. However, its significance in patients without diabetes remains uncertain. <b><i>Methods:</i></b> A total of 9,004 renal allograft biopsy specimens obtained between January 2007 and December 2017 at Tokyo Women’s Medical University were retrospectively analyzed to examine the clinical and pathological significance of PV in renal allografts. PV was identified in 186 biopsy specimens obtained from 165 patients. The PV group comprised 46 patients; 35 patients without DKD and 11 patients with DKD as the initial cause of ESRD, whose clinical information was available and treated with the calcineurin inhibitor (CNI) tacrolimus. The non-PV group comprising patients with renal allografts matched for age and postoperative day included 93 patients without DKD and 16 patients with DKD as the initial cause of ESRD. <b><i>Results:</i></b> In patients with nondiabetic renal allografts, systolic blood pressure was significantly higher in the PV group than in the non-PV group. The trough tacrolimus levels during the overall study period and at 2 weeks, 1 month, and 2 years after transplantation were significantly higher in the PV group compared with the non-PV group. Glomerulomegaly was significantly more common. Moreover, ah and aah scores in Banff score were significantly higher in the PV group than in the non-PV group. In those with diabetic renal allografts, although the clinical parameters and tacrolimus trough levels in all time periods were not significantly different between the PV and non-PV groups, the ah score was significantly higher in the PV group. <b><i>Conclusion:</i></b> PV was associated with CNI toxicity in nondiabetic but not in diabetic renal allografts. The pathogenesis of PV in renal allografts is considered to be multifactorial.

scholarly journals Analytical subcellular fractionation of needle-biopsy specimens from human liver

1978 ◽  
Vol 174 (2) ◽  
pp. 435-446 ◽  
Author(s):  
T J Peters ◽  
C A Seymour
Keyword(s):  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Density Gradient ◽  
Needle Biopsy ◽  
Human Liver ◽  
Resolving Power ◽  
Equilibrium Density ◽  
Marker Enzyme ◽  
Acid Hydrolases ◽  
Biopsy Specimens

1. Fragments (2-20 mg wet wt.) of closed needle-biopsy specimens from human liver were disrupted in iso-osmotic sucrose and subjected to low-speed centrifugation. The supernatant was layered on a linear sucrose-density gradient in the Beaufay small-volume automatic zonal rotor. The following organelles, with equilibrium densities (g/ml) and principal marker enzyme shown in parentheses, were resolved: plasma membrane (1.12-1.14; 5′-nucleotidase); lysosomes (1.15-1.20; N-acetyl-beta-glucosaminidase); mitochondria (1.20; malate dehydrogenase); endoplasmic reticulum (1.17-1.21; neutral alpha-glucosidase); peroxisomes (1.22-1.24; catalase). 2. The distribution of particulate alkaline phosphatase and, to a lesser degree, leucine 2-naphthylamidase followed that of 5′-nucleotidase. gamma-Glutamyltransferase was associated with membranes of significantly higher equilibrium density than was 5′-nucleotidase. 3. The distribution of 12 acid hydrolases was determined in the density-gradient fractions. beta-Glucosidase had a predominantly cytosolic localization, but the other enzymes showed a broad distribution of activity throughout the gradient. Evidence was presented for two populations of lysosomes with equilibrium densities of 1.15 and 1.20 g/ml, but containing differing amounts of each enzyme. Further evidence of lysosomal heterogeneity was demonstrated by studying the distribution of isoenzymes of hexosaminidase and of acid phosphatase. 4. The resolving power of the centrifugation procedure can be further enhanced with membrane perturbants. Digitonin (0.12 mM) selectively disrupted lysosomes, markedly increased the equilibrium density of plasma-membrane components and lowered the density of the endoplasmic reticulum, but did not affect the mitochondria or peroxisomes. Pyrophosphate (15 mM) selectively lowered the equilibrium density of the endoplasmic reticulum.

MORPHOMETRIC INFLAMMATORY CELL ANALYSIS OF HUMAN LIVER ALLOGRAFT BIOPSIES

1991 ◽  
Vol 51 (4) ◽  
pp. 873-876 ◽  
Author(s):  
PRESTON F. FOSTER ◽  
HOWARD N. SANKARY ◽  
JAMES W. WILLIAMS ◽  
ACHYUT BHATTACHARYYA ◽  
JAMES COLEMAN ◽  
...  
Keyword(s):  
Human Liver ◽  
Inflammatory Cell ◽  
Cell Analysis ◽  
Liver Allograft

scholarly journals Simultaneous staining of cytoplasmic iron and collagen matrix in human liver biopsy specimens

10.4081/1658 ◽  
2009 ◽  
Vol 46 (1) ◽  
pp. 101 ◽  
Author(s):  
F Grizzi ◽  
G Ceva-Grimaldi ◽  
B Franceschini ◽  
M Roncalli ◽  
M Chiriva-Internati ◽  
...  
Keyword(s):  
Liver Biopsy ◽  
Human Liver ◽  
Collagen Matrix ◽  
Biopsy Specimens

Determination of bromsulphthalein-glutathione conjugating enzyme activity in needle biopsy specimens of human liver

1973 ◽  
Vol 47 (2) ◽  
pp. 133-137 ◽  
Author(s):  
D.V. Datta ◽  
Sarban Singh ◽  
A.K.S. Samanta ◽  
S. Saha ◽  
M. Mukherjee ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Needle Biopsy ◽  
Human Liver ◽  
Biopsy Specimens ◽  
Conjugating Enzyme
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