CA125 levels in matched samples of amniotic fluid, extra-embryonic coelomic fluid and maternal serum in the first trimester of pregnancy

1992 ◽  
Vol 12 (3) ◽  
pp. 156-158
Author(s):  
J. Campbell ◽  
Muriel Kitau ◽  
P. Cass ◽  
N. Wathen ◽  
T. Chard
Keyword(s):  
Amniotic Fluid ◽  
First Trimester ◽  
Maternal Serum ◽  
Coelomic Fluid ◽  
First Trimester Of Pregnancy ◽  
Matched Samples

scholarly journals Concentrations of vitamins A and E in amniotic fluid, extraembryonic coelomic fluid, and maternal serum in the first trimester of pregnancy.

1994 ◽  
Vol 71 (1) ◽  
pp. F49-F50 ◽  
Author(s):  
J Campbell ◽  
N C Wathen ◽  
I Merryweather ◽  
R Abbott ◽  
D Muller ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
High Performance ◽  
Transvaginal Ultrasound ◽  
First Trimester ◽  
Normal Pregnancy ◽  
Maternal Serum ◽  
Coelomic Fluid ◽  
Cholesterol Ratio ◽  
Paired Samples ◽  
First Trimester Of Pregnancy

Paired samples of amniotic fluid and coelomic fluid were obtained by transvaginal ultrasound guided amniocentesis from 15 women with an ultrasonographically normal pregnancy between 8 and 12 weeks' gestation. Vitamins A and E were measured in the two pregnancy fluids and in maternal serum by high performance liquid chromatography with detection by ultraviolet absorption and fluorimetry respectively. Concentrations of vitamins A and E were higher in maternal serum than in coelomic fluid and were generally undetectable in amniotic fluid. All differences in concentration were significant. The vitamin E/cholesterol ratio was similar in maternal serum and coelomic fluid. No correlation was shown between the vitamin concentrations in the three fluids. These findings suggest that the coelomic cavity plays a part in the maternofetal exchange of these vitamins.

Human chorionic gonadotrophin and subunit composition of maternal serum and coelomic and amniotic fluids in the first trimester of pregnancy

1992 ◽  
Vol 135 (3) ◽  
pp. 563-569 ◽  
Author(s):  
R. K. Iles ◽  
N. C. Wathen ◽  
D. J. Campbell ◽  
T. Chard
Keyword(s):  
First Trimester ◽  
Maternal Serum ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Subunit Composition ◽  
Major Constituent ◽  
Coelomic Fluid ◽  
Α Subunit ◽  
First Trimester Of Pregnancy ◽  
Β Hcg

ABSTRACT Sixteen matched samples of first trimester amniotic fluid (AF), extraembryonic coelomic fluid (EECF) and maternal serum (MS) were assayed for intact human chorionic gonadotrophin (hCG) and free subunits. Total β-hCG (free β-subunit and intact hCG) levels in the EECF (median 410 kIU/l) were 61 times greater than levels in AF (median 6·73 kIU/l) and 2·8 times greater than in MS (median 141·5 kIU/l). Levels of intact hCG in the EECF (median 245 kIU/l) were 142 times greater than in AF (median 1·73 kIU/l) and 1·6 times greater than in MS (median 157 kIU/l). Free α-subunit levels in EECF (median 17·3 mg/l) were 66 times greater than in AF (median 0·262 mg/l) and 12 times greater than in MS (median 1·3 mg/l). Virtually all of the total β-hCG immunoreactivity in MS can be attributed to intact hCG, but only 60% of total β-hCG in the EECF and 20% of that in AF can be accounted for by the intact hormone. In both EECF and AF the free α-subunit was a major constituent; on a molar basis the ratio of free α:free β:intact hCG was 1:1·2:0·3 in AF, 1:0·6:0·5 in EECF and 1:0:5 in MS. Chromatography of MS, EECF and AF on Sephadex G-100 confirmed the hCG and subunit composition of the fluids. On the basis of these findings it seems likely that previous studies showing very high levels of hCG in AF during the first trimester may have incorrectly sampled the EECF. In reality, the levels of total hCG (and free subunits) are low in the AF, and only 20% is intact hCG. In both AF and EECF the free subunits may have been derived by dissociation of intact hormone, or possibly by independent synthesis. These and other findings suggest that either the amnion acts as a barrier to the transfer of proteins or that there may be dynamic removal from this compartment. By contrast, the EECF might act as a relatively stable reservoir for these proteins. Journal of Endocrinology (1992) 135, 563–569

Levels of insulin-like growth factor-binding protein-2 and insulin-like growth factor-II in maternal serum, amniotic fluid and extraembryonic coelomic fluid at 9–20 weeks of pregnancy

1994 ◽  
Vol 142 (2) ◽  
pp. 379-383 ◽  
Author(s):  
T Chard ◽  
W F Blum ◽  
J Brunjes ◽  
D J Campbell ◽  
N C Wathen
Keyword(s):  
Growth Factor ◽  
Amniotic Fluid ◽  
Binding Protein ◽  
First Trimester ◽  
Maternal Serum ◽  
Coelomic Fluid ◽  
Insulin Like Growth Factor ◽  
Whitney Test ◽  
Mann Whitney Test ◽  
Factor Ii

Abstract Insulin-like growth factor-II (IGF-II) and IGF-binding protein-2 (IGFBP-2) were measured in amniotic fluid, extraembryonic fluid and maternal serum from 20 women with apparently normal first trimester pregnancies prior to termination. A further 111 specimens of amniotic fluid were collected from women at 10–20 weeks of pregnancy. Levels of IGFBP-2 were similar in coelomic fluid and maternal serum. Levels in amniotic fluid were lower than those in serum and coelomic fluid (Mann–Whitney test; P=0·0002 and P<0·0001 respectively). The levels of IGF-II were much higher in maternal serum than in coelomic fluid, and higher in the latter than in amniotic fluid (Mann–Whitney test; P<0·0001 for both situations). The levels of IGFBP-2 were relatively low at 10–11 weeks (medians 19·8 and 61·1 μg/l) but thereafter increased to 20 weeks (median 1400 μg/l). The levels of IGF-II showed a similar pattern. The findings suggest that the role of IGF-II and IGFBP-2 in the regulation of growth or differentiation of the fetus or of its surrounding membranes may change with advancing pregnancy. Journal of Endocrinology (1994) 142, 379–383

scholarly journals False Positive Values of Biomarkers of Prenatal Screening on Chromosomopathy as Indicators of a Risky Pregnancy

2011 ◽  
Vol 30 (2) ◽  
pp. 126-130 ◽  
Author(s):  
Jasmina Durković ◽  
Luka Anđelić ◽  
Bojana Mandić ◽  
Denis Lazar
Keyword(s):  
Genetic Screening ◽  
False Positive ◽  
Prenatal Screening ◽  
Protein A ◽  
First Trimester ◽  
Maternal Serum ◽  
Fetal Hypoxia ◽  
Beta Hcg ◽  
First Trimester Of Pregnancy ◽  
Fetal Karyotype

False Positive Values of Biomarkers of Prenatal Screening on Chromosomopathy as Indicators of a Risky PregnancyGenetic screening on chromosomopathy has been performed on 2000 pregnant women in their first trimester of pregnancy by determining Pregnancy associated plasma protein-A and free-beta HCG biomarkers in maternal serum. After obtaining a normal fetal karyotype, the pathological values of the biomarkers have been correlated with other pregnancy disorders, and the possible causes of the positive genetic screening have been tested. 340 false positive biomarkers (17%) have been detected. The increased free-beta HCG (48.24%) had a significant influence. A significant correlation (p > 0.01) between the increased free-beta HCG and bleeding during pregnancy has been established. Complications occurred in 78.52% pregnancies with pathological biomarkers, MISSed in 13.82%, miscarriages in 10.88%, induced pregnancy terminations caused by fetal anomalies in 8.82% and births with disturbed fetal vitality in 45%. The research results have shown a significant correlation (p > 0.01) between the increased value of the free-beta HCG biomarkers and fetal hypoxia. The false positive genetic screening, caused by the increased free-beta HCG, can indicate placental dysfunction and fetal vitality disruption.

scholarly journals Role of Maternal Serum Glycodelin as Predictor of Ectopic Pregnancy in First Trimester

2018 ◽  
Vol 7 (4) ◽  
pp. 467-470
Author(s):  
Wasan Wajdi Ibrahim ◽  
Afraa Mahjoob Al-Naddawi ◽  
Hayder A. Fawzi
Keyword(s):  
Ectopic Pregnancy ◽  
Predictive Value ◽  
First Trimester ◽  
Maternal Serum ◽  
Intrauterine Pregnancy ◽  
Receiver Operator Curve Analysis ◽  
Medical City ◽  
First Trimester Of Pregnancy ◽  
Control Study

Objectives: Assessment of glycodelin (GD) as a marker for unruptured ectopic pregnancy (EP) in the first trimester of pregnancy. Materials and Methods: This case-control study was conducted during June 2016 to May 2017 in the Obstetrics and Gynecological Department of Baghdad University at Baghdad teaching hospital/medical city complex. In this study, 100 pregnant women in their first trimester of pregnancy were included after clinical and ultrasonic findings. Results: Based on the results, GD levels in EP were significantly lower than those with normal intrauterine pregnancy (1.58 ± 1.18 vs. 30.1 ± 11.9). In addition, using receiver operator curve analysis, the cut-off GD level of 9.5 and less had acceptable validity results (100% sensitivity, 100% specificity, 95% positive predictive value, 100% negative predictive value, and accuracy 100%) to predict EP. Conclusions: In general, serum GD is considered as an excellent predictor of unruptured EP.

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